Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10250

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10245 and BWI76_RS10250 are separated by 68 nucleotidesBWI76_RS10250 and BWI76_RS10255 overlap by 17 nucleotidesBWI76_RS10255 and BWI76_RS10260 are separated by 10 nucleotides BWI76_RS10245: BWI76_RS10245 - aspartate aminotransferase, at 2,169,600 to 2,170,808 _RS10245 BWI76_RS10250: BWI76_RS10250 - glutamine amidotransferase, at 2,170,877 to 2,171,974 _RS10250 BWI76_RS10255: BWI76_RS10255 - dihydrodipicolinate synthase family protein, at 2,171,958 to 2,172,881 _RS10255 BWI76_RS10260: BWI76_RS10260 - creatinase, at 2,172,892 to 2,174,061 _RS10260 Position (kb) 2170 2171 2172Strain fitness (log2 ratio) -2 -1 0 1at 2170.526 kb on + strand, within BWI76_RS10245at 2170.729 kb on + strandat 2171.157 kb on - strand, within BWI76_RS10250at 2171.184 kb on - strand, within BWI76_RS10250at 2171.448 kb on + strand, within BWI76_RS10250at 2171.456 kb on - strand, within BWI76_RS10250at 2171.456 kb on - strand, within BWI76_RS10250at 2171.552 kb on + strand, within BWI76_RS10250at 2171.900 kb on - strandat 2172.023 kb on - strandat 2172.023 kb on - strandat 2172.023 kb on - strandat 2172.668 kb on - strand, within BWI76_RS10255at 2172.750 kb on + strand, within BWI76_RS10255at 2172.750 kb on + strand, within BWI76_RS10255

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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2,170,526 + BWI76_RS10245 0.77 -0.2
2,170,729 + -0.1
2,171,157 - BWI76_RS10250 0.26 -0.5
2,171,184 - BWI76_RS10250 0.28 -1.4
2,171,448 + BWI76_RS10250 0.52 -0.5
2,171,456 - BWI76_RS10250 0.53 -0.5
2,171,456 - BWI76_RS10250 0.53 -0.6
2,171,552 + BWI76_RS10250 0.61 -1.9
2,171,900 - -0.6
2,172,023 - -0.4
2,172,023 - +0.4
2,172,023 - +0.1
2,172,668 - BWI76_RS10255 0.77 +0.9
2,172,750 + BWI76_RS10255 0.86 +0.1
2,172,750 + BWI76_RS10255 0.86 -1.3

Or see this region's nucleotide sequence