Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10550

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10540 and BWI76_RS10545 are separated by 227 nucleotidesBWI76_RS10545 and BWI76_RS10550 overlap by 1 nucleotidesBWI76_RS10550 and BWI76_RS10555 overlap by 4 nucleotidesBWI76_RS10555 and BWI76_RS10560 are separated by 9 nucleotides BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861 _RS10540 BWI76_RS10545: BWI76_RS10545 - hypothetical protein, at 2,228,089 to 2,228,472 _RS10545 BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869 _RS10550 BWI76_RS10555: BWI76_RS10555 - hypothetical protein, at 2,229,866 to 2,230,054 _RS10555 BWI76_RS10560: BWI76_RS10560 - gamma-glutamyltransferase, at 2,230,064 to 2,231,647 _RS10560 Position (kb) 2228 2229 2230Strain fitness (log2 ratio) -1 0 1at 2227.618 kb on - strand, within BWI76_RS10540at 2227.644 kb on - strand, within BWI76_RS10540at 2227.667 kb on - strand, within BWI76_RS10540at 2227.754 kb on - strand, within BWI76_RS10540at 2227.888 kb on + strandat 2227.909 kb on - strandat 2228.048 kb on + strandat 2228.077 kb on - strandat 2228.117 kb on - strandat 2228.716 kb on - strand, within BWI76_RS10550at 2229.024 kb on + strand, within BWI76_RS10550at 2229.082 kb on + strand, within BWI76_RS10550at 2229.087 kb on + strand, within BWI76_RS10550at 2229.203 kb on + strand, within BWI76_RS10550at 2230.028 kb on - strand, within BWI76_RS10555at 2230.180 kb on + strandat 2230.188 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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2,227,618 - BWI76_RS10540 0.74 -0.5
2,227,644 - BWI76_RS10540 0.77 -0.5
2,227,667 - BWI76_RS10540 0.79 +0.3
2,227,754 - BWI76_RS10540 0.88 -0.5
2,227,888 + +0.2
2,227,909 - -0.2
2,228,048 + -0.1
2,228,077 - +0.2
2,228,117 - +0.5
2,228,716 - BWI76_RS10550 0.17 -0.3
2,229,024 + BWI76_RS10550 0.39 -1.1
2,229,082 + BWI76_RS10550 0.44 -1.6
2,229,087 + BWI76_RS10550 0.44 -0.3
2,229,203 + BWI76_RS10550 0.52 -0.3
2,230,028 - BWI76_RS10555 0.86 -1.1
2,230,180 + -0.0
2,230,188 - +0.2

Or see this region's nucleotide sequence