Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15930

Experiment: K. M5a1 KLEB22 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15925 and BWI76_RS15930 are separated by 10 nucleotidesBWI76_RS15930 and BWI76_RS15935 are separated by 1 nucleotidesBWI76_RS15935 and BWI76_RS15940 are separated by 41 nucleotides BWI76_RS15925: BWI76_RS15925 - dimethyl sulfoxide reductase subunit A, at 3,302,259 to 3,304,694 _RS15925 BWI76_RS15930: BWI76_RS15930 - dimethylsulfoxide reductase, chain B, at 3,304,705 to 3,305,322 _RS15930 BWI76_RS15935: BWI76_RS15935 - dimethyl sulfoxide reductase, at 3,305,324 to 3,306,181 _RS15935 BWI76_RS15940: BWI76_RS15940 - DMSO reductase maturation protein DsmD, at 3,306,223 to 3,306,831 _RS15940 Position (kb) 3304 3305 3306Strain fitness (log2 ratio) -2 -1 0 1at 3303.716 kb on + strand, within BWI76_RS15925at 3303.748 kb on - strand, within BWI76_RS15925at 3304.008 kb on - strand, within BWI76_RS15925at 3304.663 kb on + strandat 3304.928 kb on + strand, within BWI76_RS15930at 3304.928 kb on + strand, within BWI76_RS15930at 3304.936 kb on - strand, within BWI76_RS15930at 3304.936 kb on - strand, within BWI76_RS15930at 3305.042 kb on + strand, within BWI76_RS15930at 3305.723 kb on + strand, within BWI76_RS15935

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1
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3,303,716 + BWI76_RS15925 0.60 -1.9
3,303,748 - BWI76_RS15925 0.61 +0.1
3,304,008 - BWI76_RS15925 0.72 -0.4
3,304,663 + +0.2
3,304,928 + BWI76_RS15930 0.36 +0.2
3,304,928 + BWI76_RS15930 0.36 -0.9
3,304,936 - BWI76_RS15930 0.37 -1.5
3,304,936 - BWI76_RS15930 0.37 -1.5
3,305,042 + BWI76_RS15930 0.55 -0.9
3,305,723 + BWI76_RS15935 0.47 +0.4

Or see this region's nucleotide sequence