Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13145 and BWI76_RS13150 are separated by 9 nucleotides BWI76_RS13150 and BWI76_RS13155 are separated by 11 nucleotides BWI76_RS13155 and BWI76_RS13160 are separated by 35 nucleotides BWI76_RS13160 and BWI76_RS13165 are separated by 204 nucleotides
BWI76_RS13145: BWI76_RS13145 - phenylacetic acid degradation operon negative regulatory protein PaaX, at 2,741,574 to 2,742,509
_RS13145
BWI76_RS13150: BWI76_RS13150 - carnitine operon protein CaiE, at 2,742,519 to 2,743,115
_RS13150
BWI76_RS13155: BWI76_RS13155 - hypothetical protein, at 2,743,127 to 2,743,534
_RS13155
BWI76_RS13160: BWI76_RS13160 - azoreductase, at 2,743,570 to 2,744,175
_RS13160
BWI76_RS13165: BWI76_RS13165 - ATP-dependent RNA helicase HrpA, at 2,744,380 to 2,748,282
_RS13165
Position (kb) 2743
2744 Strain fitness (log2 ratio) -1
0
1 at 2742.728 kb on + strand, within BWI76_RS13150 at 2743.184 kb on - strand, within BWI76_RS13155 at 2743.203 kb on - strand, within BWI76_RS13155 at 2743.294 kb on + strand, within BWI76_RS13155 at 2743.302 kb on - strand, within BWI76_RS13155 at 2743.707 kb on + strand, within BWI76_RS13160 at 2744.165 kb on + strand at 2744.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 2,742,728 + BWI76_RS13150 0.35 -0.4 2,743,184 - BWI76_RS13155 0.14 -0.7 2,743,203 - BWI76_RS13155 0.19 -1.5 2,743,294 + BWI76_RS13155 0.41 -0.4 2,743,302 - BWI76_RS13155 0.43 -1.2 2,743,707 + BWI76_RS13160 0.23 +0.4 2,744,165 + +0.4 2,744,220 - -0.9
Or see this region's nucleotide sequence