Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26500 and BWI76_RS26505 overlap by 1 nucleotides BWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotides BWI76_RS26510 and BWI76_RS26515 overlap by 1 nucleotides BWI76_RS26515 and BWI76_RS26520 overlap by 1 nucleotides
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487
_RS26505
BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317
_RS26510
BWI76_RS26515: BWI76_RS26515 - nickel import ATP-binding protein NikD, at 5,488,317 to 5,489,078
_RS26515
BWI76_RS26520: BWI76_RS26520 - nickel import ATP-binding protein NikE, at 5,489,078 to 5,489,848
_RS26520
Position (kb) 5487
5488
5489 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5486.493 kb on - strand at 5486.522 kb on + strand at 5486.583 kb on - strand at 5487.167 kb on + strand, within BWI76_RS26505 at 5487.213 kb on + strand, within BWI76_RS26505 at 5487.226 kb on - strand, within BWI76_RS26505 at 5487.305 kb on + strand, within BWI76_RS26505 at 5487.386 kb on - strand, within BWI76_RS26505 at 5487.448 kb on + strand at 5487.663 kb on - strand, within BWI76_RS26510 at 5487.821 kb on + strand, within BWI76_RS26510 at 5487.855 kb on + strand, within BWI76_RS26510 at 5487.880 kb on + strand, within BWI76_RS26510 at 5487.883 kb on - strand, within BWI76_RS26510 at 5487.974 kb on + strand, within BWI76_RS26510 at 5488.014 kb on - strand, within BWI76_RS26510 at 5488.040 kb on + strand, within BWI76_RS26510 at 5488.194 kb on - strand, within BWI76_RS26510 at 5488.222 kb on - strand, within BWI76_RS26510 at 5488.246 kb on + strand at 5488.355 kb on + strand at 5488.588 kb on + strand, within BWI76_RS26515 at 5488.596 kb on - strand, within BWI76_RS26515 at 5488.796 kb on + strand, within BWI76_RS26515 at 5488.972 kb on + strand, within BWI76_RS26515 at 5488.980 kb on - strand, within BWI76_RS26515 at 5489.080 kb on - strand at 5489.089 kb on - strand at 5489.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 5,486,493 - -1.4 5,486,522 + +0.7 5,486,583 - -0.1 5,487,167 + BWI76_RS26505 0.66 +0.7 5,487,213 + BWI76_RS26505 0.71 +0.3 5,487,226 - BWI76_RS26505 0.72 +1.4 5,487,305 + BWI76_RS26505 0.81 +0.3 5,487,386 - BWI76_RS26505 0.89 +0.3 5,487,448 + -2.5 5,487,663 - BWI76_RS26510 0.21 -0.3 5,487,821 + BWI76_RS26510 0.40 -0.8 5,487,855 + BWI76_RS26510 0.44 -0.5 5,487,880 + BWI76_RS26510 0.47 -0.3 5,487,883 - BWI76_RS26510 0.48 -0.1 5,487,974 + BWI76_RS26510 0.59 +0.5 5,488,014 - BWI76_RS26510 0.64 +0.4 5,488,040 + BWI76_RS26510 0.67 +0.0 5,488,194 - BWI76_RS26510 0.85 -1.8 5,488,222 - BWI76_RS26510 0.88 -1.2 5,488,246 + +0.3 5,488,355 + +0.4 5,488,588 + BWI76_RS26515 0.36 +0.5 5,488,596 - BWI76_RS26515 0.37 +0.2 5,488,796 + BWI76_RS26515 0.63 +4.3 5,488,972 + BWI76_RS26515 0.86 -0.3 5,488,980 - BWI76_RS26515 0.87 +0.5 5,489,080 - -0.5 5,489,089 - +0.4 5,489,140 + +0.5
Or see this region's nucleotide sequence