Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14470

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14460 and BWI76_RS14465 are separated by 329 nucleotidesBWI76_RS14465 and BWI76_RS14470 are separated by 67 nucleotidesBWI76_RS14470 and BWI76_RS14475 are separated by 62 nucleotides BWI76_RS14460: BWI76_RS14460 - MurR/RpiR family transcriptional regulator, at 3,017,412 to 3,018,269 _RS14460 BWI76_RS14465: BWI76_RS14465 - transcriptional regulator, at 3,018,599 to 3,019,042 _RS14465 BWI76_RS14470: BWI76_RS14470 - L-serine ammonia-lyase, at 3,019,110 to 3,020,477 _RS14470 BWI76_RS14475: BWI76_RS14475 - serine/threonine transporter, at 3,020,540 to 3,021,823 _RS14475 Position (kb) 3019 3020 3021Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3018.128 kb on + strand, within BWI76_RS14460at 3018.128 kb on + strand, within BWI76_RS14460at 3018.223 kb on + strandat 3018.286 kb on - strandat 3018.324 kb on - strandat 3018.375 kb on - strandat 3018.387 kb on - strandat 3018.395 kb on - strandat 3018.422 kb on - strandat 3018.614 kb on + strandat 3018.617 kb on - strandat 3018.617 kb on - strandat 3018.643 kb on + strandat 3018.671 kb on + strand, within BWI76_RS14465at 3018.671 kb on + strand, within BWI76_RS14465at 3018.726 kb on + strand, within BWI76_RS14465at 3018.759 kb on + strand, within BWI76_RS14465at 3018.967 kb on + strand, within BWI76_RS14465at 3018.968 kb on + strand, within BWI76_RS14465at 3018.970 kb on + strand, within BWI76_RS14465at 3018.983 kb on + strand, within BWI76_RS14465at 3018.983 kb on + strand, within BWI76_RS14465at 3018.983 kb on + strand, within BWI76_RS14465at 3018.991 kb on - strand, within BWI76_RS14465at 3019.003 kb on - strandat 3019.004 kb on - strandat 3019.050 kb on + strandat 3019.218 kb on - strandat 3019.443 kb on + strand, within BWI76_RS14470at 3019.500 kb on - strand, within BWI76_RS14470at 3019.684 kb on + strandat 3019.759 kb on - strand, within BWI76_RS14470at 3019.924 kb on + strand, within BWI76_RS14470at 3019.947 kb on - strand, within BWI76_RS14470at 3019.950 kb on + strand, within BWI76_RS14470at 3020.024 kb on + strand, within BWI76_RS14470at 3020.206 kb on - strand, within BWI76_RS14470at 3020.270 kb on + strand, within BWI76_RS14470at 3020.323 kb on + strand, within BWI76_RS14470at 3020.334 kb on - strandat 3020.361 kb on - strandat 3020.414 kb on + strandat 3020.621 kb on + strandat 3020.834 kb on - strand, within BWI76_RS14475at 3020.904 kb on + strand, within BWI76_RS14475at 3020.946 kb on - strand, within BWI76_RS14475at 3020.975 kb on + strand, within BWI76_RS14475at 3021.074 kb on + strand, within BWI76_RS14475at 3021.074 kb on + strand, within BWI76_RS14475at 3021.082 kb on - strand, within BWI76_RS14475at 3021.249 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.259 kb on - strandat 3021.259 kb on - strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.380 kb on - strand, within BWI76_RS14475

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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3,018,128 + BWI76_RS14460 0.83 +0.0
3,018,128 + BWI76_RS14460 0.83 -1.2
3,018,223 + +2.2
3,018,286 - -0.2
3,018,324 - -0.0
3,018,375 - -0.0
3,018,387 - -1.2
3,018,395 - +0.2
3,018,422 - +0.2
3,018,614 + +0.2
3,018,617 - -2.2
3,018,617 - -1.2
3,018,643 + -0.8
3,018,671 + BWI76_RS14465 0.16 -1.5
3,018,671 + BWI76_RS14465 0.16 +2.5
3,018,726 + BWI76_RS14465 0.29 +1.8
3,018,759 + BWI76_RS14465 0.36 +0.8
3,018,967 + BWI76_RS14465 0.83 +0.3
3,018,968 + BWI76_RS14465 0.83 +1.3
3,018,970 + BWI76_RS14465 0.84 -1.8
3,018,983 + BWI76_RS14465 0.86 +0.3
3,018,983 + BWI76_RS14465 0.86 +0.9
3,018,983 + BWI76_RS14465 0.86 +0.9
3,018,991 - BWI76_RS14465 0.88 -0.4
3,019,003 - +1.7
3,019,004 - +0.7
3,019,050 + -0.7
3,019,218 - -1.3
3,019,443 + BWI76_RS14470 0.24 +3.4
3,019,500 - BWI76_RS14470 0.29 -3.0
3,019,684 + -0.2
3,019,759 - BWI76_RS14470 0.47 +0.4
3,019,924 + BWI76_RS14470 0.60 +1.0
3,019,947 - BWI76_RS14470 0.61 -0.8
3,019,950 + BWI76_RS14470 0.61 +1.2
3,020,024 + BWI76_RS14470 0.67 +1.3
3,020,206 - BWI76_RS14470 0.80 -0.9
3,020,270 + BWI76_RS14470 0.85 -2.3
3,020,323 + BWI76_RS14470 0.89 +1.6
3,020,334 - +0.2
3,020,361 - -0.5
3,020,414 + -2.4
3,020,621 + +0.5
3,020,834 - BWI76_RS14475 0.23 +0.8
3,020,904 + BWI76_RS14475 0.28 +0.5
3,020,946 - BWI76_RS14475 0.32 -1.0
3,020,975 + BWI76_RS14475 0.34 -1.2
3,021,074 + BWI76_RS14475 0.42 +1.2
3,021,074 + BWI76_RS14475 0.42 -0.3
3,021,082 - BWI76_RS14475 0.42 +0.9
3,021,249 - BWI76_RS14475 0.55 -0.2
3,021,257 - BWI76_RS14475 0.56 -0.4
3,021,257 - BWI76_RS14475 0.56 -1.4
3,021,259 - +0.6
3,021,259 - BWI76_RS14475 0.56 -1.6
3,021,333 + BWI76_RS14475 0.62 -0.2
3,021,333 + BWI76_RS14475 0.62 +0.0
3,021,341 - BWI76_RS14475 0.62 -0.4
3,021,341 - BWI76_RS14475 0.62 +1.1
3,021,341 - BWI76_RS14475 0.62 -1.5
3,021,380 - BWI76_RS14475 0.65 +1.2

Or see this region's nucleotide sequence