Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13485 and BWI76_RS13490 are separated by 44 nucleotides BWI76_RS13490 and BWI76_RS13495 are separated by 291 nucleotides BWI76_RS13495 and BWI76_RS13500 are separated by 72 nucleotides
BWI76_RS13485: BWI76_RS13485 - NADP-dependent oxidoreductase, at 2,810,923 to 2,811,960
_RS13485
BWI76_RS13490: BWI76_RS13490 - pyrimidine-specific ribonucleoside hydrolase RihA, at 2,812,005 to 2,812,940
_RS13490
BWI76_RS13495: BWI76_RS13495 - hypothetical protein, at 2,813,232 to 2,813,531
_RS13495
BWI76_RS13500: BWI76_RS13500 - TonB-dependent receptor, at 2,813,604 to 2,815,715
_RS13500
Position (kb)
2812
2813 Strain fitness (log2 ratio)
-2
-1
0
1 at 2811.278 kb on + strand, within BWI76_RS13485 at 2811.343 kb on - strand, within BWI76_RS13485 at 2811.413 kb on - strand, within BWI76_RS13485 at 2811.521 kb on - strand, within BWI76_RS13485 at 2812.117 kb on + strand, within BWI76_RS13490 at 2812.202 kb on + strand, within BWI76_RS13490 at 2812.303 kb on + strand, within BWI76_RS13490 at 2812.370 kb on + strand, within BWI76_RS13490 at 2812.646 kb on - strand at 2812.768 kb on + strand, within BWI76_RS13490 at 2812.864 kb on + strand at 2813.007 kb on + strand at 2813.007 kb on + strand at 2813.007 kb on + strand at 2813.020 kb on - strand at 2813.025 kb on - strand at 2813.247 kb on + strand at 2813.259 kb on + strand at 2813.259 kb on + strand at 2813.267 kb on - strand, within BWI76_RS13495 at 2813.267 kb on - strand, within BWI76_RS13495 at 2813.455 kb on + strand, within BWI76_RS13495 at 2813.548 kb on + strand at 2813.800 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 2,811,278 + BWI76_RS13485 0.34 +0.0 2,811,343 - BWI76_RS13485 0.40 -0.2 2,811,413 - BWI76_RS13485 0.47 +0.6 2,811,521 - BWI76_RS13485 0.58 -0.1 2,812,117 + BWI76_RS13490 0.12 +0.8 2,812,202 + BWI76_RS13490 0.21 -1.9 2,812,303 + BWI76_RS13490 0.32 -2.7 2,812,370 + BWI76_RS13490 0.39 -1.9 2,812,646 - -0.5 2,812,768 + BWI76_RS13490 0.82 -0.7 2,812,864 + -1.1 2,813,007 + +0.5 2,813,007 + +0.3 2,813,007 + +0.6 2,813,020 - -0.7 2,813,025 - -0.7 2,813,247 + +1.3 2,813,259 + -1.2 2,813,259 + -0.0 2,813,267 - BWI76_RS13495 0.12 +0.5 2,813,267 - BWI76_RS13495 0.12 +0.1 2,813,455 + BWI76_RS13495 0.74 -0.0 2,813,548 + +1.6 2,813,800 + -0.2
Or see this region's nucleotide sequence