Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02925 and BWI76_RS02930 are separated by 17 nucleotides BWI76_RS02930 and BWI76_RS02935 are separated by 257 nucleotides BWI76_RS02935 and BWI76_RS02940 are separated by 369 nucleotides
BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123
_RS02925
BWI76_RS02930: BWI76_RS02930 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 598,141 to 598,605
_RS02930
BWI76_RS02935: BWI76_RS02935 - anaerobic ribonucleoside triphosphate reductase, at 598,863 to 601,001
_RS02935
BWI76_RS02940: BWI76_RS02940 - RpiR family transcriptional regulator, at 601,371 to 602,114
_RS02940
Position (kb) 598
599
600
601
602 Strain fitness (log2 ratio) -1
0
1
2
3 at 597.921 kb on - strand, within BWI76_RS02925 at 598.153 kb on - strand at 598.281 kb on + strand, within BWI76_RS02930 at 598.521 kb on + strand, within BWI76_RS02930 at 598.552 kb on + strand, within BWI76_RS02930 at 598.559 kb on + strand at 598.591 kb on - strand at 598.853 kb on - strand at 598.924 kb on + strand at 599.019 kb on + strand at 599.027 kb on - strand at 599.118 kb on + strand, within BWI76_RS02935 at 599.143 kb on + strand, within BWI76_RS02935 at 599.144 kb on + strand, within BWI76_RS02935 at 599.152 kb on - strand, within BWI76_RS02935 at 599.209 kb on + strand, within BWI76_RS02935 at 599.243 kb on + strand at 599.251 kb on - strand, within BWI76_RS02935 at 599.261 kb on - strand, within BWI76_RS02935 at 599.376 kb on + strand, within BWI76_RS02935 at 599.381 kb on + strand, within BWI76_RS02935 at 599.381 kb on + strand, within BWI76_RS02935 at 599.388 kb on + strand, within BWI76_RS02935 at 599.389 kb on - strand, within BWI76_RS02935 at 599.465 kb on + strand, within BWI76_RS02935 at 599.610 kb on - strand, within BWI76_RS02935 at 599.610 kb on - strand, within BWI76_RS02935 at 599.788 kb on - strand, within BWI76_RS02935 at 599.802 kb on + strand, within BWI76_RS02935 at 599.815 kb on - strand, within BWI76_RS02935 at 599.959 kb on - strand, within BWI76_RS02935 at 600.233 kb on - strand, within BWI76_RS02935 at 600.331 kb on + strand, within BWI76_RS02935 at 600.406 kb on - strand, within BWI76_RS02935 at 600.580 kb on + strand, within BWI76_RS02935 at 600.620 kb on + strand, within BWI76_RS02935 at 600.620 kb on + strand, within BWI76_RS02935 at 600.670 kb on + strand, within BWI76_RS02935 at 600.678 kb on - strand, within BWI76_RS02935 at 600.755 kb on - strand, within BWI76_RS02935 at 600.997 kb on + strand at 600.997 kb on - strand at 601.009 kb on + strand at 601.049 kb on - strand at 601.115 kb on + strand at 601.156 kb on - strand at 601.285 kb on - strand at 601.395 kb on - strand at 601.442 kb on - strand at 601.446 kb on - strand, within BWI76_RS02940 at 601.535 kb on + strand, within BWI76_RS02940 at 601.699 kb on + strand, within BWI76_RS02940 at 601.887 kb on - strand, within BWI76_RS02940
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 597,921 - BWI76_RS02925 0.88 +0.8 598,153 - +0.6 598,281 + BWI76_RS02930 0.30 +0.2 598,521 + BWI76_RS02930 0.82 +0.2 598,552 + BWI76_RS02930 0.88 +0.8 598,559 + +0.1 598,591 - +0.6 598,853 - -0.1 598,924 + +0.9 599,019 + +0.6 599,027 - +0.8 599,118 + BWI76_RS02935 0.12 +0.2 599,143 + BWI76_RS02935 0.13 +0.7 599,144 + BWI76_RS02935 0.13 +0.7 599,152 - BWI76_RS02935 0.14 +0.3 599,209 + BWI76_RS02935 0.16 +0.5 599,243 + -0.1 599,251 - BWI76_RS02935 0.18 -0.1 599,261 - BWI76_RS02935 0.19 +0.2 599,376 + BWI76_RS02935 0.24 +0.7 599,381 + BWI76_RS02935 0.24 +0.2 599,381 + BWI76_RS02935 0.24 +0.4 599,388 + BWI76_RS02935 0.25 +0.7 599,389 - BWI76_RS02935 0.25 -0.6 599,465 + BWI76_RS02935 0.28 +0.7 599,610 - BWI76_RS02935 0.35 +0.5 599,610 - BWI76_RS02935 0.35 +0.7 599,788 - BWI76_RS02935 0.43 +0.4 599,802 + BWI76_RS02935 0.44 -0.8 599,815 - BWI76_RS02935 0.45 +1.0 599,959 - BWI76_RS02935 0.51 +0.6 600,233 - BWI76_RS02935 0.64 +0.5 600,331 + BWI76_RS02935 0.69 -0.1 600,406 - BWI76_RS02935 0.72 -0.3 600,580 + BWI76_RS02935 0.80 +1.1 600,620 + BWI76_RS02935 0.82 +0.4 600,620 + BWI76_RS02935 0.82 +2.1 600,670 + BWI76_RS02935 0.84 +1.0 600,678 - BWI76_RS02935 0.85 -1.2 600,755 - BWI76_RS02935 0.88 +0.6 600,997 + +3.5 600,997 - +0.2 601,009 + -0.2 601,049 - +0.3 601,115 + +1.7 601,156 - +0.8 601,285 - -0.8 601,395 - +0.1 601,442 - +0.3 601,446 - BWI76_RS02940 0.10 -0.4 601,535 + BWI76_RS02940 0.22 +0.8 601,699 + BWI76_RS02940 0.44 +0.8 601,887 - BWI76_RS02940 0.69 +0.2
Or see this region's nucleotide sequence