Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17565 and BWI76_RS17570 are separated by 65 nucleotides BWI76_RS17570 and BWI76_RS17575 are separated by 12 nucleotides
BWI76_RS17565: BWI76_RS17565 - hypothetical protein, at 3,647,487 to 3,648,092
_RS17565
BWI76_RS17570: BWI76_RS17570 - iron-sulfur-binding protein, at 3,648,158 to 3,648,616
_RS17570
BWI76_RS17575: BWI76_RS17575 - hypothetical protein, at 3,648,629 to 3,651,841
_RS17575
Position (kb)
3648
3649
3650
3651
3652 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3647.834 kb on + strand, within BWI76_RS17565 at 3648.411 kb on - strand, within BWI76_RS17570 at 3648.498 kb on - strand, within BWI76_RS17570 at 3648.905 kb on + strand at 3648.928 kb on - strand at 3649.014 kb on + strand, within BWI76_RS17575 at 3649.090 kb on + strand, within BWI76_RS17575 at 3649.186 kb on + strand, within BWI76_RS17575 at 3649.304 kb on - strand, within BWI76_RS17575 at 3649.522 kb on - strand, within BWI76_RS17575 at 3649.561 kb on - strand, within BWI76_RS17575 at 3649.859 kb on - strand, within BWI76_RS17575 at 3649.908 kb on + strand, within BWI76_RS17575 at 3649.973 kb on + strand, within BWI76_RS17575 at 3650.116 kb on + strand, within BWI76_RS17575 at 3650.174 kb on - strand, within BWI76_RS17575 at 3650.490 kb on - strand, within BWI76_RS17575 at 3650.844 kb on - strand, within BWI76_RS17575 at 3650.874 kb on - strand, within BWI76_RS17575 at 3650.874 kb on - strand, within BWI76_RS17575 at 3650.963 kb on + strand, within BWI76_RS17575 at 3651.152 kb on + strand, within BWI76_RS17575 at 3651.283 kb on + strand, within BWI76_RS17575 at 3651.368 kb on - strand, within BWI76_RS17575 at 3651.617 kb on - strand at 3651.715 kb on - strand at 3651.782 kb on - strand at 3651.812 kb on - strand at 3652.543 kb on + strand at 3652.546 kb on - strand at 3652.606 kb on - strand at 3652.606 kb on - strand at 3652.609 kb on + strand at 3652.615 kb on - strand at 3652.681 kb on + strand at 3652.719 kb on + strand at 3652.719 kb on + strand at 3652.719 kb on + strand at 3652.719 kb on + strand at 3652.719 kb on + strand at 3652.719 kb on + strand at 3652.727 kb on - strand at 3652.727 kb on - strand at 3652.727 kb on - strand at 3652.727 kb on - strand at 3652.727 kb on - strand at 3652.798 kb on - strand at 3652.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 3,647,834 + BWI76_RS17565 0.57 -2.4 3,648,411 - BWI76_RS17570 0.55 +0.4 3,648,498 - BWI76_RS17570 0.74 -0.5 3,648,905 + +0.3 3,648,928 - -0.5 3,649,014 + BWI76_RS17575 0.12 +2.9 3,649,090 + BWI76_RS17575 0.14 -0.2 3,649,186 + BWI76_RS17575 0.17 +0.5 3,649,304 - BWI76_RS17575 0.21 -0.5 3,649,522 - BWI76_RS17575 0.28 -0.0 3,649,561 - BWI76_RS17575 0.29 -1.2 3,649,859 - BWI76_RS17575 0.38 +0.0 3,649,908 + BWI76_RS17575 0.40 +0.7 3,649,973 + BWI76_RS17575 0.42 -0.7 3,650,116 + BWI76_RS17575 0.46 -0.7 3,650,174 - BWI76_RS17575 0.48 -0.5 3,650,490 - BWI76_RS17575 0.58 -1.7 3,650,844 - BWI76_RS17575 0.69 -2.5 3,650,874 - BWI76_RS17575 0.70 +1.0 3,650,874 - BWI76_RS17575 0.70 -0.8 3,650,963 + BWI76_RS17575 0.73 -0.8 3,651,152 + BWI76_RS17575 0.79 -0.6 3,651,283 + BWI76_RS17575 0.83 -4.3 3,651,368 - BWI76_RS17575 0.85 -0.2 3,651,617 - -0.9 3,651,715 - -1.1 3,651,782 - -1.2 3,651,812 - -2.6 3,652,543 + +1.3 3,652,546 - +2.1 3,652,606 - -0.0 3,652,606 - -0.1 3,652,609 + +2.2 3,652,615 - -1.7 3,652,681 + -2.7 3,652,719 + -1.3 3,652,719 + -2.2 3,652,719 + -0.4 3,652,719 + -2.1 3,652,719 + -0.3 3,652,719 + +1.1 3,652,727 - -0.4 3,652,727 - -0.8 3,652,727 - +0.2 3,652,727 - -0.5 3,652,727 - -1.9 3,652,798 - +0.4 3,652,801 - -0.6
Or see this region's nucleotide sequence