Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14285

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotidesBWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotidesBWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotides BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789 _RS14275 BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121 _RS14280 BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722 _RS14285 BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368 _RS14290 Position (kb) 2987 2988Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2986.439 kb on - strand, within BWI76_RS14275at 2986.457 kb on - strand, within BWI76_RS14275at 2986.542 kb on - strand, within BWI76_RS14275at 2986.613 kb on - strand, within BWI76_RS14275at 2986.614 kb on + strand, within BWI76_RS14275at 2986.839 kb on - strandat 2986.875 kb on + strandat 2986.942 kb on + strand, within BWI76_RS14280at 2986.993 kb on + strand, within BWI76_RS14280at 2987.043 kb on - strand, within BWI76_RS14280at 2987.173 kb on + strandat 2987.185 kb on - strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.250 kb on - strandat 2987.275 kb on + strandat 2987.276 kb on + strandat 2987.293 kb on - strandat 2987.401 kb on + strandat 2987.446 kb on + strandat 2987.481 kb on - strand, within BWI76_RS14285at 2987.491 kb on - strand, within BWI76_RS14285at 2987.514 kb on - strand, within BWI76_RS14285at 2987.520 kb on + strand, within BWI76_RS14285at 2987.524 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.878 kb on + strand, within BWI76_RS14290at 2987.886 kb on - strand, within BWI76_RS14290at 2987.898 kb on + strand, within BWI76_RS14290at 2987.921 kb on + strand, within BWI76_RS14290at 2987.955 kb on + strand, within BWI76_RS14290at 2988.029 kb on + strand, within BWI76_RS14290at 2988.079 kb on + strand, within BWI76_RS14290at 2988.231 kb on - strand, within BWI76_RS14290at 2988.344 kb on + strandat 2988.463 kb on - strandat 2988.472 kb on - strandat 2988.516 kb on + strandat 2988.531 kb on - strandat 2988.586 kb on - strandat 2988.596 kb on - strandat 2988.621 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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2,986,439 - BWI76_RS14275 0.71 -0.9
2,986,457 - BWI76_RS14275 0.72 -0.0
2,986,542 - BWI76_RS14275 0.79 -1.2
2,986,613 - BWI76_RS14275 0.85 -0.4
2,986,614 + BWI76_RS14275 0.85 +0.5
2,986,839 - +2.0
2,986,875 + +0.1
2,986,942 + BWI76_RS14280 0.30 +0.3
2,986,993 + BWI76_RS14280 0.50 +0.2
2,987,043 - BWI76_RS14280 0.69 -0.4
2,987,173 + +0.9
2,987,185 - -1.9
2,987,194 + +0.1
2,987,194 + -1.0
2,987,194 + -0.0
2,987,194 + -0.8
2,987,194 + -0.2
2,987,194 + -1.0
2,987,194 + +1.0
2,987,194 + -0.2
2,987,194 + -1.9
2,987,194 + +0.0
2,987,194 + +0.6
2,987,194 + -0.9
2,987,194 + +0.8
2,987,194 + +0.6
2,987,194 + +1.1
2,987,202 - +0.9
2,987,202 - +0.7
2,987,202 - +0.6
2,987,202 - -1.4
2,987,202 - +2.1
2,987,202 - +1.2
2,987,202 - -0.5
2,987,202 - +0.6
2,987,202 - -2.9
2,987,202 - +1.2
2,987,202 - -1.4
2,987,250 - +0.9
2,987,275 + -1.3
2,987,276 + -0.2
2,987,293 - -0.5
2,987,401 + +2.4
2,987,446 + +0.1
2,987,481 - BWI76_RS14285 0.17 -1.3
2,987,491 - BWI76_RS14285 0.20 -2.3
2,987,514 - BWI76_RS14285 0.28 -1.1
2,987,520 + BWI76_RS14285 0.30 -0.6
2,987,524 + BWI76_RS14285 0.32 -0.5
2,987,534 + BWI76_RS14285 0.35 -0.2
2,987,534 + BWI76_RS14285 0.35 -2.4
2,987,878 + BWI76_RS14290 0.19 -0.8
2,987,886 - BWI76_RS14290 0.20 -0.4
2,987,898 + BWI76_RS14290 0.22 +3.5
2,987,921 + BWI76_RS14290 0.26 -2.0
2,987,955 + BWI76_RS14290 0.31 +1.1
2,988,029 + BWI76_RS14290 0.44 +2.2
2,988,079 + BWI76_RS14290 0.52 -1.6
2,988,231 - BWI76_RS14290 0.77 +0.1
2,988,344 + -3.8
2,988,463 - -0.3
2,988,472 - +0.3
2,988,516 + +0.6
2,988,531 - -0.5
2,988,586 - -0.4
2,988,596 - -0.2
2,988,621 - -0.6

Or see this region's nucleotide sequence