Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10325 and BWI76_RS10330 are separated by 186 nucleotides BWI76_RS10330 and BWI76_RS10335 are separated by 148 nucleotides BWI76_RS10335 and BWI76_RS10340 are separated by 89 nucleotides
BWI76_RS10325: BWI76_RS10325 - AraC family transcriptional regulator, at 2,186,453 to 2,187,292
_RS10325
BWI76_RS10330: BWI76_RS10330 - TetR family transcriptional regulator, at 2,187,479 to 2,188,093
_RS10330
BWI76_RS10335: BWI76_RS10335 - tyrosine permease, at 2,188,242 to 2,189,405
_RS10335
BWI76_RS10340: BWI76_RS10340 - hypothetical protein, at 2,189,495 to 2,190,724
_RS10340
Position (kb)
2188
2189
2190 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2187.242 kb on - strand at 2187.305 kb on + strand at 2187.337 kb on + strand at 2187.348 kb on + strand at 2187.464 kb on + strand at 2187.470 kb on + strand at 2187.514 kb on - strand at 2187.691 kb on + strand, within BWI76_RS10330 at 2187.781 kb on + strand, within BWI76_RS10330 at 2187.815 kb on + strand, within BWI76_RS10330 at 2188.000 kb on + strand, within BWI76_RS10330 at 2188.079 kb on + strand at 2188.142 kb on + strand at 2188.142 kb on + strand at 2188.142 kb on + strand at 2188.142 kb on + strand at 2188.142 kb on + strand at 2188.142 kb on + strand at 2188.150 kb on - strand at 2188.150 kb on - strand at 2188.150 kb on - strand at 2188.150 kb on - strand at 2188.150 kb on - strand at 2188.234 kb on + strand at 2188.242 kb on - strand at 2188.270 kb on + strand at 2188.270 kb on + strand at 2188.273 kb on - strand at 2188.275 kb on + strand at 2188.278 kb on - strand at 2188.278 kb on - strand at 2188.278 kb on - strand at 2188.283 kb on - strand at 2188.356 kb on + strand at 2188.368 kb on - strand, within BWI76_RS10335 at 2188.395 kb on - strand, within BWI76_RS10335 at 2188.417 kb on + strand, within BWI76_RS10335 at 2188.417 kb on + strand, within BWI76_RS10335 at 2188.422 kb on + strand, within BWI76_RS10335 at 2188.422 kb on + strand, within BWI76_RS10335 at 2188.425 kb on - strand, within BWI76_RS10335 at 2188.430 kb on - strand, within BWI76_RS10335 at 2188.430 kb on - strand, within BWI76_RS10335 at 2188.430 kb on - strand, within BWI76_RS10335 at 2188.461 kb on + strand, within BWI76_RS10335 at 2188.461 kb on + strand, within BWI76_RS10335 at 2188.469 kb on - strand, within BWI76_RS10335 at 2188.500 kb on - strand, within BWI76_RS10335 at 2188.522 kb on + strand, within BWI76_RS10335 at 2188.530 kb on + strand, within BWI76_RS10335 at 2188.532 kb on + strand, within BWI76_RS10335 at 2188.539 kb on - strand, within BWI76_RS10335 at 2188.572 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.598 kb on + strand, within BWI76_RS10335 at 2188.606 kb on - strand, within BWI76_RS10335 at 2188.606 kb on - strand, within BWI76_RS10335 at 2188.616 kb on + strand, within BWI76_RS10335 at 2188.616 kb on - strand, within BWI76_RS10335 at 2188.616 kb on - strand, within BWI76_RS10335 at 2188.621 kb on - strand, within BWI76_RS10335 at 2188.711 kb on + strand, within BWI76_RS10335 at 2188.723 kb on + strand, within BWI76_RS10335 at 2188.723 kb on + strand, within BWI76_RS10335 at 2188.733 kb on + strand, within BWI76_RS10335 at 2188.897 kb on - strand, within BWI76_RS10335 at 2188.937 kb on + strand, within BWI76_RS10335 at 2188.937 kb on + strand, within BWI76_RS10335 at 2188.945 kb on - strand, within BWI76_RS10335 at 2189.017 kb on - strand, within BWI76_RS10335 at 2189.091 kb on + strand, within BWI76_RS10335 at 2189.618 kb on + strand, within BWI76_RS10340 at 2189.821 kb on + strand, within BWI76_RS10340 at 2190.142 kb on + strand, within BWI76_RS10340 at 2190.142 kb on + strand, within BWI76_RS10340 at 2190.152 kb on + strand, within BWI76_RS10340 at 2190.386 kb on + strand, within BWI76_RS10340
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 2,187,242 - +1.2 2,187,305 + -0.6 2,187,337 + +0.0 2,187,348 + +0.9 2,187,464 + +0.2 2,187,470 + +0.2 2,187,514 - +0.1 2,187,691 + BWI76_RS10330 0.34 +1.5 2,187,781 + BWI76_RS10330 0.49 +1.3 2,187,815 + BWI76_RS10330 0.55 +0.6 2,188,000 + BWI76_RS10330 0.85 -0.2 2,188,079 + +0.5 2,188,142 + -2.0 2,188,142 + +0.3 2,188,142 + +0.1 2,188,142 + -0.8 2,188,142 + +0.5 2,188,142 + -0.0 2,188,150 - +0.4 2,188,150 - -1.5 2,188,150 - -0.8 2,188,150 - -0.9 2,188,150 - -0.2 2,188,234 + +0.5 2,188,242 - -2.1 2,188,270 + +1.4 2,188,270 + -1.0 2,188,273 - +1.6 2,188,275 + +0.3 2,188,278 - -3.2 2,188,278 - +0.6 2,188,278 - +1.9 2,188,283 - -1.0 2,188,356 + -1.6 2,188,368 - BWI76_RS10335 0.11 -0.9 2,188,395 - BWI76_RS10335 0.13 -1.7 2,188,417 + BWI76_RS10335 0.15 +1.8 2,188,417 + BWI76_RS10335 0.15 +0.3 2,188,422 + BWI76_RS10335 0.15 +0.6 2,188,422 + BWI76_RS10335 0.15 +0.4 2,188,425 - BWI76_RS10335 0.16 +0.8 2,188,430 - BWI76_RS10335 0.16 -1.6 2,188,430 - BWI76_RS10335 0.16 -0.6 2,188,430 - BWI76_RS10335 0.16 +0.4 2,188,461 + BWI76_RS10335 0.19 -0.3 2,188,461 + BWI76_RS10335 0.19 +0.6 2,188,469 - BWI76_RS10335 0.20 +0.3 2,188,500 - BWI76_RS10335 0.22 +0.3 2,188,522 + BWI76_RS10335 0.24 -0.7 2,188,530 + BWI76_RS10335 0.25 +1.2 2,188,532 + BWI76_RS10335 0.25 +0.7 2,188,539 - BWI76_RS10335 0.26 +0.4 2,188,572 + BWI76_RS10335 0.28 -1.2 2,188,598 + BWI76_RS10335 0.31 +1.1 2,188,598 + BWI76_RS10335 0.31 +0.4 2,188,598 + BWI76_RS10335 0.31 -1.3 2,188,598 + BWI76_RS10335 0.31 +0.6 2,188,598 + BWI76_RS10335 0.31 +1.7 2,188,598 + BWI76_RS10335 0.31 +0.8 2,188,598 + BWI76_RS10335 0.31 +0.6 2,188,606 - BWI76_RS10335 0.31 -0.1 2,188,606 - BWI76_RS10335 0.31 +0.0 2,188,616 + BWI76_RS10335 0.32 +0.2 2,188,616 - BWI76_RS10335 0.32 -0.0 2,188,616 - BWI76_RS10335 0.32 -0.1 2,188,621 - BWI76_RS10335 0.33 -1.1 2,188,711 + BWI76_RS10335 0.40 +1.6 2,188,723 + BWI76_RS10335 0.41 +0.9 2,188,723 + BWI76_RS10335 0.41 +0.9 2,188,733 + BWI76_RS10335 0.42 +1.4 2,188,897 - BWI76_RS10335 0.56 +0.4 2,188,937 + BWI76_RS10335 0.60 -0.0 2,188,937 + BWI76_RS10335 0.60 -0.9 2,188,945 - BWI76_RS10335 0.60 +0.1 2,189,017 - BWI76_RS10335 0.67 -0.9 2,189,091 + BWI76_RS10335 0.73 -0.4 2,189,618 + BWI76_RS10340 0.10 +0.8 2,189,821 + BWI76_RS10340 0.27 -0.1 2,190,142 + BWI76_RS10340 0.53 +0.4 2,190,142 + BWI76_RS10340 0.53 +0.3 2,190,152 + BWI76_RS10340 0.53 +0.6 2,190,386 + BWI76_RS10340 0.72 +0.1
Or see this region's nucleotide sequence