Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16930

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16925 and BWI76_RS16930 are separated by 190 nucleotidesBWI76_RS16930 and BWI76_RS16935 are separated by 63 nucleotidesBWI76_RS16935 and BWI76_RS16940 are separated by 31 nucleotides BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867 _RS16925 BWI76_RS16930: BWI76_RS16930 - transcriptional regulator, at 3,511,058 to 3,511,864 _RS16930 BWI76_RS16935: BWI76_RS16935 - shikimate dehydrogenase, at 3,511,928 to 3,512,764 _RS16935 BWI76_RS16940: BWI76_RS16940 - MFS transporter, at 3,512,796 to 3,514,115 _RS16940 Position (kb) 3511 3512Strain fitness (log2 ratio) -2 -1 0 1at 3510.070 kb on - strand, within BWI76_RS16925at 3510.179 kb on - strand, within BWI76_RS16925at 3510.192 kb on + strand, within BWI76_RS16925at 3510.497 kb on - strand, within BWI76_RS16925at 3510.514 kb on - strand, within BWI76_RS16925at 3510.662 kb on - strand, within BWI76_RS16925at 3510.824 kb on - strandat 3510.881 kb on + strandat 3510.966 kb on + strandat 3510.966 kb on + strandat 3511.275 kb on + strand, within BWI76_RS16930at 3511.732 kb on - strand, within BWI76_RS16930at 3511.754 kb on + strand, within BWI76_RS16930at 3511.957 kb on + strandat 3512.130 kb on - strand, within BWI76_RS16935at 3512.708 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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3,510,070 - BWI76_RS16925 0.52 +1.1
3,510,179 - BWI76_RS16925 0.59 +0.0
3,510,192 + BWI76_RS16925 0.59 +0.3
3,510,497 - BWI76_RS16925 0.78 -1.3
3,510,514 - BWI76_RS16925 0.79 +0.1
3,510,662 - BWI76_RS16925 0.88 -1.1
3,510,824 - -0.2
3,510,881 + -1.0
3,510,966 + +0.5
3,510,966 + +0.3
3,511,275 + BWI76_RS16930 0.27 -0.4
3,511,732 - BWI76_RS16930 0.84 -1.8
3,511,754 + BWI76_RS16930 0.86 -0.0
3,511,957 + -1.0
3,512,130 - BWI76_RS16935 0.24 +0.3
3,512,708 - +0.7

Or see this region's nucleotide sequence