Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08985

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08980 and BWI76_RS08985 are separated by 221 nucleotidesBWI76_RS08985 and BWI76_RS08990 are separated by 351 nucleotidesBWI76_RS08990 and BWI76_RS08995 are separated by 69 nucleotides BWI76_RS08980: BWI76_RS08980 - DNA starvation/stationary phase protection protein, at 1,892,519 to 1,893,022 _RS08980 BWI76_RS08985: BWI76_RS08985 - threonine/homoserine exporter RhtA, at 1,893,244 to 1,894,131 _RS08985 BWI76_RS08990: BWI76_RS08990 - outer membrane protein X, at 1,894,483 to 1,894,995 _RS08990 BWI76_RS08995: BWI76_RS08995 - sulfatase, at 1,895,065 to 1,896,642 _RS08995 Position (kb) 1893 1894 1895Strain fitness (log2 ratio) -1 0 1at 1892.449 kb on + strandat 1892.457 kb on - strandat 1892.524 kb on - strandat 1892.683 kb on - strand, within BWI76_RS08980at 1893.108 kb on + strandat 1893.299 kb on - strandat 1893.461 kb on + strand, within BWI76_RS08985at 1893.469 kb on - strand, within BWI76_RS08985at 1893.586 kb on + strand, within BWI76_RS08985at 1893.950 kb on + strand, within BWI76_RS08985at 1894.194 kb on - strandat 1894.288 kb on - strandat 1894.372 kb on + strandat 1894.441 kb on + strandat 1894.453 kb on + strandat 1894.507 kb on + strandat 1894.616 kb on - strand, within BWI76_RS08990at 1894.975 kb on + strandat 1895.102 kb on - strandat 1895.112 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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1,892,449 + -0.9
1,892,457 - -0.2
1,892,524 - +1.5
1,892,683 - BWI76_RS08980 0.33 -0.9
1,893,108 + +0.2
1,893,299 - -0.4
1,893,461 + BWI76_RS08985 0.24 -0.9
1,893,469 - BWI76_RS08985 0.25 -1.1
1,893,586 + BWI76_RS08985 0.39 -1.0
1,893,950 + BWI76_RS08985 0.80 -0.6
1,894,194 - -0.3
1,894,288 - -1.5
1,894,372 + +0.6
1,894,441 + -1.4
1,894,453 + -0.2
1,894,507 + +1.0
1,894,616 - BWI76_RS08990 0.26 +0.9
1,894,975 + +0.1
1,895,102 - +0.1
1,895,112 - -0.3

Or see this region's nucleotide sequence