Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12545 and BWI76_RS12550 are separated by 14 nucleotides BWI76_RS12550 and BWI76_RS12555 overlap by 4 nucleotides BWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotides BWI76_RS12560 and BWI76_RS12565 overlap by 4 nucleotides
BWI76_RS12545: BWI76_RS12545 - hypothetical protein, at 2,621,007 to 2,621,966
_RS12545
BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514
_RS12550
BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035
_RS12555
BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517
_RS12560
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-1
0
1
2 at 2622.111 kb on + strand, within BWI76_RS12550 at 2622.264 kb on - strand, within BWI76_RS12550 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.806 kb on + strand, within BWI76_RS12555 at 2622.814 kb on - strand, within BWI76_RS12555 at 2622.838 kb on - strand, within BWI76_RS12555 at 2623.224 kb on + strand at 2623.615 kb on + strand at 2623.691 kb on + strand, within BWI76_RS12565 at 2623.995 kb on + strand, within BWI76_RS12565 at 2624.000 kb on + strand, within BWI76_RS12565
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 2,622,111 + BWI76_RS12550 0.24 +0.4 2,622,264 - BWI76_RS12550 0.53 +1.9 2,622,699 - BWI76_RS12555 0.36 -0.4 2,622,699 - BWI76_RS12555 0.36 -0.9 2,622,806 + BWI76_RS12555 0.56 -0.8 2,622,814 - BWI76_RS12555 0.58 +0.0 2,622,838 - BWI76_RS12555 0.62 -0.9 2,623,224 + +1.5 2,623,615 + -0.1 2,623,691 + BWI76_RS12565 0.14 +0.0 2,623,995 + BWI76_RS12565 0.39 +0.1 2,624,000 + BWI76_RS12565 0.40 -0.1
Or see this region's nucleotide sequence