Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00275 and BWI76_RS00280 are separated by 5 nucleotides BWI76_RS00280 and BWI76_RS00285 are separated by 27 nucleotides BWI76_RS00285 and BWI76_RS00290 are separated by 101 nucleotides
BWI76_RS00275: BWI76_RS00275 - ribose ABC transporter ATP-binding protein RbsA, at 56,739 to 58,244
_RS00275
BWI76_RS00280: BWI76_RS00280 - ribose ABC transporter permease, at 58,250 to 59,215
_RS00280
BWI76_RS00285: BWI76_RS00285 - D-ribose ABC transporter substrate-binding protein, at 59,243 to 60,133
_RS00285
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 60,235 to 61,164
_RS00290
Position (kb) 59
60
61 Strain fitness (log2 ratio) -2
-1
0
1
2 at 58.395 kb on - strand, within BWI76_RS00280 at 58.395 kb on - strand, within BWI76_RS00280 at 58.442 kb on - strand at 58.490 kb on + strand, within BWI76_RS00280 at 58.779 kb on + strand, within BWI76_RS00280 at 58.819 kb on + strand, within BWI76_RS00280 at 58.829 kb on + strand, within BWI76_RS00280 at 59.158 kb on - strand at 59.173 kb on + strand at 59.215 kb on + strand at 59.231 kb on - strand at 59.272 kb on - strand at 59.417 kb on - strand, within BWI76_RS00285 at 59.538 kb on - strand, within BWI76_RS00285 at 59.546 kb on - strand, within BWI76_RS00285 at 59.758 kb on + strand, within BWI76_RS00285 at 59.758 kb on + strand, within BWI76_RS00285 at 59.775 kb on + strand, within BWI76_RS00285 at 59.830 kb on - strand, within BWI76_RS00285 at 60.108 kb on + strand at 60.116 kb on - strand at 60.261 kb on - strand at 60.289 kb on + strand at 60.371 kb on + strand, within BWI76_RS00290 at 60.462 kb on + strand, within BWI76_RS00290 at 60.500 kb on - strand, within BWI76_RS00290 at 60.505 kb on - strand, within BWI76_RS00290 at 60.519 kb on - strand, within BWI76_RS00290 at 60.597 kb on - strand at 60.599 kb on - strand, within BWI76_RS00290 at 60.635 kb on + strand, within BWI76_RS00290 at 60.656 kb on + strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.793 kb on + strand, within BWI76_RS00290 at 60.820 kb on - strand, within BWI76_RS00290 at 60.821 kb on - strand, within BWI76_RS00290 at 60.865 kb on - strand, within BWI76_RS00290 at 61.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 58,395 - BWI76_RS00280 0.15 +0.2 58,395 - BWI76_RS00280 0.15 +0.6 58,442 - +1.8 58,490 + BWI76_RS00280 0.25 +0.4 58,779 + BWI76_RS00280 0.55 +0.5 58,819 + BWI76_RS00280 0.59 -0.1 58,829 + BWI76_RS00280 0.60 +0.1 59,158 - -0.5 59,173 + +0.5 59,215 + +0.9 59,231 - -1.1 59,272 - -0.1 59,417 - BWI76_RS00285 0.20 +0.3 59,538 - BWI76_RS00285 0.33 +0.2 59,546 - BWI76_RS00285 0.34 +0.0 59,758 + BWI76_RS00285 0.58 +0.8 59,758 + BWI76_RS00285 0.58 +0.6 59,775 + BWI76_RS00285 0.60 +0.1 59,830 - BWI76_RS00285 0.66 +1.6 60,108 + -2.0 60,116 - +1.1 60,261 - +0.6 60,289 + +0.1 60,371 + BWI76_RS00290 0.15 +0.7 60,462 + BWI76_RS00290 0.24 +1.6 60,500 - BWI76_RS00290 0.28 -0.1 60,505 - BWI76_RS00290 0.29 +1.2 60,519 - BWI76_RS00290 0.31 +2.0 60,597 - +0.1 60,599 - BWI76_RS00290 0.39 +1.2 60,635 + BWI76_RS00290 0.43 +1.5 60,656 + BWI76_RS00290 0.45 +0.4 60,734 - BWI76_RS00290 0.54 +1.4 60,734 - BWI76_RS00290 0.54 +1.3 60,793 + BWI76_RS00290 0.60 +0.6 60,820 - BWI76_RS00290 0.63 +0.8 60,821 - BWI76_RS00290 0.63 +0.8 60,865 - BWI76_RS00290 0.68 +0.9 61,113 - +1.0
Or see this region's nucleotide sequence