Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27865 and BWI76_RS27870 overlap by 4 nucleotides BWI76_RS27870 and BWI76_RS27875 overlap by 11 nucleotides BWI76_RS27875 and BWI76_RS27880 are separated by 179 nucleotides BWI76_RS27880 and BWI76_RS27885 overlap by 4 nucleotides
BWI76_RS27865: BWI76_RS27865 - hypothetical protein, at 5,780,117 to 5,780,647
_RS27865
BWI76_RS27870: BWI76_RS27870 - membrane protein, at 5,780,644 to 5,781,006
_RS27870
BWI76_RS27875: BWI76_RS27875 - membrane protein, at 5,780,996 to 5,781,343
_RS27875
BWI76_RS27880: BWI76_RS27880 - 6-phospho-alpha-glucosidase, at 5,781,523 to 5,782,845
_RS27880
BWI76_RS27885: BWI76_RS27885 - PTS alpha-glucoside transporter subunit IICB, at 5,782,842 to 5,784,464
_RS27885
Position (kb) 5781
5782
5783 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5780.600 kb on - strand at 5780.679 kb on + strand at 5780.693 kb on - strand, within BWI76_RS27870 at 5780.775 kb on + strand, within BWI76_RS27870 at 5780.917 kb on + strand, within BWI76_RS27870 at 5780.917 kb on + strand, within BWI76_RS27870 at 5780.925 kb on - strand, within BWI76_RS27870 at 5781.046 kb on + strand, within BWI76_RS27875 at 5781.091 kb on + strand, within BWI76_RS27875 at 5781.539 kb on - strand at 5781.613 kb on - strand at 5781.697 kb on + strand, within BWI76_RS27880 at 5782.010 kb on + strand, within BWI76_RS27880 at 5782.044 kb on + strand, within BWI76_RS27880 at 5782.052 kb on - strand, within BWI76_RS27880 at 5782.053 kb on - strand, within BWI76_RS27880 at 5782.105 kb on - strand, within BWI76_RS27880 at 5782.191 kb on + strand, within BWI76_RS27880 at 5782.262 kb on - strand, within BWI76_RS27880 at 5782.295 kb on - strand, within BWI76_RS27880 at 5782.295 kb on - strand, within BWI76_RS27880 at 5782.404 kb on + strand, within BWI76_RS27880 at 5782.462 kb on + strand, within BWI76_RS27880 at 5782.643 kb on - strand, within BWI76_RS27880 at 5782.695 kb on + strand, within BWI76_RS27880 at 5782.716 kb on - strand at 5782.797 kb on + strand at 5782.797 kb on + strand at 5782.797 kb on + strand at 5782.805 kb on - strand at 5782.874 kb on - strand at 5782.900 kb on + strand at 5782.927 kb on - strand at 5782.967 kb on + strand at 5783.053 kb on + strand, within BWI76_RS27885 at 5783.053 kb on - strand, within BWI76_RS27885 at 5783.207 kb on + strand, within BWI76_RS27885 at 5783.215 kb on - strand, within BWI76_RS27885 at 5783.215 kb on - strand, within BWI76_RS27885 at 5783.393 kb on - strand, within BWI76_RS27885 at 5783.424 kb on - strand, within BWI76_RS27885 at 5783.515 kb on - strand, within BWI76_RS27885 at 5783.527 kb on - strand, within BWI76_RS27885 at 5783.527 kb on - strand, within BWI76_RS27885 at 5783.625 kb on - strand, within BWI76_RS27885 at 5783.628 kb on - strand, within BWI76_RS27885 at 5783.638 kb on - strand, within BWI76_RS27885 at 5783.745 kb on + strand, within BWI76_RS27885 at 5783.828 kb on + strand, within BWI76_RS27885
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 5,780,600 - +0.5 5,780,679 + -1.2 5,780,693 - BWI76_RS27870 0.13 +0.2 5,780,775 + BWI76_RS27870 0.36 +0.5 5,780,917 + BWI76_RS27870 0.75 +0.2 5,780,917 + BWI76_RS27870 0.75 +0.6 5,780,925 - BWI76_RS27870 0.77 -0.2 5,781,046 + BWI76_RS27875 0.14 -0.5 5,781,091 + BWI76_RS27875 0.27 +1.2 5,781,539 - -0.2 5,781,613 - +0.6 5,781,697 + BWI76_RS27880 0.13 +0.2 5,782,010 + BWI76_RS27880 0.37 +0.9 5,782,044 + BWI76_RS27880 0.39 -0.1 5,782,052 - BWI76_RS27880 0.40 +1.4 5,782,053 - BWI76_RS27880 0.40 +0.0 5,782,105 - BWI76_RS27880 0.44 +0.4 5,782,191 + BWI76_RS27880 0.50 +2.0 5,782,262 - BWI76_RS27880 0.56 +1.2 5,782,295 - BWI76_RS27880 0.58 +0.8 5,782,295 - BWI76_RS27880 0.58 +0.3 5,782,404 + BWI76_RS27880 0.67 +0.7 5,782,462 + BWI76_RS27880 0.71 +0.3 5,782,643 - BWI76_RS27880 0.85 +0.9 5,782,695 + BWI76_RS27880 0.89 +0.3 5,782,716 - +0.4 5,782,797 + -0.8 5,782,797 + +0.7 5,782,797 + +0.3 5,782,805 - +1.2 5,782,874 - -0.3 5,782,900 + +0.6 5,782,927 - +0.3 5,782,967 + +1.2 5,783,053 + BWI76_RS27885 0.13 +2.3 5,783,053 - BWI76_RS27885 0.13 -0.6 5,783,207 + BWI76_RS27885 0.22 -0.1 5,783,215 - BWI76_RS27885 0.23 +0.6 5,783,215 - BWI76_RS27885 0.23 +0.7 5,783,393 - BWI76_RS27885 0.34 +0.6 5,783,424 - BWI76_RS27885 0.36 -0.2 5,783,515 - BWI76_RS27885 0.41 -1.1 5,783,527 - BWI76_RS27885 0.42 -0.1 5,783,527 - BWI76_RS27885 0.42 +0.1 5,783,625 - BWI76_RS27885 0.48 -2.2 5,783,628 - BWI76_RS27885 0.48 +0.4 5,783,638 - BWI76_RS27885 0.49 +1.1 5,783,745 + BWI76_RS27885 0.56 +1.1 5,783,828 + BWI76_RS27885 0.61 +1.4
Or see this region's nucleotide sequence