Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03810 and BWI76_RS03815 are separated by 11 nucleotides BWI76_RS03815 and BWI76_RS03820 are separated by 109 nucleotides BWI76_RS03820 and BWI76_RS03825 are separated by 295 nucleotides BWI76_RS03825 and BWI76_RS03830 are separated by 19 nucleotides
BWI76_RS03810: BWI76_RS03810 - GTPase, at 787,632 to 788,588
_RS03810
BWI76_RS03815: BWI76_RS03815 - hypothetical protein, at 788,600 to 788,803
_RS03815
BWI76_RS03820: BWI76_RS03820 - carbon starvation protein A, at 788,913 to 791,063
_RS03820
BWI76_RS03825: BWI76_RS03825 - 4-hydroxyphenylacetate 3-monooxygenase, at 791,359 to 791,871
_RS03825
BWI76_RS03830: BWI76_RS03830 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 791,891 to 793,453
_RS03830
Position (kb) 788
789
790
791
792 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 788.073 kb on - strand, within BWI76_RS03810 at 788.075 kb on - strand, within BWI76_RS03810 at 788.199 kb on + strand, within BWI76_RS03810 at 788.286 kb on - strand, within BWI76_RS03810 at 788.320 kb on - strand, within BWI76_RS03810 at 788.365 kb on + strand, within BWI76_RS03810 at 788.413 kb on + strand, within BWI76_RS03810 at 788.421 kb on - strand, within BWI76_RS03810 at 788.423 kb on + strand, within BWI76_RS03810 at 788.524 kb on - strand at 788.590 kb on - strand at 788.697 kb on + strand, within BWI76_RS03815 at 788.707 kb on + strand, within BWI76_RS03815 at 788.831 kb on + strand at 788.844 kb on + strand at 788.852 kb on - strand at 788.891 kb on - strand at 788.992 kb on - strand at 789.068 kb on - strand at 789.076 kb on + strand at 789.199 kb on + strand, within BWI76_RS03820 at 789.207 kb on - strand, within BWI76_RS03820 at 789.207 kb on - strand, within BWI76_RS03820 at 789.207 kb on - strand, within BWI76_RS03820 at 789.240 kb on - strand, within BWI76_RS03820 at 789.302 kb on + strand, within BWI76_RS03820 at 789.310 kb on - strand, within BWI76_RS03820 at 789.310 kb on - strand, within BWI76_RS03820 at 789.310 kb on - strand, within BWI76_RS03820 at 789.518 kb on + strand, within BWI76_RS03820 at 789.681 kb on + strand, within BWI76_RS03820 at 789.689 kb on - strand, within BWI76_RS03820 at 790.103 kb on + strand, within BWI76_RS03820 at 790.114 kb on + strand, within BWI76_RS03820 at 790.159 kb on - strand, within BWI76_RS03820 at 790.242 kb on + strand, within BWI76_RS03820 at 790.280 kb on + strand, within BWI76_RS03820 at 790.287 kb on + strand, within BWI76_RS03820 at 790.287 kb on + strand, within BWI76_RS03820 at 790.288 kb on - strand, within BWI76_RS03820 at 790.288 kb on - strand, within BWI76_RS03820 at 790.301 kb on + strand, within BWI76_RS03820 at 790.316 kb on - strand, within BWI76_RS03820 at 790.438 kb on + strand, within BWI76_RS03820 at 790.446 kb on - strand, within BWI76_RS03820 at 790.480 kb on - strand, within BWI76_RS03820 at 790.662 kb on + strand, within BWI76_RS03820 at 790.662 kb on + strand, within BWI76_RS03820 at 790.723 kb on - strand, within BWI76_RS03820 at 790.829 kb on - strand, within BWI76_RS03820 at 790.829 kb on - strand, within BWI76_RS03820 at 790.849 kb on + strand at 790.872 kb on - strand at 790.962 kb on + strand at 790.972 kb on + strand at 791.031 kb on + strand at 791.039 kb on - strand at 791.129 kb on - strand at 791.234 kb on + strand at 791.442 kb on - strand, within BWI76_RS03825 at 791.475 kb on - strand, within BWI76_RS03825 at 791.493 kb on - strand, within BWI76_RS03825 at 791.504 kb on - strand at 791.530 kb on + strand, within BWI76_RS03825 at 791.652 kb on - strand, within BWI76_RS03825 at 791.652 kb on - strand, within BWI76_RS03825 at 791.835 kb on - strand at 791.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 788,073 - BWI76_RS03810 0.46 -0.8 788,075 - BWI76_RS03810 0.46 +0.3 788,199 + BWI76_RS03810 0.59 +0.5 788,286 - BWI76_RS03810 0.68 +0.7 788,320 - BWI76_RS03810 0.72 +0.7 788,365 + BWI76_RS03810 0.77 +0.8 788,413 + BWI76_RS03810 0.82 +1.0 788,421 - BWI76_RS03810 0.82 +0.7 788,423 + BWI76_RS03810 0.83 -0.5 788,524 - +0.7 788,590 - +0.6 788,697 + BWI76_RS03815 0.48 +0.6 788,707 + BWI76_RS03815 0.52 +1.4 788,831 + +0.7 788,844 + +0.8 788,852 - +0.0 788,891 - -0.2 788,992 - +0.1 789,068 - +0.1 789,076 + +0.5 789,199 + BWI76_RS03820 0.13 +1.4 789,207 - BWI76_RS03820 0.14 -0.9 789,207 - BWI76_RS03820 0.14 +0.9 789,207 - BWI76_RS03820 0.14 +1.1 789,240 - BWI76_RS03820 0.15 +0.3 789,302 + BWI76_RS03820 0.18 +0.9 789,310 - BWI76_RS03820 0.18 +0.5 789,310 - BWI76_RS03820 0.18 +0.4 789,310 - BWI76_RS03820 0.18 +0.2 789,518 + BWI76_RS03820 0.28 +2.9 789,681 + BWI76_RS03820 0.36 +1.6 789,689 - BWI76_RS03820 0.36 +0.9 790,103 + BWI76_RS03820 0.55 +2.2 790,114 + BWI76_RS03820 0.56 +0.5 790,159 - BWI76_RS03820 0.58 +0.1 790,242 + BWI76_RS03820 0.62 +0.6 790,280 + BWI76_RS03820 0.64 +0.6 790,287 + BWI76_RS03820 0.64 +0.2 790,287 + BWI76_RS03820 0.64 +1.2 790,288 - BWI76_RS03820 0.64 +0.2 790,288 - BWI76_RS03820 0.64 -0.5 790,301 + BWI76_RS03820 0.65 +1.3 790,316 - BWI76_RS03820 0.65 +1.0 790,438 + BWI76_RS03820 0.71 +0.5 790,446 - BWI76_RS03820 0.71 -1.4 790,480 - BWI76_RS03820 0.73 +0.3 790,662 + BWI76_RS03820 0.81 +0.3 790,662 + BWI76_RS03820 0.81 -0.0 790,723 - BWI76_RS03820 0.84 -0.3 790,829 - BWI76_RS03820 0.89 -0.5 790,829 - BWI76_RS03820 0.89 +0.1 790,849 + -0.4 790,872 - -0.0 790,962 + +1.5 790,972 + +2.1 791,031 + -0.4 791,039 - -0.4 791,129 - +0.2 791,234 + -0.5 791,442 - BWI76_RS03825 0.16 +0.7 791,475 - BWI76_RS03825 0.23 +1.4 791,493 - BWI76_RS03825 0.26 +0.9 791,504 - -0.1 791,530 + BWI76_RS03825 0.33 +1.7 791,652 - BWI76_RS03825 0.57 -0.3 791,652 - BWI76_RS03825 0.57 +0.7 791,835 - +0.6 791,854 + -1.8
Or see this region's nucleotide sequence