Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09115 and BWI76_RS09120 are separated by 259 nucleotides BWI76_RS09120 and BWI76_RS09125 are separated by 33 nucleotides BWI76_RS09125 and BWI76_RS09130 are separated by 376 nucleotides BWI76_RS09130 and BWI76_RS09135 are separated by 104 nucleotides
BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040
_RS09115
BWI76_RS09120: BWI76_RS09120 - hypothetical protein, at 1,927,300 to 1,927,818
_RS09120
BWI76_RS09125: BWI76_RS09125 - MFS transporter, at 1,927,852 to 1,929,096
_RS09125
BWI76_RS09130: BWI76_RS09130 - biofilm formation regulatory protein BssR, at 1,929,473 to 1,929,856
_RS09130
BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073
_RS09135
Position (kb)
1927
1928
1929
1930 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1926.855 kb on + strand, within BWI76_RS09115 at 1927.001 kb on - strand at 1927.069 kb on + strand at 1927.077 kb on - strand at 1927.082 kb on - strand at 1927.111 kb on - strand at 1927.113 kb on + strand at 1927.228 kb on + strand at 1927.228 kb on + strand at 1927.228 kb on + strand at 1927.236 kb on - strand at 1927.236 kb on - strand at 1927.238 kb on + strand at 1927.238 kb on + strand at 1927.241 kb on - strand at 1927.246 kb on - strand at 1927.318 kb on + strand at 1927.333 kb on - strand at 1927.343 kb on - strand at 1927.383 kb on - strand at 1927.386 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.498 kb on - strand, within BWI76_RS09120 at 1927.509 kb on + strand, within BWI76_RS09120 at 1927.517 kb on - strand, within BWI76_RS09120 at 1927.517 kb on - strand, within BWI76_RS09120 at 1927.542 kb on + strand, within BWI76_RS09120 at 1927.548 kb on + strand, within BWI76_RS09120 at 1927.548 kb on + strand, within BWI76_RS09120 at 1927.596 kb on + strand, within BWI76_RS09120 at 1927.604 kb on - strand, within BWI76_RS09120 at 1927.606 kb on + strand, within BWI76_RS09120 at 1927.675 kb on - strand, within BWI76_RS09120 at 1927.796 kb on + strand at 1927.866 kb on - strand at 1927.881 kb on + strand at 1927.902 kb on + strand at 1927.928 kb on + strand at 1927.945 kb on + strand at 1927.945 kb on + strand at 1927.973 kb on - strand at 1928.318 kb on - strand, within BWI76_RS09125 at 1928.372 kb on + strand, within BWI76_RS09125 at 1928.469 kb on + strand, within BWI76_RS09125 at 1928.469 kb on + strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.541 kb on + strand, within BWI76_RS09125 at 1928.541 kb on + strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.591 kb on - strand, within BWI76_RS09125 at 1928.765 kb on - strand, within BWI76_RS09125 at 1928.799 kb on - strand, within BWI76_RS09125 at 1928.845 kb on - strand, within BWI76_RS09125 at 1928.948 kb on + strand, within BWI76_RS09125 at 1928.953 kb on + strand, within BWI76_RS09125 at 1929.039 kb on - strand at 1929.105 kb on + strand at 1929.126 kb on - strand at 1929.476 kb on + strand at 1929.495 kb on - strand at 1929.619 kb on + strand, within BWI76_RS09130 at 1929.740 kb on + strand, within BWI76_RS09130 at 1929.748 kb on - strand, within BWI76_RS09130 at 1929.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 1,926,855 + BWI76_RS09115 0.86 -0.7 1,927,001 - +0.1 1,927,069 + -0.1 1,927,077 - +0.8 1,927,082 - -1.0 1,927,111 - +0.3 1,927,113 + -0.1 1,927,228 + +2.2 1,927,228 + -0.6 1,927,228 + -0.4 1,927,236 - -1.5 1,927,236 - -0.1 1,927,238 + +0.8 1,927,238 + +0.7 1,927,241 - -0.1 1,927,246 - +0.8 1,927,318 + -0.6 1,927,333 - +1.3 1,927,343 - -1.4 1,927,383 - -1.5 1,927,386 + BWI76_RS09120 0.17 -0.3 1,927,472 + BWI76_RS09120 0.33 +1.1 1,927,472 + BWI76_RS09120 0.33 -0.2 1,927,472 + BWI76_RS09120 0.33 +1.0 1,927,498 - BWI76_RS09120 0.38 +0.7 1,927,509 + BWI76_RS09120 0.40 -0.4 1,927,517 - BWI76_RS09120 0.42 -0.2 1,927,517 - BWI76_RS09120 0.42 -1.4 1,927,542 + BWI76_RS09120 0.47 +0.4 1,927,548 + BWI76_RS09120 0.48 +0.3 1,927,548 + BWI76_RS09120 0.48 -0.1 1,927,596 + BWI76_RS09120 0.57 -2.0 1,927,604 - BWI76_RS09120 0.59 +1.0 1,927,606 + BWI76_RS09120 0.59 +0.5 1,927,675 - BWI76_RS09120 0.72 -0.6 1,927,796 + +0.5 1,927,866 - -0.4 1,927,881 + +0.5 1,927,902 + -0.1 1,927,928 + +0.7 1,927,945 + -0.6 1,927,945 + +0.4 1,927,973 - -0.3 1,928,318 - BWI76_RS09125 0.37 +0.1 1,928,372 + BWI76_RS09125 0.42 -0.6 1,928,469 + BWI76_RS09125 0.50 -0.5 1,928,469 + BWI76_RS09125 0.50 -0.2 1,928,477 - BWI76_RS09125 0.50 +0.3 1,928,477 - BWI76_RS09125 0.50 +0.2 1,928,477 - BWI76_RS09125 0.50 -0.6 1,928,541 + BWI76_RS09125 0.55 +1.1 1,928,541 + BWI76_RS09125 0.55 -1.3 1,928,549 - BWI76_RS09125 0.56 +0.5 1,928,549 - BWI76_RS09125 0.56 +0.1 1,928,549 - BWI76_RS09125 0.56 +0.2 1,928,591 - BWI76_RS09125 0.59 +0.0 1,928,765 - BWI76_RS09125 0.73 +0.4 1,928,799 - BWI76_RS09125 0.76 +1.5 1,928,845 - BWI76_RS09125 0.80 +0.1 1,928,948 + BWI76_RS09125 0.88 -0.4 1,928,953 + BWI76_RS09125 0.88 -0.2 1,929,039 - -0.2 1,929,105 + -1.3 1,929,126 - +0.3 1,929,476 + -0.2 1,929,495 - -1.3 1,929,619 + BWI76_RS09130 0.38 -0.8 1,929,740 + BWI76_RS09130 0.70 -0.4 1,929,748 - BWI76_RS09130 0.72 +0.4 1,929,929 - -0.6
Or see this region's nucleotide sequence