Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15765 and BWI76_RS15770 overlap by 4 nucleotides BWI76_RS15770 and BWI76_RS15775 overlap by 4 nucleotides BWI76_RS15775 and BWI76_RS15780 are separated by 48 nucleotides
BWI76_RS15760: BWI76_RS15760 - hypothetical protein, at 3,282,708 to 3,282,899
_RS15760
BWI76_RS15765: BWI76_RS15765 - hypothetical protein, at 3,282,883 to 3,283,599
_RS15765
BWI76_RS15770: BWI76_RS15770 - hypothetical protein, at 3,283,596 to 3,283,901
_RS15770
BWI76_RS15775: BWI76_RS15775 - replication protein, at 3,283,898 to 3,284,224
_RS15775
BWI76_RS15780: BWI76_RS15780 - hypothetical protein, at 3,284,273 to 3,285,513
_RS15780
Position (kb) 3283
3284
3285 Strain fitness (log2 ratio) -1
0
1
2 at 3282.928 kb on - strand at 3283.036 kb on - strand, within BWI76_RS15765 at 3283.144 kb on + strand, within BWI76_RS15765 at 3283.201 kb on - strand, within BWI76_RS15765 at 3283.292 kb on + strand, within BWI76_RS15765 at 3283.733 kb on - strand, within BWI76_RS15770 at 3283.740 kb on - strand, within BWI76_RS15770 at 3283.773 kb on - strand, within BWI76_RS15770 at 3283.804 kb on - strand, within BWI76_RS15770 at 3283.965 kb on - strand, within BWI76_RS15775
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 3,282,928 - +1.7 3,283,036 - BWI76_RS15765 0.21 +0.1 3,283,144 + BWI76_RS15765 0.36 +0.9 3,283,201 - BWI76_RS15765 0.44 +2.4 3,283,292 + BWI76_RS15765 0.57 -0.6 3,283,733 - BWI76_RS15770 0.45 -0.6 3,283,740 - BWI76_RS15770 0.47 -0.5 3,283,773 - BWI76_RS15770 0.58 +0.2 3,283,804 - BWI76_RS15770 0.68 -0.4 3,283,965 - BWI76_RS15775 0.20 -0.7
Or see this region's nucleotide sequence