Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11515 and BWI76_RS11520 are separated by 42 nucleotides BWI76_RS11520 and BWI76_RS11525 are separated by 60 nucleotides BWI76_RS11525 and BWI76_RS11530 are separated by 100 nucleotides BWI76_RS11530 and BWI76_RS11535 are separated by 324 nucleotides
BWI76_RS11515: BWI76_RS11515 - peptide-methionine (R)-S-oxide reductase, at 2,423,817 to 2,424,230
_RS11515
BWI76_RS11520: BWI76_RS11520 - hypothetical protein, at 2,424,273 to 2,424,548
_RS11520
BWI76_RS11525: BWI76_RS11525 - HNH endonuclease, at 2,424,609 to 2,424,953
_RS11525
BWI76_RS11530: BWI76_RS11530 - chitinase, at 2,425,054 to 2,426,307
_RS11530
BWI76_RS11535: BWI76_RS11535 - putative oxidoreductase, Zn-dependent and NAD(P)-binding protein, at 2,426,632 to 2,427,708
_RS11535
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-1
0
1 at 2424.443 kb on + strand, within BWI76_RS11520 at 2424.573 kb on + strand at 2424.759 kb on + strand, within BWI76_RS11525 at 2424.772 kb on - strand, within BWI76_RS11525 at 2425.060 kb on - strand at 2425.203 kb on + strand, within BWI76_RS11530 at 2425.553 kb on + strand, within BWI76_RS11530 at 2425.558 kb on + strand, within BWI76_RS11530 at 2425.566 kb on - strand, within BWI76_RS11530 at 2425.605 kb on + strand, within BWI76_RS11530 at 2425.646 kb on - strand, within BWI76_RS11530 at 2425.753 kb on + strand, within BWI76_RS11530 at 2425.924 kb on + strand, within BWI76_RS11530 at 2426.128 kb on - strand, within BWI76_RS11530 at 2426.467 kb on + strand at 2427.190 kb on - strand, within BWI76_RS11535
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 2,424,443 + BWI76_RS11520 0.62 +0.5 2,424,573 + -0.6 2,424,759 + BWI76_RS11525 0.43 +0.1 2,424,772 - BWI76_RS11525 0.47 -0.1 2,425,060 - +1.5 2,425,203 + BWI76_RS11530 0.12 -1.2 2,425,553 + BWI76_RS11530 0.40 -0.7 2,425,558 + BWI76_RS11530 0.40 -0.1 2,425,566 - BWI76_RS11530 0.41 -0.6 2,425,605 + BWI76_RS11530 0.44 +0.2 2,425,646 - BWI76_RS11530 0.47 -0.2 2,425,753 + BWI76_RS11530 0.56 -1.1 2,425,924 + BWI76_RS11530 0.69 -0.1 2,426,128 - BWI76_RS11530 0.86 -0.7 2,426,467 + +0.3 2,427,190 - BWI76_RS11535 0.52 -0.4
Or see this region's nucleotide sequence