Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02575 and BWI76_RS02580 are separated by 85 nucleotides BWI76_RS02580 and BWI76_RS02585 are separated by 45 nucleotides BWI76_RS02585 and BWI76_RS02590 are separated by 105 nucleotides
BWI76_RS02575: BWI76_RS02575 - exoribonuclease R, at 526,736 to 529,183
_RS02575
BWI76_RS02580: BWI76_RS02580 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase, at 529,269 to 530,000
_RS02580
BWI76_RS02585: BWI76_RS02585 - hypothetical protein, at 530,046 to 530,399
_RS02585
BWI76_RS02590: BWI76_RS02590 - isovaleryl-CoA dehydrogenase, at 530,505 to 532,142
_RS02590
Position (kb) 529
530
531 Strain fitness (log2 ratio) -2
-1
0
1 at 528.459 kb on + strand, within BWI76_RS02575 at 528.757 kb on - strand, within BWI76_RS02575 at 528.884 kb on - strand, within BWI76_RS02575 at 529.089 kb on - strand at 529.149 kb on - strand at 529.667 kb on + strand, within BWI76_RS02580 at 529.792 kb on + strand, within BWI76_RS02580 at 529.804 kb on + strand, within BWI76_RS02580 at 529.971 kb on - strand at 530.042 kb on - strand at 530.042 kb on - strand at 530.114 kb on - strand, within BWI76_RS02585 at 530.114 kb on - strand, within BWI76_RS02585 at 530.253 kb on + strand, within BWI76_RS02585 at 530.286 kb on - strand, within BWI76_RS02585 at 530.351 kb on + strand, within BWI76_RS02585 at 530.353 kb on + strand, within BWI76_RS02585 at 530.526 kb on - strand at 530.552 kb on - strand at 530.827 kb on + strand, within BWI76_RS02590 at 530.931 kb on - strand, within BWI76_RS02590
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 528,459 + BWI76_RS02575 0.70 -1.1 528,757 - BWI76_RS02575 0.83 -0.2 528,884 - BWI76_RS02575 0.88 +0.4 529,089 - +0.8 529,149 - +0.2 529,667 + BWI76_RS02580 0.54 -0.8 529,792 + BWI76_RS02580 0.71 -1.7 529,804 + BWI76_RS02580 0.73 -0.8 529,971 - -2.1 530,042 - -0.1 530,042 - -0.1 530,114 - BWI76_RS02585 0.19 -0.1 530,114 - BWI76_RS02585 0.19 +0.3 530,253 + BWI76_RS02585 0.58 +0.5 530,286 - BWI76_RS02585 0.68 +0.1 530,351 + BWI76_RS02585 0.86 -1.3 530,353 + BWI76_RS02585 0.87 +0.5 530,526 - -0.4 530,552 - +0.3 530,827 + BWI76_RS02590 0.20 -0.3 530,931 - BWI76_RS02590 0.26 +1.2
Or see this region's nucleotide sequence