Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09740 and BWI76_RS09745 are separated by 382 nucleotides BWI76_RS09745 and BWI76_RS09750 are separated by 114 nucleotides BWI76_RS09750 and BWI76_RS09755 are separated by 186 nucleotides
BWI76_RS09740: BWI76_RS09740 - formate transporter FocA, at 2,042,161 to 2,043,018
_RS09740
BWI76_RS09745: BWI76_RS09745 - 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO, at 2,043,401 to 2,045,161
_RS09745
BWI76_RS09750: BWI76_RS09750 - DUF421 domain-containing protein, at 2,045,276 to 2,045,968
_RS09750
BWI76_RS09755: BWI76_RS09755 - phosphoserine transaminase, at 2,046,155 to 2,047,243
_RS09755
Position (kb)
2043
2044
2045
2046 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2042.484 kb on + strand, within BWI76_RS09740 at 2042.584 kb on + strand, within BWI76_RS09740 at 2042.672 kb on - strand, within BWI76_RS09740 at 2042.754 kb on + strand, within BWI76_RS09740 at 2042.864 kb on - strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.900 kb on - strand, within BWI76_RS09740 at 2042.902 kb on - strand, within BWI76_RS09740 at 2042.902 kb on - strand, within BWI76_RS09740 at 2042.910 kb on + strand, within BWI76_RS09740 at 2042.976 kb on + strand at 2042.978 kb on + strand at 2043.004 kb on + strand at 2043.018 kb on + strand at 2043.026 kb on - strand at 2043.028 kb on + strand at 2043.030 kb on - strand at 2043.030 kb on - strand at 2043.063 kb on + strand at 2043.063 kb on + strand at 2043.071 kb on - strand at 2043.071 kb on - strand at 2043.071 kb on - strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.097 kb on - strand at 2043.097 kb on - strand at 2043.121 kb on - strand at 2043.345 kb on + strand at 2043.447 kb on + strand at 2043.455 kb on - strand at 2043.530 kb on - strand at 2043.613 kb on - strand, within BWI76_RS09745 at 2043.635 kb on + strand, within BWI76_RS09745 at 2043.689 kb on + strand, within BWI76_RS09745 at 2043.711 kb on - strand, within BWI76_RS09745 at 2043.716 kb on - strand, within BWI76_RS09745 at 2043.778 kb on + strand, within BWI76_RS09745 at 2043.786 kb on - strand, within BWI76_RS09745 at 2044.025 kb on + strand, within BWI76_RS09745 at 2044.097 kb on + strand, within BWI76_RS09745 at 2044.234 kb on + strand, within BWI76_RS09745 at 2044.386 kb on - strand, within BWI76_RS09745 at 2044.549 kb on - strand, within BWI76_RS09745 at 2045.018 kb on + strand at 2045.018 kb on + strand at 2045.026 kb on - strand at 2045.041 kb on + strand at 2045.041 kb on + strand at 2045.259 kb on - strand at 2045.259 kb on - strand at 2045.337 kb on + strand at 2045.347 kb on + strand, within BWI76_RS09750 at 2045.354 kb on + strand, within BWI76_RS09750 at 2045.361 kb on - strand, within BWI76_RS09750 at 2045.433 kb on - strand, within BWI76_RS09750 at 2045.490 kb on + strand, within BWI76_RS09750 at 2045.778 kb on - strand, within BWI76_RS09750 at 2045.796 kb on + strand, within BWI76_RS09750 at 2045.934 kb on + strand at 2045.999 kb on - strand at 2046.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 2,042,484 + BWI76_RS09740 0.38 +1.1 2,042,584 + BWI76_RS09740 0.49 +2.8 2,042,672 - BWI76_RS09740 0.60 -0.2 2,042,754 + BWI76_RS09740 0.69 +1.6 2,042,864 - BWI76_RS09740 0.82 +0.6 2,042,884 + BWI76_RS09740 0.84 -0.6 2,042,884 + BWI76_RS09740 0.84 +1.2 2,042,884 + BWI76_RS09740 0.84 -0.5 2,042,884 + BWI76_RS09740 0.84 +0.4 2,042,892 - BWI76_RS09740 0.85 +0.5 2,042,892 - BWI76_RS09740 0.85 +1.3 2,042,892 - BWI76_RS09740 0.85 +0.2 2,042,892 - BWI76_RS09740 0.85 +0.0 2,042,892 - BWI76_RS09740 0.85 -1.7 2,042,900 - BWI76_RS09740 0.86 +1.5 2,042,902 - BWI76_RS09740 0.86 +0.9 2,042,902 - BWI76_RS09740 0.86 +0.1 2,042,910 + BWI76_RS09740 0.87 +1.2 2,042,976 + -0.0 2,042,978 + -1.6 2,043,004 + -0.4 2,043,018 + +0.5 2,043,026 - -1.3 2,043,028 + -1.1 2,043,030 - +0.4 2,043,030 - +0.8 2,043,063 + +1.7 2,043,063 + -0.5 2,043,071 - +0.5 2,043,071 - +0.8 2,043,071 - +0.7 2,043,088 + +1.0 2,043,088 + +0.9 2,043,088 + -0.5 2,043,088 + +1.0 2,043,088 + +0.7 2,043,088 + +1.1 2,043,088 + +1.7 2,043,088 + +0.4 2,043,088 + +0.3 2,043,088 + +0.9 2,043,096 - +1.6 2,043,096 - +2.9 2,043,096 - +1.1 2,043,096 - +1.1 2,043,096 - +0.9 2,043,097 - +1.6 2,043,097 - +0.8 2,043,121 - +1.9 2,043,345 + +1.0 2,043,447 + +0.7 2,043,455 - +0.6 2,043,530 - +0.8 2,043,613 - BWI76_RS09745 0.12 +1.1 2,043,635 + BWI76_RS09745 0.13 +0.6 2,043,689 + BWI76_RS09745 0.16 +0.5 2,043,711 - BWI76_RS09745 0.18 +0.7 2,043,716 - BWI76_RS09745 0.18 +1.7 2,043,778 + BWI76_RS09745 0.21 +1.7 2,043,786 - BWI76_RS09745 0.22 +1.1 2,044,025 + BWI76_RS09745 0.35 +0.4 2,044,097 + BWI76_RS09745 0.40 +1.2 2,044,234 + BWI76_RS09745 0.47 -0.1 2,044,386 - BWI76_RS09745 0.56 +0.3 2,044,549 - BWI76_RS09745 0.65 +1.4 2,045,018 + +0.3 2,045,018 + +1.0 2,045,026 - +0.6 2,045,041 + +0.6 2,045,041 + +0.8 2,045,259 - +0.9 2,045,259 - -1.2 2,045,337 + +0.3 2,045,347 + BWI76_RS09750 0.10 +0.6 2,045,354 + BWI76_RS09750 0.11 +0.3 2,045,361 - BWI76_RS09750 0.12 +0.7 2,045,433 - BWI76_RS09750 0.23 +0.1 2,045,490 + BWI76_RS09750 0.31 +0.2 2,045,778 - BWI76_RS09750 0.72 +0.1 2,045,796 + BWI76_RS09750 0.75 +0.6 2,045,934 + +0.5 2,045,999 - +0.8 2,046,054 - -0.5
Or see this region's nucleotide sequence