Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22285

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22275 and BWI76_RS22280 are separated by 12 nucleotidesBWI76_RS22280 and BWI76_RS22285 are separated by 134 nucleotidesBWI76_RS22285 and BWI76_RS22290 are separated by 256 nucleotides BWI76_RS22275: BWI76_RS22275 - carbamoyltransferase HypF, at 4,642,677 to 4,644,896 _RS22275 BWI76_RS22280: BWI76_RS22280 - formate dehydrogenase-H ferredoxin subunit, at 4,644,909 to 4,645,436 _RS22280 BWI76_RS22285: BWI76_RS22285 - transcriptional regulator, at 4,645,571 to 4,646,584 _RS22285 BWI76_RS22290: BWI76_RS22290 - PTS cellobiose/arbutin/salicin transporter subunit IIBC, at 4,646,841 to 4,648,283 _RS22290 Position (kb) 4645 4646 4647Strain fitness (log2 ratio) -2 -1 0 1at 4644.889 kb on - strandat 4644.923 kb on - strandat 4644.923 kb on - strandat 4644.961 kb on - strandat 4644.963 kb on + strand, within BWI76_RS22280at 4645.001 kb on + strand, within BWI76_RS22280at 4645.083 kb on - strand, within BWI76_RS22280at 4645.202 kb on + strand, within BWI76_RS22280at 4645.225 kb on + strand, within BWI76_RS22280at 4645.379 kb on + strand, within BWI76_RS22280at 4645.409 kb on - strandat 4645.770 kb on + strand, within BWI76_RS22285at 4645.778 kb on - strand, within BWI76_RS22285at 4645.778 kb on - strand, within BWI76_RS22285at 4645.789 kb on + strand, within BWI76_RS22285at 4646.340 kb on + strand, within BWI76_RS22285at 4646.350 kb on - strand, within BWI76_RS22285at 4646.357 kb on + strand, within BWI76_RS22285at 4646.448 kb on - strand, within BWI76_RS22285at 4646.573 kb on - strandat 4646.665 kb on + strandat 4646.919 kb on - strandat 4646.993 kb on - strand, within BWI76_RS22290at 4647.091 kb on - strand, within BWI76_RS22290at 4647.443 kb on + strand, within BWI76_RS22290at 4647.506 kb on - strand, within BWI76_RS22290at 4647.515 kb on + strand, within BWI76_RS22290

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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4,644,889 - -0.7
4,644,923 - +0.6
4,644,923 - -0.1
4,644,961 - -2.3
4,644,963 + BWI76_RS22280 0.10 +0.5
4,645,001 + BWI76_RS22280 0.17 +0.0
4,645,083 - BWI76_RS22280 0.33 -0.5
4,645,202 + BWI76_RS22280 0.55 -0.6
4,645,225 + BWI76_RS22280 0.60 -1.0
4,645,379 + BWI76_RS22280 0.89 -0.1
4,645,409 - -0.8
4,645,770 + BWI76_RS22285 0.20 +0.5
4,645,778 - BWI76_RS22285 0.20 +1.4
4,645,778 - BWI76_RS22285 0.20 +1.1
4,645,789 + BWI76_RS22285 0.21 +1.3
4,646,340 + BWI76_RS22285 0.76 +0.7
4,646,350 - BWI76_RS22285 0.77 +0.3
4,646,357 + BWI76_RS22285 0.78 -0.2
4,646,448 - BWI76_RS22285 0.86 +0.7
4,646,573 - +0.7
4,646,665 + +1.3
4,646,919 - -0.3
4,646,993 - BWI76_RS22290 0.11 -0.3
4,647,091 - BWI76_RS22290 0.17 +0.3
4,647,443 + BWI76_RS22290 0.42 -1.4
4,647,506 - BWI76_RS22290 0.46 +0.3
4,647,515 + BWI76_RS22290 0.47 -0.5

Or see this region's nucleotide sequence