Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00170

Experiment: K. M5a1 KLEB22 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00165 and BWI76_RS00170 are separated by 182 nucleotidesBWI76_RS00170 and BWI76_RS00175 are separated by 345 nucleotides BWI76_RS00165: BWI76_RS00165 - glutamine--fructose-6-phosphate aminotransferase, at 32,128 to 33,957 _RS00165 BWI76_RS00170: BWI76_RS00170 - bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase, at 34,140 to 35,510 _RS00170 BWI76_RS00175: BWI76_RS00175 - pectate disaccharide-lyase, at 35,856 to 38,027 _RS00175 Position (kb) 34 35 36Strain fitness (log2 ratio) -2 -1 0 1at 33.989 kb on - strandat 34.081 kb on + strandat 34.081 kb on + strandat 34.089 kb on - strandat 34.089 kb on - strandat 34.107 kb on - strandat 35.618 kb on - strandat 35.651 kb on + strandat 35.651 kb on + strandat 35.871 kb on + strandat 35.926 kb on - strandat 35.958 kb on - strandat 36.079 kb on - strand, within BWI76_RS00175at 36.181 kb on - strand, within BWI76_RS00175at 36.313 kb on - strand, within BWI76_RS00175

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10
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33,989 - +0.5
34,081 + +0.4
34,081 + +1.6
34,089 - +0.2
34,089 - +0.2
34,107 - -0.7
35,618 - +0.5
35,651 + -0.1
35,651 + +0.2
35,871 + +0.3
35,926 - +1.0
35,958 - -2.1
36,079 - BWI76_RS00175 0.10 -0.5
36,181 - BWI76_RS00175 0.15 +0.5
36,313 - BWI76_RS00175 0.21 +0.1

Or see this region's nucleotide sequence