Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17415 and BWI76_RS17420 are separated by 39 nucleotides BWI76_RS17420 and BWI76_RS17425 are separated by 3 nucleotides BWI76_RS17425 and BWI76_RS17430 overlap by 4 nucleotides BWI76_RS17430 and BWI76_RS17435 overlap by 1 nucleotides
BWI76_RS17415: BWI76_RS17415 - 3-deoxy-8-phosphooctulonate synthase, at 3,612,582 to 3,613,436
_RS17415
BWI76_RS17420: BWI76_RS17420 - hypothetical protein, at 3,613,476 to 3,614,288
_RS17420
BWI76_RS17425: BWI76_RS17425 - hypothetical protein, at 3,614,292 to 3,614,684
_RS17425
BWI76_RS17430: BWI76_RS17430 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 3,614,681 to 3,615,520
_RS17430
BWI76_RS17435: BWI76_RS17435 - peptide chain release factor 1, at 3,615,520 to 3,616,602
_RS17435
Position (kb) 3614
3615 Strain fitness (log2 ratio) -1
0
1
2 at 3613.611 kb on - strand, within BWI76_RS17420 at 3613.979 kb on - strand, within BWI76_RS17420 at 3614.032 kb on - strand, within BWI76_RS17420 at 3614.462 kb on + strand, within BWI76_RS17425 at 3614.470 kb on - strand, within BWI76_RS17425 at 3614.653 kb on + strand at 3614.805 kb on - strand, within BWI76_RS17430
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 3,613,611 - BWI76_RS17420 0.17 +0.3 3,613,979 - BWI76_RS17420 0.62 +0.0 3,614,032 - BWI76_RS17420 0.68 +1.9 3,614,462 + BWI76_RS17425 0.43 +1.2 3,614,470 - BWI76_RS17425 0.45 +0.3 3,614,653 + -1.7 3,614,805 - BWI76_RS17430 0.15 -0.5
Or see this region's nucleotide sequence