Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14400

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14390 and BWI76_RS14395 are separated by 517 nucleotidesBWI76_RS14395 and BWI76_RS14400 are separated by 110 nucleotidesBWI76_RS14400 and BWI76_RS14405 are separated by 120 nucleotidesBWI76_RS14405 and BWI76_RS14410 are separated by 91 nucleotidesBWI76_RS14410 and BWI76_RS14415 are separated by 118 nucleotides BWI76_RS14390: BWI76_RS14390 - hypothetical protein, at 3,006,987 to 3,007,862 _RS14390 BWI76_RS14395: BWI76_RS14395 - hypothetical protein, at 3,008,380 to 3,008,517 _RS14395 BWI76_RS14400: BWI76_RS14400 - ISNCY family transposase, at 3,008,628 to 3,009,198 _RS14400 BWI76_RS14405: BWI76_RS14405 - steroid Delta-isomerase, at 3,009,319 to 3,009,672 _RS14405 BWI76_RS14410: BWI76_RS14410 - hypothetical protein, at 3,009,764 to 3,009,967 _RS14410 BWI76_RS14415: BWI76_RS14415 - transcriptional regulator, TetR family protein, at 3,010,086 to 3,010,682 _RS14415 Position (kb) 3008 3009 3010Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3007.860 kb on + strandat 3008.043 kb on + strandat 3008.043 kb on + strandat 3008.045 kb on + strandat 3008.045 kb on + strandat 3008.077 kb on - strandat 3008.160 kb on - strandat 3008.247 kb on + strandat 3008.260 kb on + strandat 3008.260 kb on + strandat 3008.605 kb on - strandat 3008.909 kb on + strand, within BWI76_RS14400at 3008.909 kb on + strand, within BWI76_RS14400at 3008.959 kb on - strand, within BWI76_RS14400at 3009.019 kb on - strand, within BWI76_RS14400at 3009.084 kb on + strand, within BWI76_RS14400at 3009.096 kb on + strand, within BWI76_RS14400at 3009.096 kb on + strand, within BWI76_RS14400at 3009.096 kb on + strand, within BWI76_RS14400at 3009.096 kb on + strand, within BWI76_RS14400at 3009.096 kb on + strand, within BWI76_RS14400at 3009.130 kb on - strand, within BWI76_RS14400at 3009.130 kb on - strand, within BWI76_RS14400at 3009.178 kb on + strandat 3009.178 kb on + strandat 3009.178 kb on + strandat 3009.251 kb on + strandat 3009.264 kb on - strandat 3009.766 kb on - strandat 3009.812 kb on - strand, within BWI76_RS14410at 3009.822 kb on - strand, within BWI76_RS14410at 3009.822 kb on - strand, within BWI76_RS14410at 3009.822 kb on - strand, within BWI76_RS14410at 3009.848 kb on + strand, within BWI76_RS14410at 3009.856 kb on - strandat 3009.962 kb on + strandat 3009.970 kb on - strandat 3009.970 kb on - strandat 3009.986 kb on + strandat 3009.989 kb on - strandat 3009.994 kb on - strandat 3009.994 kb on - strandat 3009.994 kb on - strandat 3009.994 kb on - strandat 3010.128 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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3,007,860 + +1.1
3,008,043 + -1.7
3,008,043 + -0.2
3,008,045 + -0.7
3,008,045 + -2.3
3,008,077 - -0.4
3,008,160 - +2.5
3,008,247 + -1.4
3,008,260 + +1.2
3,008,260 + +2.2
3,008,605 - +0.2
3,008,909 + BWI76_RS14400 0.49 -1.4
3,008,909 + BWI76_RS14400 0.49 -1.7
3,008,959 - BWI76_RS14400 0.58 -2.2
3,009,019 - BWI76_RS14400 0.68 +0.3
3,009,084 + BWI76_RS14400 0.80 +0.3
3,009,096 + BWI76_RS14400 0.82 -0.7
3,009,096 + BWI76_RS14400 0.82 +1.7
3,009,096 + BWI76_RS14400 0.82 -1.0
3,009,096 + BWI76_RS14400 0.82 +2.2
3,009,096 + BWI76_RS14400 0.82 -0.8
3,009,130 - BWI76_RS14400 0.88 -1.0
3,009,130 - BWI76_RS14400 0.88 -1.3
3,009,178 + +1.3
3,009,178 + -1.8
3,009,178 + -2.7
3,009,251 + +3.7
3,009,264 - +2.1
3,009,766 - +1.2
3,009,812 - BWI76_RS14410 0.24 +1.2
3,009,822 - BWI76_RS14410 0.28 +2.2
3,009,822 - BWI76_RS14410 0.28 -1.4
3,009,822 - BWI76_RS14410 0.28 +0.8
3,009,848 + BWI76_RS14410 0.41 +1.6
3,009,856 - -0.2
3,009,962 + +1.8
3,009,970 - +0.8
3,009,970 - -1.4
3,009,986 + +0.6
3,009,989 - +2.5
3,009,994 - +0.8
3,009,994 - +0.2
3,009,994 - -0.4
3,009,994 - -0.2
3,010,128 + +2.4

Or see this region's nucleotide sequence