Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02965 and BWI76_RS02970 are separated by 131 nucleotides BWI76_RS02970 and BWI76_RS02975 are separated by 37 nucleotides BWI76_RS02975 and BWI76_RS02980 are separated by 145 nucleotides
BWI76_RS02965: BWI76_RS02965 - PTS trehalose transporter subunit IIBC, at 607,360 to 608,778
_RS02965
BWI76_RS02970: BWI76_RS02970 - trehalose operon repressor, at 608,910 to 609,857
_RS02970
BWI76_RS02975: BWI76_RS02975 - hypothetical protein, at 609,895 to 610,091
_RS02975
BWI76_RS02980: BWI76_RS02980 - type I magnesium transporting ATPase, at 610,237 to 612,945
_RS02980
Position (kb) 608
609
610 Strain fitness (log2 ratio) -1
0
1
2 at 607.912 kb on + strand, within BWI76_RS02965 at 607.915 kb on - strand, within BWI76_RS02965 at 608.178 kb on + strand, within BWI76_RS02965 at 608.408 kb on + strand, within BWI76_RS02965 at 608.534 kb on + strand, within BWI76_RS02965 at 608.549 kb on + strand at 608.576 kb on - strand, within BWI76_RS02965 at 608.585 kb on + strand, within BWI76_RS02965 at 608.585 kb on + strand, within BWI76_RS02965 at 608.698 kb on - strand at 608.792 kb on - strand at 608.945 kb on + strand at 609.101 kb on + strand, within BWI76_RS02970 at 609.396 kb on - strand, within BWI76_RS02970 at 609.488 kb on + strand, within BWI76_RS02970 at 609.617 kb on - strand, within BWI76_RS02970 at 609.668 kb on - strand, within BWI76_RS02970 at 609.888 kb on - strand at 609.890 kb on + strand at 609.898 kb on - strand at 610.134 kb on + strand at 610.197 kb on - strand at 610.303 kb on - strand at 610.567 kb on + strand, within BWI76_RS02980 at 610.852 kb on - strand, within BWI76_RS02980
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 607,912 + BWI76_RS02965 0.39 +0.4 607,915 - BWI76_RS02965 0.39 +0.3 608,178 + BWI76_RS02965 0.58 -0.0 608,408 + BWI76_RS02965 0.74 -0.2 608,534 + BWI76_RS02965 0.83 +1.2 608,549 + +2.7 608,576 - BWI76_RS02965 0.86 +0.5 608,585 + BWI76_RS02965 0.86 +0.7 608,585 + BWI76_RS02965 0.86 +0.7 608,698 - -0.5 608,792 - +2.0 608,945 + +0.9 609,101 + BWI76_RS02970 0.20 +1.0 609,396 - BWI76_RS02970 0.51 -0.9 609,488 + BWI76_RS02970 0.61 +0.3 609,617 - BWI76_RS02970 0.75 +0.5 609,668 - BWI76_RS02970 0.80 +1.1 609,888 - -0.1 609,890 + +1.0 609,898 - -0.8 610,134 + +1.3 610,197 - +0.3 610,303 - +0.3 610,567 + BWI76_RS02980 0.12 +0.3 610,852 - BWI76_RS02980 0.23 +0.5
Or see this region's nucleotide sequence