Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22275 and BWI76_RS22280 are separated by 12 nucleotides BWI76_RS22280 and BWI76_RS22285 are separated by 134 nucleotides BWI76_RS22285 and BWI76_RS22290 are separated by 256 nucleotides
BWI76_RS22275: BWI76_RS22275 - carbamoyltransferase HypF, at 4,642,677 to 4,644,896
_RS22275
BWI76_RS22280: BWI76_RS22280 - formate dehydrogenase-H ferredoxin subunit, at 4,644,909 to 4,645,436
_RS22280
BWI76_RS22285: BWI76_RS22285 - transcriptional regulator, at 4,645,571 to 4,646,584
_RS22285
BWI76_RS22290: BWI76_RS22290 - PTS cellobiose/arbutin/salicin transporter subunit IIBC, at 4,646,841 to 4,648,283
_RS22290
Position (kb)
4645
4646
4647 Strain fitness (log2 ratio)
-2
-1
0
1 at 4644.889 kb on - strand at 4644.923 kb on - strand at 4644.923 kb on - strand at 4644.961 kb on - strand at 4644.963 kb on + strand, within BWI76_RS22280 at 4645.001 kb on + strand, within BWI76_RS22280 at 4645.083 kb on - strand, within BWI76_RS22280 at 4645.202 kb on + strand, within BWI76_RS22280 at 4645.225 kb on + strand, within BWI76_RS22280 at 4645.379 kb on + strand, within BWI76_RS22280 at 4645.409 kb on - strand at 4645.770 kb on + strand, within BWI76_RS22285 at 4645.778 kb on - strand, within BWI76_RS22285 at 4645.778 kb on - strand, within BWI76_RS22285 at 4645.789 kb on + strand, within BWI76_RS22285 at 4646.340 kb on + strand, within BWI76_RS22285 at 4646.350 kb on - strand, within BWI76_RS22285 at 4646.357 kb on + strand, within BWI76_RS22285 at 4646.448 kb on - strand, within BWI76_RS22285 at 4646.573 kb on - strand at 4646.665 kb on + strand at 4646.919 kb on - strand at 4646.993 kb on - strand, within BWI76_RS22290 at 4647.091 kb on - strand, within BWI76_RS22290 at 4647.443 kb on + strand, within BWI76_RS22290 at 4647.506 kb on - strand, within BWI76_RS22290 at 4647.515 kb on + strand, within BWI76_RS22290
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 4,644,889 - -0.7 4,644,923 - +0.6 4,644,923 - -0.1 4,644,961 - -2.3 4,644,963 + BWI76_RS22280 0.10 +0.5 4,645,001 + BWI76_RS22280 0.17 +0.0 4,645,083 - BWI76_RS22280 0.33 -0.5 4,645,202 + BWI76_RS22280 0.55 -0.6 4,645,225 + BWI76_RS22280 0.60 -1.0 4,645,379 + BWI76_RS22280 0.89 -0.1 4,645,409 - -0.8 4,645,770 + BWI76_RS22285 0.20 +0.5 4,645,778 - BWI76_RS22285 0.20 +1.4 4,645,778 - BWI76_RS22285 0.20 +1.1 4,645,789 + BWI76_RS22285 0.21 +1.3 4,646,340 + BWI76_RS22285 0.76 +0.7 4,646,350 - BWI76_RS22285 0.77 +0.3 4,646,357 + BWI76_RS22285 0.78 -0.2 4,646,448 - BWI76_RS22285 0.86 +0.7 4,646,573 - +0.7 4,646,665 + +1.3 4,646,919 - -0.3 4,646,993 - BWI76_RS22290 0.11 -0.3 4,647,091 - BWI76_RS22290 0.17 +0.3 4,647,443 + BWI76_RS22290 0.42 -1.4 4,647,506 - BWI76_RS22290 0.46 +0.3 4,647,515 + BWI76_RS22290 0.47 -0.5
Or see this region's nucleotide sequence