Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10740 and BWI76_RS10745 are separated by 374 nucleotides BWI76_RS10745 and BWI76_RS10750 are separated by 258 nucleotides BWI76_RS10750 and BWI76_RS10755 are separated by 21 nucleotides BWI76_RS10755 and BWI76_RS10760 are separated by 9 nucleotides
BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701
_RS10740
BWI76_RS10745: BWI76_RS10745 - hypothetical protein, at 2,270,076 to 2,270,249
_RS10745
BWI76_RS10750: BWI76_RS10750 - pyrimidine utilization transport protein G, at 2,270,508 to 2,271,830
_RS10750
BWI76_RS10755: BWI76_RS10755 - pyrimidine utilization flavin reductase protein F, at 2,271,852 to 2,272,346
_RS10755
BWI76_RS10760: BWI76_RS10760 - nitroreductase family protein, at 2,272,356 to 2,272,946
_RS10760
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-2
-1
0
1 at 2269.610 kb on - strand, within BWI76_RS10740 at 2269.634 kb on - strand, within BWI76_RS10740 at 2269.652 kb on + strand at 2269.881 kb on - strand at 2269.988 kb on - strand at 2270.016 kb on + strand at 2270.024 kb on - strand at 2270.035 kb on + strand at 2270.159 kb on - strand, within BWI76_RS10745 at 2270.275 kb on + strand at 2270.282 kb on - strand at 2270.551 kb on + strand at 2270.668 kb on + strand, within BWI76_RS10750 at 2270.714 kb on - strand, within BWI76_RS10750 at 2270.872 kb on - strand, within BWI76_RS10750 at 2271.104 kb on - strand, within BWI76_RS10750 at 2271.359 kb on + strand, within BWI76_RS10750 at 2271.479 kb on + strand, within BWI76_RS10750 at 2271.499 kb on + strand, within BWI76_RS10750 at 2271.507 kb on - strand, within BWI76_RS10750 at 2271.852 kb on - strand at 2271.911 kb on + strand, within BWI76_RS10755 at 2272.362 kb on - strand at 2272.685 kb on - strand, within BWI76_RS10760 at 2272.734 kb on + strand, within BWI76_RS10760 at 2272.741 kb on + strand, within BWI76_RS10760 at 2272.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 2,269,610 - BWI76_RS10740 0.85 -0.5 2,269,634 - BWI76_RS10740 0.89 -0.3 2,269,652 + -0.3 2,269,881 - -0.2 2,269,988 - +0.1 2,270,016 + -0.0 2,270,024 - -0.1 2,270,035 + +0.4 2,270,159 - BWI76_RS10745 0.48 +0.2 2,270,275 + +0.4 2,270,282 - +0.1 2,270,551 + -0.4 2,270,668 + BWI76_RS10750 0.12 -0.4 2,270,714 - BWI76_RS10750 0.16 +1.1 2,270,872 - BWI76_RS10750 0.28 -1.7 2,271,104 - BWI76_RS10750 0.45 -1.9 2,271,359 + BWI76_RS10750 0.64 -1.8 2,271,479 + BWI76_RS10750 0.73 +0.9 2,271,499 + BWI76_RS10750 0.75 -0.9 2,271,507 - BWI76_RS10750 0.76 -0.9 2,271,852 - -0.8 2,271,911 + BWI76_RS10755 0.12 -0.1 2,272,362 - -0.5 2,272,685 - BWI76_RS10760 0.56 -0.7 2,272,734 + BWI76_RS10760 0.64 +0.3 2,272,741 + BWI76_RS10760 0.65 +0.0 2,272,742 - -0.8
Or see this region's nucleotide sequence