Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07155 and BWI76_RS07160 are separated by 173 nucleotides BWI76_RS07160 and BWI76_RS07165 are separated by 9 nucleotides BWI76_RS07165 and BWI76_RS07170 are separated by 138 nucleotides
BWI76_RS07155: BWI76_RS07155 - fibronectin type III domain-containing protein, at 1,502,511 to 1,504,487
_RS07155
BWI76_RS07160: BWI76_RS07160 - MBL fold hydrolase, at 1,504,661 to 1,505,488
_RS07160
BWI76_RS07165: BWI76_RS07165 - hypothetical protein, at 1,505,498 to 1,506,040
_RS07165
BWI76_RS07170: BWI76_RS07170 - LysR family transcriptional regulator, at 1,506,179 to 1,507,054
_RS07170
Position (kb)
1504
1505
1506 Strain fitness (log2 ratio)
-2
-1
0
1 at 1503.777 kb on - strand, within BWI76_RS07155 at 1503.896 kb on + strand, within BWI76_RS07155 at 1503.902 kb on - strand, within BWI76_RS07155 at 1503.902 kb on - strand, within BWI76_RS07155 at 1503.964 kb on - strand, within BWI76_RS07155 at 1504.062 kb on + strand, within BWI76_RS07155 at 1504.079 kb on + strand, within BWI76_RS07155 at 1504.079 kb on + strand, within BWI76_RS07155 at 1504.087 kb on - strand, within BWI76_RS07155 at 1504.087 kb on - strand, within BWI76_RS07155 at 1504.385 kb on + strand at 1504.409 kb on + strand at 1504.449 kb on + strand at 1504.463 kb on + strand at 1504.587 kb on + strand at 1504.587 kb on + strand at 1504.633 kb on + strand at 1504.660 kb on - strand at 1504.675 kb on + strand at 1504.700 kb on + strand at 1504.712 kb on + strand at 1504.720 kb on - strand at 1504.799 kb on + strand, within BWI76_RS07160 at 1504.802 kb on - strand, within BWI76_RS07160 at 1504.802 kb on - strand, within BWI76_RS07160 at 1504.941 kb on - strand, within BWI76_RS07160 at 1505.289 kb on + strand, within BWI76_RS07160 at 1505.831 kb on - strand, within BWI76_RS07165 at 1505.861 kb on + strand, within BWI76_RS07165 at 1506.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 1,503,777 - BWI76_RS07155 0.64 +0.1 1,503,896 + BWI76_RS07155 0.70 +0.1 1,503,902 - BWI76_RS07155 0.70 +0.4 1,503,902 - BWI76_RS07155 0.70 -0.6 1,503,964 - BWI76_RS07155 0.73 -0.6 1,504,062 + BWI76_RS07155 0.78 -2.4 1,504,079 + BWI76_RS07155 0.79 +0.3 1,504,079 + BWI76_RS07155 0.79 -0.2 1,504,087 - BWI76_RS07155 0.80 +0.2 1,504,087 - BWI76_RS07155 0.80 +1.1 1,504,385 + -2.6 1,504,409 + -0.1 1,504,449 + -1.1 1,504,463 + -0.2 1,504,587 + -1.0 1,504,587 + -2.1 1,504,633 + -0.8 1,504,660 - -1.3 1,504,675 + -2.2 1,504,700 + -0.0 1,504,712 + +0.1 1,504,720 - +0.2 1,504,799 + BWI76_RS07160 0.17 -2.0 1,504,802 - BWI76_RS07160 0.17 -0.4 1,504,802 - BWI76_RS07160 0.17 -0.1 1,504,941 - BWI76_RS07160 0.34 -1.9 1,505,289 + BWI76_RS07160 0.76 -0.6 1,505,831 - BWI76_RS07165 0.61 +0.1 1,505,861 + BWI76_RS07165 0.67 -1.4 1,506,224 - -0.8
Or see this region's nucleotide sequence