Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23980 and BWI76_RS23985 are separated by 51 nucleotides BWI76_RS23985 and BWI76_RS23990 are separated by 377 nucleotides BWI76_RS23990 and BWI76_RS23995 are separated by 24 nucleotides
BWI76_RS23980: BWI76_RS23980 - phosphoglycerate kinase, at 5,005,248 to 5,006,411
_RS23980
BWI76_RS23985: BWI76_RS23985 - erythrose-4-phosphate dehydrogenase, at 5,006,463 to 5,007,482
_RS23985
BWI76_RS23990: BWI76_RS23990 - DNA-binding protein, at 5,007,860 to 5,008,285
_RS23990
BWI76_RS23995: BWI76_RS23995 - hypothetical protein, at 5,008,310 to 5,008,888
_RS23995
Position (kb)
5006
5007
5008 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5006.473 kb on - strand at 5006.518 kb on - strand at 5006.654 kb on + strand, within BWI76_RS23985 at 5006.738 kb on + strand, within BWI76_RS23985 at 5006.829 kb on - strand, within BWI76_RS23985 at 5006.963 kb on - strand, within BWI76_RS23985 at 5006.968 kb on - strand, within BWI76_RS23985 at 5007.007 kb on - strand, within BWI76_RS23985 at 5007.222 kb on + strand, within BWI76_RS23985 at 5007.241 kb on + strand, within BWI76_RS23985 at 5007.371 kb on + strand, within BWI76_RS23985 at 5007.458 kb on - strand at 5007.490 kb on + strand at 5007.490 kb on + strand at 5007.524 kb on + strand at 5007.532 kb on - strand at 5007.532 kb on - strand at 5007.539 kb on + strand at 5007.547 kb on - strand at 5007.625 kb on - strand at 5007.875 kb on + strand at 5007.897 kb on + strand at 5007.902 kb on - strand at 5007.958 kb on + strand, within BWI76_RS23990 at 5008.024 kb on - strand, within BWI76_RS23990 at 5008.155 kb on - strand, within BWI76_RS23990 at 5008.170 kb on + strand, within BWI76_RS23990 at 5008.178 kb on - strand, within BWI76_RS23990 at 5008.188 kb on + strand, within BWI76_RS23990 at 5008.196 kb on - strand, within BWI76_RS23990
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 5,006,473 - +0.2 5,006,518 - -0.3 5,006,654 + BWI76_RS23985 0.19 -1.6 5,006,738 + BWI76_RS23985 0.27 -0.4 5,006,829 - BWI76_RS23985 0.36 -0.8 5,006,963 - BWI76_RS23985 0.49 -2.3 5,006,968 - BWI76_RS23985 0.50 -0.9 5,007,007 - BWI76_RS23985 0.53 +0.2 5,007,222 + BWI76_RS23985 0.74 +0.1 5,007,241 + BWI76_RS23985 0.76 -0.2 5,007,371 + BWI76_RS23985 0.89 -2.2 5,007,458 - -0.6 5,007,490 + -2.5 5,007,490 + -1.1 5,007,524 + -0.8 5,007,532 - +0.3 5,007,532 - +0.8 5,007,539 + -1.6 5,007,547 - -0.8 5,007,625 - +0.5 5,007,875 + -1.6 5,007,897 + -2.9 5,007,902 - -0.9 5,007,958 + BWI76_RS23990 0.23 +0.1 5,008,024 - BWI76_RS23990 0.38 -0.7 5,008,155 - BWI76_RS23990 0.69 -1.0 5,008,170 + BWI76_RS23990 0.73 +0.0 5,008,178 - BWI76_RS23990 0.75 +0.2 5,008,188 + BWI76_RS23990 0.77 +0.3 5,008,196 - BWI76_RS23990 0.79 -0.1
Or see this region's nucleotide sequence