Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27340 and BWI76_RS27345 overlap by 11 nucleotides BWI76_RS27345 and BWI76_RS27350 are separated by 4 nucleotides BWI76_RS27350 and BWI76_RS27355 are separated by 198 nucleotides BWI76_RS27355 and BWI76_RS27360 are separated by 20 nucleotides BWI76_RS27360 and BWI76_RS27365 are separated by 216 nucleotides
BWI76_RS27340: BWI76_RS27340 - hypothetical protein, at 5,675,405 to 5,676,778
_RS27340
BWI76_RS27345: BWI76_RS27345 - hypothetical protein, at 5,676,768 to 5,677,493
_RS27345
BWI76_RS27350: BWI76_RS27350 - hypothetical protein, at 5,677,498 to 5,678,175
_RS27350
BWI76_RS27355: BWI76_RS27355 - 50S ribosomal protein L33, at 5,678,374 to 5,678,541
_RS27355
BWI76_RS27360: BWI76_RS27360 - 50S ribosomal protein L28, at 5,678,562 to 5,678,798
_RS27360
BWI76_RS27365: BWI76_RS27365 - hypothetical protein, at 5,679,015 to 5,679,680
_RS27365
Position (kb) 5677
5678
5679 Strain fitness (log2 ratio) -2
-1
0
1 at 5676.513 kb on + strand, within BWI76_RS27340 at 5676.600 kb on + strand, within BWI76_RS27340 at 5676.602 kb on - strand, within BWI76_RS27340 at 5676.616 kb on + strand, within BWI76_RS27340 at 5676.785 kb on + strand at 5676.880 kb on - strand, within BWI76_RS27345 at 5676.929 kb on - strand, within BWI76_RS27345 at 5676.941 kb on + strand, within BWI76_RS27345 at 5676.941 kb on + strand, within BWI76_RS27345 at 5676.981 kb on - strand, within BWI76_RS27345 at 5677.039 kb on - strand, within BWI76_RS27345 at 5677.145 kb on + strand, within BWI76_RS27345 at 5677.249 kb on + strand, within BWI76_RS27345 at 5677.255 kb on - strand, within BWI76_RS27345 at 5677.324 kb on - strand, within BWI76_RS27345 at 5677.369 kb on - strand, within BWI76_RS27345 at 5677.553 kb on + strand at 5677.627 kb on - strand, within BWI76_RS27350 at 5677.627 kb on - strand, within BWI76_RS27350 at 5677.660 kb on - strand, within BWI76_RS27350 at 5677.739 kb on - strand, within BWI76_RS27350 at 5677.749 kb on - strand, within BWI76_RS27350 at 5677.826 kb on + strand, within BWI76_RS27350 at 5677.890 kb on + strand, within BWI76_RS27350 at 5677.964 kb on - strand, within BWI76_RS27350 at 5678.120 kb on + strand at 5678.121 kb on - strand at 5678.130 kb on + strand at 5678.321 kb on - strand at 5679.146 kb on + strand, within BWI76_RS27365 at 5679.146 kb on + strand, within BWI76_RS27365
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 5,676,513 + BWI76_RS27340 0.81 +0.3 5,676,600 + BWI76_RS27340 0.87 -0.1 5,676,602 - BWI76_RS27340 0.87 -0.2 5,676,616 + BWI76_RS27340 0.88 +0.4 5,676,785 + -0.3 5,676,880 - BWI76_RS27345 0.15 -0.2 5,676,929 - BWI76_RS27345 0.22 +0.2 5,676,941 + BWI76_RS27345 0.24 +0.5 5,676,941 + BWI76_RS27345 0.24 -0.3 5,676,981 - BWI76_RS27345 0.29 +0.1 5,677,039 - BWI76_RS27345 0.37 +0.6 5,677,145 + BWI76_RS27345 0.52 +0.1 5,677,249 + BWI76_RS27345 0.66 -0.7 5,677,255 - BWI76_RS27345 0.67 +0.9 5,677,324 - BWI76_RS27345 0.77 +0.3 5,677,369 - BWI76_RS27345 0.83 +1.1 5,677,553 + -0.9 5,677,627 - BWI76_RS27350 0.19 -0.5 5,677,627 - BWI76_RS27350 0.19 -1.9 5,677,660 - BWI76_RS27350 0.24 +1.6 5,677,739 - BWI76_RS27350 0.36 -0.5 5,677,749 - BWI76_RS27350 0.37 -0.6 5,677,826 + BWI76_RS27350 0.48 +0.0 5,677,890 + BWI76_RS27350 0.58 -1.1 5,677,964 - BWI76_RS27350 0.69 -0.8 5,678,120 + -0.3 5,678,121 - +0.1 5,678,130 + -1.1 5,678,321 - -0.3 5,679,146 + BWI76_RS27365 0.20 +0.1 5,679,146 + BWI76_RS27365 0.20 +1.6
Or see this region's nucleotide sequence