Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11330 and BWI76_RS11335 are separated by 156 nucleotides BWI76_RS11335 and BWI76_RS11340 are separated by 5 nucleotides BWI76_RS11340 and BWI76_RS11345 are separated by 100 nucleotides BWI76_RS11345 and BWI76_RS11350 are separated by 71 nucleotides
BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067
_RS11330
BWI76_RS11335: BWI76_RS11335 - tautomerase, at 2,389,224 to 2,389,610
_RS11335
BWI76_RS11340: BWI76_RS11340 - carboxymuconolactone decarboxylase, at 2,389,616 to 2,389,918
_RS11340
BWI76_RS11345: BWI76_RS11345 - LysR family transcriptional regulator, at 2,390,019 to 2,390,903
_RS11345
BWI76_RS11350: BWI76_RS11350 - DeoR family transcriptional regulator, at 2,390,975 to 2,392,759
_RS11350
Position (kb)
2390
2391 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2389.183 kb on - strand at 2389.230 kb on - strand at 2389.232 kb on + strand at 2389.346 kb on + strand, within BWI76_RS11335 at 2389.581 kb on - strand at 2389.613 kb on + strand at 2389.748 kb on - strand at 2390.229 kb on + strand, within BWI76_RS11345 at 2390.595 kb on + strand, within BWI76_RS11345 at 2390.605 kb on + strand, within BWI76_RS11345 at 2390.859 kb on + strand at 2391.025 kb on - strand at 2391.135 kb on + strand at 2391.175 kb on - strand, within BWI76_RS11350 at 2391.383 kb on - strand, within BWI76_RS11350 at 2391.388 kb on - strand, within BWI76_RS11350 at 2391.547 kb on + strand, within BWI76_RS11350 at 2391.620 kb on - strand, within BWI76_RS11350 at 2391.740 kb on - strand, within BWI76_RS11350
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 2,389,183 - +0.4 2,389,230 - -0.1 2,389,232 + -1.6 2,389,346 + BWI76_RS11335 0.32 +0.6 2,389,581 - +0.9 2,389,613 + +0.2 2,389,748 - +1.0 2,390,229 + BWI76_RS11345 0.24 -1.6 2,390,595 + BWI76_RS11345 0.65 -0.5 2,390,605 + BWI76_RS11345 0.66 -1.9 2,390,859 + +2.0 2,391,025 - +0.1 2,391,135 + -0.1 2,391,175 - BWI76_RS11350 0.11 -1.2 2,391,383 - BWI76_RS11350 0.23 +0.1 2,391,388 - BWI76_RS11350 0.23 -1.3 2,391,547 + BWI76_RS11350 0.32 +0.0 2,391,620 - BWI76_RS11350 0.36 +0.5 2,391,740 - BWI76_RS11350 0.43 -1.7
Or see this region's nucleotide sequence