Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00100 and BWI76_RS00105 are separated by 37 nucleotides BWI76_RS00105 and BWI76_RS00110 are separated by 48 nucleotides BWI76_RS00110 and BWI76_RS00115 are separated by 164 nucleotides BWI76_RS00115 and BWI76_RS00120 are separated by 41 nucleotides
BWI76_RS00100: BWI76_RS00100 - hypothetical protein, at 19,469 to 20,206
_RS00100
BWI76_RS00105: BWI76_RS00105 - NAD(P)H-dependent oxidoreductase, at 20,244 to 20,810
_RS00105
BWI76_RS00110: BWI76_RS00110 - adenine permease PurP, at 20,859 to 22,199
_RS00110
BWI76_RS00115: BWI76_RS00115 - 6-phosphogluconate phosphatase, at 22,364 to 23,029
_RS00115
BWI76_RS00120: BWI76_RS00120 - glucosamine-6-phosphate deaminase, at 23,071 to 23,808
_RS00120
Position (kb)
20
21
22
23 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 19.967 kb on + strand, within BWI76_RS00100 at 20.030 kb on + strand, within BWI76_RS00100 at 20.038 kb on - strand, within BWI76_RS00100 at 20.053 kb on - strand, within BWI76_RS00100 at 20.123 kb on + strand, within BWI76_RS00100 at 20.244 kb on - strand at 20.281 kb on - strand at 20.314 kb on + strand, within BWI76_RS00105 at 20.322 kb on - strand, within BWI76_RS00105 at 20.350 kb on - strand, within BWI76_RS00105 at 20.436 kb on - strand, within BWI76_RS00105 at 20.467 kb on - strand, within BWI76_RS00105 at 20.495 kb on - strand, within BWI76_RS00105 at 20.502 kb on + strand, within BWI76_RS00105 at 20.588 kb on - strand, within BWI76_RS00105 at 20.715 kb on - strand, within BWI76_RS00105 at 20.806 kb on - strand at 20.835 kb on - strand at 20.856 kb on + strand at 20.856 kb on + strand at 20.864 kb on - strand at 20.929 kb on - strand at 20.929 kb on - strand at 20.989 kb on - strand at 21.080 kb on + strand, within BWI76_RS00110 at 21.359 kb on - strand, within BWI76_RS00110 at 21.459 kb on + strand, within BWI76_RS00110 at 21.538 kb on + strand, within BWI76_RS00110 at 21.578 kb on - strand, within BWI76_RS00110 at 21.737 kb on + strand, within BWI76_RS00110 at 21.957 kb on - strand, within BWI76_RS00110 at 22.121 kb on - strand at 22.125 kb on - strand at 22.130 kb on - strand at 22.416 kb on - strand at 22.506 kb on - strand, within BWI76_RS00115 at 22.559 kb on - strand, within BWI76_RS00115 at 22.675 kb on - strand at 22.686 kb on + strand, within BWI76_RS00115 at 22.694 kb on - strand, within BWI76_RS00115 at 22.854 kb on - strand, within BWI76_RS00115 at 22.890 kb on - strand, within BWI76_RS00115 at 23.039 kb on + strand at 23.076 kb on - strand at 23.081 kb on - strand at 23.133 kb on + strand at 23.170 kb on - strand, within BWI76_RS00120
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 19,967 + BWI76_RS00100 0.67 +0.3 20,030 + BWI76_RS00100 0.76 +0.2 20,038 - BWI76_RS00100 0.77 +0.5 20,053 - BWI76_RS00100 0.79 +0.7 20,123 + BWI76_RS00100 0.89 +0.4 20,244 - -0.3 20,281 - +2.3 20,314 + BWI76_RS00105 0.12 +2.6 20,322 - BWI76_RS00105 0.14 +0.4 20,350 - BWI76_RS00105 0.19 -0.1 20,436 - BWI76_RS00105 0.34 +0.0 20,467 - BWI76_RS00105 0.39 +1.0 20,495 - BWI76_RS00105 0.44 +0.4 20,502 + BWI76_RS00105 0.46 +0.1 20,588 - BWI76_RS00105 0.61 -0.5 20,715 - BWI76_RS00105 0.83 -0.2 20,806 - -0.4 20,835 - -0.1 20,856 + +0.3 20,856 + +0.4 20,864 - +0.5 20,929 - -1.1 20,929 - +0.1 20,989 - -0.2 21,080 + BWI76_RS00110 0.16 +0.6 21,359 - BWI76_RS00110 0.37 +0.8 21,459 + BWI76_RS00110 0.45 +1.1 21,538 + BWI76_RS00110 0.51 +0.9 21,578 - BWI76_RS00110 0.54 +0.5 21,737 + BWI76_RS00110 0.65 +0.9 21,957 - BWI76_RS00110 0.82 +0.6 22,121 - +0.6 22,125 - +1.3 22,130 - +0.3 22,416 - +3.8 22,506 - BWI76_RS00115 0.21 +0.0 22,559 - BWI76_RS00115 0.29 +0.4 22,675 - -0.1 22,686 + BWI76_RS00115 0.48 -0.1 22,694 - BWI76_RS00115 0.50 +0.0 22,854 - BWI76_RS00115 0.74 +0.6 22,890 - BWI76_RS00115 0.79 +0.5 23,039 + +0.6 23,076 - +0.5 23,081 - +0.5 23,133 + +0.7 23,170 - BWI76_RS00120 0.13 +1.0
Or see this region's nucleotide sequence