Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12435 and BWI76_RS12440 are separated by 25 nucleotides BWI76_RS12440 and BWI76_RS12445 overlap by 10 nucleotides
BWI76_RS12435: BWI76_RS12435 - putative amino acid amidohydrolase, at 2,598,942 to 2,600,111
_RS12435
BWI76_RS12440: BWI76_RS12440 - MFS transporter, at 2,600,137 to 2,601,528
_RS12440
BWI76_RS12445: BWI76_RS12445 - phage shock protein operon transcriptional activator, at 2,601,519 to 2,602,493
_RS12445
Position (kb)
2600
2601
2602 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2599.137 kb on - strand, within BWI76_RS12435 at 2599.211 kb on + strand, within BWI76_RS12435 at 2599.219 kb on - strand, within BWI76_RS12435 at 2599.285 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.381 kb on - strand, within BWI76_RS12435 at 2599.657 kb on + strand, within BWI76_RS12435 at 2599.664 kb on + strand, within BWI76_RS12435 at 2599.809 kb on + strand, within BWI76_RS12435 at 2599.809 kb on + strand, within BWI76_RS12435 at 2599.817 kb on - strand, within BWI76_RS12435 at 2599.897 kb on + strand, within BWI76_RS12435 at 2600.149 kb on - strand at 2600.452 kb on - strand, within BWI76_RS12440 at 2600.454 kb on + strand, within BWI76_RS12440 at 2600.462 kb on - strand, within BWI76_RS12440 at 2600.564 kb on + strand, within BWI76_RS12440 at 2600.739 kb on - strand, within BWI76_RS12440 at 2600.821 kb on + strand, within BWI76_RS12440 at 2600.980 kb on - strand, within BWI76_RS12440 at 2601.066 kb on - strand, within BWI76_RS12440 at 2601.192 kb on + strand, within BWI76_RS12440 at 2601.304 kb on - strand, within BWI76_RS12440 at 2601.352 kb on - strand, within BWI76_RS12440 at 2601.411 kb on + strand at 2601.442 kb on - strand at 2601.473 kb on + strand at 2601.655 kb on - strand, within BWI76_RS12445 at 2601.837 kb on + strand, within BWI76_RS12445 at 2601.993 kb on + strand, within BWI76_RS12445 at 2602.265 kb on + strand, within BWI76_RS12445 at 2602.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 2,599,137 - BWI76_RS12435 0.17 -3.4 2,599,211 + BWI76_RS12435 0.23 -1.9 2,599,219 - BWI76_RS12435 0.24 +1.3 2,599,285 - BWI76_RS12435 0.29 +1.6 2,599,366 - BWI76_RS12435 0.36 +1.7 2,599,366 - BWI76_RS12435 0.36 +1.3 2,599,366 - BWI76_RS12435 0.36 +3.6 2,599,381 - BWI76_RS12435 0.38 +0.5 2,599,657 + BWI76_RS12435 0.61 +0.6 2,599,664 + BWI76_RS12435 0.62 -0.1 2,599,809 + BWI76_RS12435 0.74 -0.2 2,599,809 + BWI76_RS12435 0.74 -0.0 2,599,817 - BWI76_RS12435 0.75 +0.2 2,599,897 + BWI76_RS12435 0.82 +0.2 2,600,149 - -0.1 2,600,452 - BWI76_RS12440 0.23 -3.4 2,600,454 + BWI76_RS12440 0.23 -0.6 2,600,462 - BWI76_RS12440 0.23 -2.2 2,600,564 + BWI76_RS12440 0.31 -0.4 2,600,739 - BWI76_RS12440 0.43 -1.9 2,600,821 + BWI76_RS12440 0.49 -1.9 2,600,980 - BWI76_RS12440 0.61 +0.2 2,601,066 - BWI76_RS12440 0.67 -0.2 2,601,192 + BWI76_RS12440 0.76 -1.1 2,601,304 - BWI76_RS12440 0.84 +0.7 2,601,352 - BWI76_RS12440 0.87 -1.2 2,601,411 + +0.4 2,601,442 - +0.1 2,601,473 + -0.5 2,601,655 - BWI76_RS12445 0.14 -0.1 2,601,837 + BWI76_RS12445 0.33 -2.7 2,601,993 + BWI76_RS12445 0.49 -0.2 2,602,265 + BWI76_RS12445 0.77 +0.1 2,602,423 + -0.9
Or see this region's nucleotide sequence