Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21460

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21455 and BWI76_RS21460 are separated by 271 nucleotidesBWI76_RS21460 and BWI76_RS21465 are separated by 261 nucleotides BWI76_RS21455: BWI76_RS21455 - outer membrane protein assembly factor BamD, at 4,494,985 to 4,495,722 _RS21455 BWI76_RS21460: BWI76_RS21460 - translation inhibitor protein RaiA, at 4,495,994 to 4,496,329 _RS21460 BWI76_RS21465: BWI76_RS21465 - bifunctional chorismate mutase/prephenate dehydratase, at 4,496,591 to 4,497,751 _RS21465 Position (kb) 4495 4496 4497Strain fitness (log2 ratio) -3 -2 -1 0 1at 4495.793 kb on - strandat 4495.796 kb on + strandat 4495.864 kb on + strandat 4495.864 kb on + strandat 4495.864 kb on + strandat 4495.871 kb on - strandat 4496.123 kb on - strand, within BWI76_RS21460at 4496.414 kb on + strandat 4496.535 kb on - strandat 4496.535 kb on - strandat 4497.319 kb on + strand, within BWI76_RS21465

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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4,495,793 - +0.6
4,495,796 + +0.4
4,495,864 + +0.2
4,495,864 + +0.4
4,495,864 + -0.7
4,495,871 - +1.0
4,496,123 - BWI76_RS21460 0.38 -0.8
4,496,414 + +0.9
4,496,535 - +0.4
4,496,535 - +0.0
4,497,319 + BWI76_RS21465 0.63 -3.0

Or see this region's nucleotide sequence