Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21265 and BWI76_RS21270 are separated by 53 nucleotides BWI76_RS21270 and BWI76_RS21275 overlap by 11 nucleotides BWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotides BWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides
BWI76_RS21265: BWI76_RS21265 - phosphoribosylformylglycinamidine synthase, at 4,452,929 to 4,456,816
_RS21265
BWI76_RS21270: BWI76_RS21270 - phosphoribosylformyl-glycineamide synthetase, at 4,456,870 to 4,457,001
_RS21270
BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607
_RS21275
BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149
_RS21280
BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804
_RS21285
Position (kb)
4456
4457
4458
4459 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4455.995 kb on - strand, within BWI76_RS21265 at 4456.010 kb on + strand, within BWI76_RS21265 at 4456.176 kb on + strand, within BWI76_RS21265 at 4456.509 kb on + strand at 4456.517 kb on - strand at 4456.534 kb on - strand at 4456.609 kb on + strand at 4456.609 kb on + strand at 4456.737 kb on - strand at 4456.875 kb on - strand at 4457.009 kb on - strand at 4457.480 kb on + strand, within BWI76_RS21275 at 4457.524 kb on + strand, within BWI76_RS21275 at 4457.617 kb on - strand, within BWI76_RS21275 at 4457.684 kb on + strand, within BWI76_RS21275 at 4457.738 kb on - strand, within BWI76_RS21275 at 4458.374 kb on + strand, within BWI76_RS21275 at 4458.380 kb on + strand, within BWI76_RS21275 at 4458.382 kb on - strand, within BWI76_RS21275 at 4458.492 kb on - strand at 4458.607 kb on + strand at 4459.171 kb on + strand at 4459.398 kb on + strand, within BWI76_RS21285
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 4,455,995 - BWI76_RS21265 0.79 +0.2 4,456,010 + BWI76_RS21265 0.79 -0.0 4,456,176 + BWI76_RS21265 0.84 -0.1 4,456,509 + -0.2 4,456,517 - +1.1 4,456,534 - -0.6 4,456,609 + +0.1 4,456,609 + +0.3 4,456,737 - -0.7 4,456,875 - -0.3 4,457,009 - -2.8 4,457,480 + BWI76_RS21275 0.30 -2.0 4,457,524 + BWI76_RS21275 0.33 +0.7 4,457,617 - BWI76_RS21275 0.39 -2.4 4,457,684 + BWI76_RS21275 0.43 -0.4 4,457,738 - BWI76_RS21275 0.46 +0.6 4,458,374 + BWI76_RS21275 0.86 -0.3 4,458,380 + BWI76_RS21275 0.86 -1.2 4,458,382 - BWI76_RS21275 0.86 -0.9 4,458,492 - -0.3 4,458,607 + +1.3 4,459,171 + -0.7 4,459,398 + BWI76_RS21285 0.36 +0.7
Or see this region's nucleotide sequence