Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18425 and BWI76_RS18430 are separated by 42 nucleotides BWI76_RS18430 and BWI76_RS18435 are separated by 624 nucleotides BWI76_RS18435 and BWI76_RS18440 are separated by 483 nucleotides BWI76_RS18440 and BWI76_RS18445 overlap by 4 nucleotides
BWI76_RS18425: BWI76_RS18425 - phosphohydrolase, at 3,823,256 to 3,823,951
_RS18425
BWI76_RS18430: BWI76_RS18430 - hypothetical protein, at 3,823,994 to 3,824,275
_RS18430
BWI76_RS18435: BWI76_RS18435 - porin, at 3,824,900 to 3,825,970
_RS18435
BWI76_RS18440: BWI76_RS18440 - GtrA-like protein, at 3,826,454 to 3,826,843
_RS18440
BWI76_RS18445: BWI76_RS18445 - glycosyltransferase, at 3,826,840 to 3,827,832
_RS18445
Position (kb)
3824
3825
3826 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3823.972 kb on - strand at 3823.979 kb on - strand at 3823.994 kb on - strand at 3824.162 kb on - strand, within BWI76_RS18430 at 3824.173 kb on + strand, within BWI76_RS18430 at 3824.181 kb on - strand, within BWI76_RS18430 at 3824.196 kb on - strand, within BWI76_RS18430 at 3824.285 kb on - strand at 3824.316 kb on - strand at 3824.410 kb on - strand at 3824.511 kb on + strand at 3824.511 kb on + strand at 3824.511 kb on + strand at 3824.515 kb on + strand at 3824.515 kb on + strand at 3824.519 kb on - strand at 3824.523 kb on - strand at 3824.523 kb on - strand at 3824.523 kb on - strand at 3824.523 kb on - strand at 3824.534 kb on - strand at 3824.535 kb on + strand at 3824.537 kb on + strand at 3824.537 kb on + strand at 3824.543 kb on + strand at 3824.543 kb on + strand at 3824.543 kb on + strand at 3824.543 kb on + strand at 3824.544 kb on - strand at 3824.551 kb on - strand at 3824.551 kb on - strand at 3824.551 kb on - strand at 3824.551 kb on - strand at 3824.567 kb on + strand at 3824.587 kb on - strand at 3824.687 kb on - strand at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.025 kb on + strand, within BWI76_RS18435 at 3825.033 kb on - strand, within BWI76_RS18435 at 3825.033 kb on - strand at 3825.033 kb on - strand, within BWI76_RS18435 at 3825.033 kb on - strand, within BWI76_RS18435 at 3825.033 kb on - strand, within BWI76_RS18435 at 3825.033 kb on - strand, within BWI76_RS18435 at 3825.049 kb on + strand, within BWI76_RS18435 at 3825.060 kb on - strand, within BWI76_RS18435 at 3825.106 kb on - strand, within BWI76_RS18435 at 3825.123 kb on + strand, within BWI76_RS18435 at 3825.123 kb on + strand, within BWI76_RS18435 at 3825.123 kb on + strand, within BWI76_RS18435 at 3825.123 kb on + strand, within BWI76_RS18435 at 3825.123 kb on + strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.131 kb on - strand, within BWI76_RS18435 at 3825.133 kb on + strand, within BWI76_RS18435 at 3825.168 kb on - strand, within BWI76_RS18435 at 3825.170 kb on + strand, within BWI76_RS18435 at 3825.175 kb on + strand, within BWI76_RS18435 at 3825.175 kb on + strand, within BWI76_RS18435 at 3825.175 kb on + strand, within BWI76_RS18435 at 3825.175 kb on + strand, within BWI76_RS18435 at 3825.178 kb on - strand, within BWI76_RS18435 at 3825.178 kb on - strand, within BWI76_RS18435 at 3825.180 kb on + strand, within BWI76_RS18435 at 3825.180 kb on + strand, within BWI76_RS18435 at 3825.183 kb on - strand, within BWI76_RS18435 at 3825.183 kb on - strand, within BWI76_RS18435 at 3825.188 kb on - strand, within BWI76_RS18435 at 3825.188 kb on - strand, within BWI76_RS18435 at 3825.188 kb on - strand, within BWI76_RS18435 at 3825.197 kb on - strand, within BWI76_RS18435 at 3825.272 kb on + strand, within BWI76_RS18435 at 3825.272 kb on + strand, within BWI76_RS18435 at 3825.280 kb on - strand, within BWI76_RS18435 at 3825.313 kb on + strand, within BWI76_RS18435 at 3825.328 kb on + strand, within BWI76_RS18435 at 3825.336 kb on - strand, within BWI76_RS18435 at 3825.336 kb on - strand, within BWI76_RS18435 at 3825.336 kb on - strand, within BWI76_RS18435 at 3825.343 kb on + strand, within BWI76_RS18435 at 3825.351 kb on - strand, within BWI76_RS18435 at 3825.364 kb on + strand, within BWI76_RS18435 at 3825.364 kb on + strand, within BWI76_RS18435 at 3825.397 kb on + strand, within BWI76_RS18435 at 3825.498 kb on + strand, within BWI76_RS18435 at 3825.511 kb on - strand, within BWI76_RS18435 at 3825.515 kb on + strand, within BWI76_RS18435 at 3825.518 kb on + strand, within BWI76_RS18435 at 3825.520 kb on + strand, within BWI76_RS18435 at 3825.523 kb on - strand, within BWI76_RS18435 at 3825.528 kb on - strand, within BWI76_RS18435 at 3825.534 kb on + strand, within BWI76_RS18435 at 3825.621 kb on + strand, within BWI76_RS18435 at 3825.625 kb on + strand, within BWI76_RS18435 at 3825.689 kb on - strand, within BWI76_RS18435 at 3825.703 kb on + strand, within BWI76_RS18435 at 3825.703 kb on + strand, within BWI76_RS18435 at 3825.754 kb on + strand, within BWI76_RS18435 at 3825.754 kb on + strand, within BWI76_RS18435 at 3825.755 kb on + strand, within BWI76_RS18435 at 3825.757 kb on - strand, within BWI76_RS18435 at 3825.762 kb on - strand, within BWI76_RS18435 at 3825.763 kb on - strand, within BWI76_RS18435 at 3825.773 kb on + strand, within BWI76_RS18435 at 3825.774 kb on + strand, within BWI76_RS18435 at 3825.820 kb on - strand, within BWI76_RS18435 at 3825.827 kb on + strand, within BWI76_RS18435 at 3825.911 kb on + strand at 3825.919 kb on - strand at 3825.926 kb on + strand at 3825.940 kb on + strand at 3825.957 kb on - strand at 3825.962 kb on - strand at 3825.965 kb on - strand at 3825.984 kb on + strand at 3825.985 kb on + strand at 3826.022 kb on + strand at 3826.022 kb on + strand at 3826.022 kb on + strand at 3826.022 kb on + strand at 3826.022 kb on + strand at 3826.030 kb on - strand at 3826.030 kb on - strand at 3826.030 kb on - strand at 3826.030 kb on - strand at 3826.031 kb on - strand at 3826.044 kb on + strand at 3826.044 kb on - strand at 3826.044 kb on - strand at 3826.044 kb on - strand at 3826.044 kb on - strand at 3826.047 kb on + strand at 3826.047 kb on - strand at 3826.050 kb on + strand at 3826.058 kb on - strand at 3826.073 kb on - strand at 3826.073 kb on - strand at 3826.073 kb on - strand at 3826.073 kb on - strand at 3826.079 kb on - strand at 3826.114 kb on - strand at 3826.157 kb on - strand at 3826.177 kb on + strand at 3826.177 kb on + strand at 3826.177 kb on + strand at 3826.177 kb on + strand at 3826.177 kb on + strand at 3826.177 kb on + strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.185 kb on - strand at 3826.191 kb on + strand at 3826.199 kb on - strand at 3826.199 kb on - strand at 3826.202 kb on + strand at 3826.204 kb on + strand at 3826.205 kb on - strand at 3826.210 kb on - strand at 3826.210 kb on - strand at 3826.210 kb on - strand at 3826.210 kb on - strand at 3826.210 kb on - strand at 3826.226 kb on - strand at 3826.249 kb on - strand at 3826.255 kb on + strand at 3826.263 kb on - strand at 3826.289 kb on + strand at 3826.297 kb on - strand at 3826.297 kb on - strand at 3826.297 kb on - strand at 3826.297 kb on - strand at 3826.304 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.346 kb on - strand at 3826.348 kb on + strand at 3826.348 kb on + strand at 3826.348 kb on + strand at 3826.348 kb on + strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.356 kb on - strand at 3826.461 kb on + strand at 3826.468 kb on - strand at 3826.517 kb on - strand, within BWI76_RS18440 at 3826.519 kb on + strand, within BWI76_RS18440 at 3826.572 kb on - strand, within BWI76_RS18440 at 3826.574 kb on + strand, within BWI76_RS18440 at 3826.576 kb on + strand, within BWI76_RS18440 at 3826.576 kb on + strand, within BWI76_RS18440 at 3826.576 kb on + strand, within BWI76_RS18440 at 3826.582 kb on - strand, within BWI76_RS18440 at 3826.584 kb on - strand, within BWI76_RS18440 at 3826.584 kb on - strand, within BWI76_RS18440 at 3826.584 kb on - strand, within BWI76_RS18440 at 3826.598 kb on + strand, within BWI76_RS18440 at 3826.598 kb on + strand, within BWI76_RS18440 at 3826.598 kb on + strand, within BWI76_RS18440 at 3826.753 kb on + strand, within BWI76_RS18440 at 3826.761 kb on - strand, within BWI76_RS18440 at 3826.762 kb on + strand, within BWI76_RS18440 at 3826.770 kb on - strand, within BWI76_RS18440 at 3826.924 kb on + strand at 3826.928 kb on - strand at 3826.934 kb on + strand at 3826.942 kb on - strand, within BWI76_RS18445 at 3826.944 kb on + strand, within BWI76_RS18445
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 3,823,972 - -1.0 3,823,979 - -0.9 3,823,994 - +0.5 3,824,162 - BWI76_RS18430 0.60 -1.1 3,824,173 + BWI76_RS18430 0.63 +0.9 3,824,181 - BWI76_RS18430 0.66 -0.2 3,824,196 - BWI76_RS18430 0.72 -0.7 3,824,285 - -2.0 3,824,316 - +0.3 3,824,410 - +0.3 3,824,511 + +0.7 3,824,511 + +1.1 3,824,511 + +0.1 3,824,515 + -0.6 3,824,515 + +2.4 3,824,519 - +0.4 3,824,523 - +1.3 3,824,523 - +1.9 3,824,523 - +0.9 3,824,523 - +1.1 3,824,534 - +0.7 3,824,535 + -0.2 3,824,537 + -0.2 3,824,537 + +0.3 3,824,543 + +0.1 3,824,543 + +2.1 3,824,543 + -1.8 3,824,543 + -1.7 3,824,544 - +1.5 3,824,551 - -3.3 3,824,551 - -0.6 3,824,551 - +0.4 3,824,551 - -0.2 3,824,567 + -0.7 3,824,587 - -0.1 3,824,687 - -0.6 3,825,025 + BWI76_RS18435 0.12 -1.3 3,825,025 + BWI76_RS18435 0.12 +1.2 3,825,025 + BWI76_RS18435 0.12 +2.0 3,825,025 + BWI76_RS18435 0.12 -2.9 3,825,025 + BWI76_RS18435 0.12 -0.4 3,825,025 + BWI76_RS18435 0.12 +0.2 3,825,025 + BWI76_RS18435 0.12 -2.4 3,825,025 + BWI76_RS18435 0.12 -0.1 3,825,025 + BWI76_RS18435 0.12 +0.6 3,825,033 - BWI76_RS18435 0.12 -0.9 3,825,033 - +1.3 3,825,033 - BWI76_RS18435 0.12 -0.7 3,825,033 - BWI76_RS18435 0.12 -0.4 3,825,033 - BWI76_RS18435 0.12 -1.8 3,825,033 - BWI76_RS18435 0.12 +1.1 3,825,049 + BWI76_RS18435 0.14 +1.3 3,825,060 - BWI76_RS18435 0.15 +0.9 3,825,106 - BWI76_RS18435 0.19 -0.5 3,825,123 + BWI76_RS18435 0.21 -0.3 3,825,123 + BWI76_RS18435 0.21 -0.6 3,825,123 + BWI76_RS18435 0.21 -0.3 3,825,123 + BWI76_RS18435 0.21 +0.1 3,825,123 + BWI76_RS18435 0.21 +0.3 3,825,131 - BWI76_RS18435 0.22 -0.3 3,825,131 - BWI76_RS18435 0.22 +0.8 3,825,131 - BWI76_RS18435 0.22 -0.3 3,825,131 - BWI76_RS18435 0.22 -0.5 3,825,131 - BWI76_RS18435 0.22 -1.5 3,825,131 - BWI76_RS18435 0.22 +0.2 3,825,131 - BWI76_RS18435 0.22 +1.3 3,825,133 + BWI76_RS18435 0.22 -3.8 3,825,168 - BWI76_RS18435 0.25 -0.5 3,825,170 + BWI76_RS18435 0.25 -0.8 3,825,175 + BWI76_RS18435 0.26 +1.4 3,825,175 + BWI76_RS18435 0.26 -0.8 3,825,175 + BWI76_RS18435 0.26 +0.9 3,825,175 + BWI76_RS18435 0.26 +2.4 3,825,178 - BWI76_RS18435 0.26 -2.0 3,825,178 - BWI76_RS18435 0.26 -1.0 3,825,180 + BWI76_RS18435 0.26 -0.6 3,825,180 + BWI76_RS18435 0.26 -1.4 3,825,183 - BWI76_RS18435 0.26 -0.1 3,825,183 - BWI76_RS18435 0.26 +0.9 3,825,188 - BWI76_RS18435 0.27 -3.1 3,825,188 - BWI76_RS18435 0.27 -1.4 3,825,188 - BWI76_RS18435 0.27 -0.5 3,825,197 - BWI76_RS18435 0.28 -1.0 3,825,272 + BWI76_RS18435 0.35 -1.3 3,825,272 + BWI76_RS18435 0.35 -1.1 3,825,280 - BWI76_RS18435 0.35 -2.0 3,825,313 + BWI76_RS18435 0.39 +0.4 3,825,328 + BWI76_RS18435 0.40 -0.2 3,825,336 - BWI76_RS18435 0.41 -1.2 3,825,336 - BWI76_RS18435 0.41 +0.5 3,825,336 - BWI76_RS18435 0.41 -0.9 3,825,343 + BWI76_RS18435 0.41 -1.6 3,825,351 - BWI76_RS18435 0.42 +0.0 3,825,364 + BWI76_RS18435 0.43 +1.0 3,825,364 + BWI76_RS18435 0.43 -0.9 3,825,397 + BWI76_RS18435 0.46 +0.2 3,825,498 + BWI76_RS18435 0.56 +0.3 3,825,511 - BWI76_RS18435 0.57 -0.5 3,825,515 + BWI76_RS18435 0.57 -1.7 3,825,518 + BWI76_RS18435 0.58 -0.5 3,825,520 + BWI76_RS18435 0.58 -0.6 3,825,523 - BWI76_RS18435 0.58 -0.1 3,825,528 - BWI76_RS18435 0.59 -0.2 3,825,534 + BWI76_RS18435 0.59 +0.7 3,825,621 + BWI76_RS18435 0.67 -1.4 3,825,625 + BWI76_RS18435 0.68 -1.2 3,825,689 - BWI76_RS18435 0.74 -0.1 3,825,703 + BWI76_RS18435 0.75 +0.0 3,825,703 + BWI76_RS18435 0.75 -0.3 3,825,754 + BWI76_RS18435 0.80 -0.5 3,825,754 + BWI76_RS18435 0.80 -0.9 3,825,755 + BWI76_RS18435 0.80 -0.7 3,825,757 - BWI76_RS18435 0.80 -2.6 3,825,762 - BWI76_RS18435 0.80 -0.7 3,825,763 - BWI76_RS18435 0.81 +0.6 3,825,773 + BWI76_RS18435 0.82 +0.2 3,825,774 + BWI76_RS18435 0.82 -0.1 3,825,820 - BWI76_RS18435 0.86 +0.2 3,825,827 + BWI76_RS18435 0.87 -0.3 3,825,911 + +0.5 3,825,919 - +0.8 3,825,926 + +0.5 3,825,940 + +0.4 3,825,957 - +1.8 3,825,962 - -3.1 3,825,965 - +0.5 3,825,984 + -0.9 3,825,985 + -0.7 3,826,022 + +0.4 3,826,022 + -0.2 3,826,022 + -0.7 3,826,022 + -0.2 3,826,022 + +0.5 3,826,030 - -0.2 3,826,030 - -0.7 3,826,030 - -0.7 3,826,030 - -0.6 3,826,031 - -1.4 3,826,044 + +0.4 3,826,044 - -2.9 3,826,044 - -1.1 3,826,044 - -2.8 3,826,044 - +1.2 3,826,047 + +1.6 3,826,047 - -0.7 3,826,050 + -2.1 3,826,058 - -0.1 3,826,073 - +0.7 3,826,073 - -3.6 3,826,073 - -0.9 3,826,073 - -0.3 3,826,079 - +0.7 3,826,114 - -2.0 3,826,157 - -0.6 3,826,177 + +1.2 3,826,177 + -1.1 3,826,177 + -0.5 3,826,177 + -0.2 3,826,177 + -0.8 3,826,177 + +0.3 3,826,185 - +0.2 3,826,185 - -2.6 3,826,185 - -0.2 3,826,185 - -1.4 3,826,185 - -3.9 3,826,185 - -0.4 3,826,185 - +0.1 3,826,185 - -0.1 3,826,185 - +0.4 3,826,185 - -0.2 3,826,185 - +1.6 3,826,185 - +0.1 3,826,185 - +0.1 3,826,185 - -0.9 3,826,185 - -0.9 3,826,185 - -1.2 3,826,185 - -0.8 3,826,191 + -0.2 3,826,199 - +0.1 3,826,199 - -1.7 3,826,202 + -3.1 3,826,204 + +0.5 3,826,205 - -2.6 3,826,210 - -0.0 3,826,210 - -0.9 3,826,210 - +0.7 3,826,210 - +0.1 3,826,210 - +0.3 3,826,226 - -1.9 3,826,249 - -0.8 3,826,255 + +0.2 3,826,263 - -1.7 3,826,289 + +0.9 3,826,297 - +0.3 3,826,297 - -1.5 3,826,297 - +1.0 3,826,297 - -0.3 3,826,304 - -0.2 3,826,346 - +0.0 3,826,346 - +0.6 3,826,346 - +1.9 3,826,346 - -0.5 3,826,346 - +0.0 3,826,346 - +1.4 3,826,346 - +0.2 3,826,348 + -1.1 3,826,348 + -0.9 3,826,348 + +0.1 3,826,348 + -0.8 3,826,356 - -1.5 3,826,356 - +0.1 3,826,356 - -0.4 3,826,356 - +0.1 3,826,356 - -0.5 3,826,356 - +0.5 3,826,356 - -0.8 3,826,356 - +0.5 3,826,356 - -1.2 3,826,356 - -0.7 3,826,356 - +2.2 3,826,356 - +0.1 3,826,356 - -3.4 3,826,356 - -0.6 3,826,461 + +0.5 3,826,468 - +0.6 3,826,517 - BWI76_RS18440 0.16 +1.5 3,826,519 + BWI76_RS18440 0.17 -0.3 3,826,572 - BWI76_RS18440 0.30 -2.0 3,826,574 + BWI76_RS18440 0.31 -1.1 3,826,576 + BWI76_RS18440 0.31 -0.8 3,826,576 + BWI76_RS18440 0.31 -0.7 3,826,576 + BWI76_RS18440 0.31 -0.3 3,826,582 - BWI76_RS18440 0.33 +0.3 3,826,584 - BWI76_RS18440 0.33 +0.9 3,826,584 - BWI76_RS18440 0.33 +1.6 3,826,584 - BWI76_RS18440 0.33 -0.8 3,826,598 + BWI76_RS18440 0.37 -0.7 3,826,598 + BWI76_RS18440 0.37 +0.3 3,826,598 + BWI76_RS18440 0.37 -1.0 3,826,753 + BWI76_RS18440 0.77 +3.9 3,826,761 - BWI76_RS18440 0.79 +0.1 3,826,762 + BWI76_RS18440 0.79 +0.3 3,826,770 - BWI76_RS18440 0.81 -1.3 3,826,924 + -0.4 3,826,928 - -0.4 3,826,934 + -2.1 3,826,942 - BWI76_RS18445 0.10 +0.8 3,826,944 + BWI76_RS18445 0.10 -0.2
Or see this region's nucleotide sequence