Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09085

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09075 and BWI76_RS09080 are separated by 152 nucleotidesBWI76_RS09080 and BWI76_RS09085 overlap by 1 nucleotidesBWI76_RS09085 and BWI76_RS09090 are separated by 177 nucleotides BWI76_RS09075: BWI76_RS09075 - pyruvate formate lyase-activating protein, at 1,916,043 to 1,916,942 _RS09075 BWI76_RS09080: BWI76_RS09080 - molybdopterin-synthase adenylyltransferase MoeB, at 1,917,095 to 1,917,850 _RS09080 BWI76_RS09085: BWI76_RS09085 - molybdopterin molybdenumtransferase MoeA, at 1,917,850 to 1,919,085 _RS09085 BWI76_RS09090: BWI76_RS09090 - beta-aspartyl-peptidase, at 1,919,263 to 1,920,204 _RS09090 Position (kb) 1917 1918 1919 1920Strain fitness (log2 ratio) -3 -2 -1 0 1at 1917.117 kb on + strandat 1917.343 kb on - strand, within BWI76_RS09080at 1917.670 kb on + strand, within BWI76_RS09080at 1917.818 kb on - strandat 1917.838 kb on + strandat 1917.865 kb on - strandat 1918.082 kb on - strand, within BWI76_RS09085at 1918.231 kb on + strand, within BWI76_RS09085at 1918.347 kb on - strand, within BWI76_RS09085at 1918.418 kb on + strand, within BWI76_RS09085at 1918.435 kb on + strand, within BWI76_RS09085at 1918.585 kb on - strand, within BWI76_RS09085at 1918.846 kb on + strand, within BWI76_RS09085at 1919.054 kb on - strandat 1919.139 kb on - strandat 1919.319 kb on + strandat 1919.343 kb on + strandat 1919.348 kb on - strandat 1919.353 kb on - strandat 1919.403 kb on - strand, within BWI76_RS09090at 1919.416 kb on - strand, within BWI76_RS09090at 1919.476 kb on - strand, within BWI76_RS09090at 1919.975 kb on - strand, within BWI76_RS09090at 1920.075 kb on + strand, within BWI76_RS09090

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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1,917,117 + -1.2
1,917,343 - BWI76_RS09080 0.33 -2.7
1,917,670 + BWI76_RS09080 0.76 +0.4
1,917,818 - -1.1
1,917,838 + -0.4
1,917,865 - +0.5
1,918,082 - BWI76_RS09085 0.19 -0.8
1,918,231 + BWI76_RS09085 0.31 -0.1
1,918,347 - BWI76_RS09085 0.40 -1.1
1,918,418 + BWI76_RS09085 0.46 -0.9
1,918,435 + BWI76_RS09085 0.47 -1.7
1,918,585 - BWI76_RS09085 0.59 -1.7
1,918,846 + BWI76_RS09085 0.81 -1.4
1,919,054 - -1.3
1,919,139 - -1.1
1,919,319 + -3.1
1,919,343 + -0.3
1,919,348 - -2.1
1,919,353 - -0.4
1,919,403 - BWI76_RS09090 0.15 +0.2
1,919,416 - BWI76_RS09090 0.16 +0.9
1,919,476 - BWI76_RS09090 0.23 +0.5
1,919,975 - BWI76_RS09090 0.76 -0.1
1,920,075 + BWI76_RS09090 0.86 -0.4

Or see this region's nucleotide sequence