Experiment: K. M5a1 KLEB22 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04585 and BWI76_RS04590 are separated by 10 nucleotides BWI76_RS04590 and BWI76_RS04595 are separated by 337 nucleotides
BWI76_RS04585: BWI76_RS04585 - L-arabinose isomerase, at 953,218 to 954,720
_RS04585
BWI76_RS04590: BWI76_RS04590 - ribulokinase, at 954,731 to 956,440
_RS04590
BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623
_RS04595
Position (kb) 954
955
956
957 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 953.743 kb on + strand, within BWI76_RS04585 at 953.916 kb on + strand, within BWI76_RS04585 at 953.924 kb on - strand, within BWI76_RS04585 at 953.965 kb on - strand, within BWI76_RS04585 at 954.053 kb on + strand, within BWI76_RS04585 at 954.090 kb on + strand, within BWI76_RS04585 at 954.092 kb on + strand, within BWI76_RS04585 at 954.097 kb on + strand, within BWI76_RS04585 at 954.267 kb on + strand, within BWI76_RS04585 at 954.275 kb on - strand, within BWI76_RS04585 at 954.641 kb on + strand at 954.662 kb on - strand at 954.972 kb on + strand, within BWI76_RS04590 at 954.977 kb on + strand, within BWI76_RS04590 at 954.982 kb on + strand, within BWI76_RS04590 at 955.036 kb on - strand, within BWI76_RS04590 at 955.114 kb on + strand, within BWI76_RS04590 at 955.187 kb on + strand, within BWI76_RS04590 at 955.307 kb on - strand, within BWI76_RS04590 at 955.335 kb on + strand, within BWI76_RS04590 at 955.335 kb on + strand, within BWI76_RS04590 at 955.372 kb on - strand, within BWI76_RS04590 at 955.512 kb on - strand, within BWI76_RS04590 at 955.666 kb on - strand, within BWI76_RS04590 at 955.726 kb on + strand, within BWI76_RS04590 at 955.911 kb on - strand, within BWI76_RS04590 at 956.018 kb on - strand, within BWI76_RS04590 at 956.029 kb on + strand, within BWI76_RS04590 at 956.139 kb on + strand, within BWI76_RS04590 at 956.223 kb on - strand, within BWI76_RS04590 at 956.310 kb on + strand at 956.352 kb on - strand at 956.484 kb on + strand at 956.487 kb on - strand at 956.539 kb on - strand at 956.749 kb on + strand at 956.908 kb on + strand, within BWI76_RS04595 at 956.916 kb on - strand, within BWI76_RS04595 at 956.921 kb on - strand, within BWI76_RS04595 at 956.966 kb on + strand, within BWI76_RS04595 at 957.063 kb on + strand, within BWI76_RS04595 at 957.197 kb on - strand, within BWI76_RS04595 at 957.217 kb on + strand, within BWI76_RS04595 at 957.225 kb on - strand, within BWI76_RS04595 at 957.302 kb on + strand, within BWI76_RS04595
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 10 remove 953,743 + BWI76_RS04585 0.35 +0.7 953,916 + BWI76_RS04585 0.46 +0.5 953,924 - BWI76_RS04585 0.47 -0.5 953,965 - BWI76_RS04585 0.50 -3.0 954,053 + BWI76_RS04585 0.56 -0.2 954,090 + BWI76_RS04585 0.58 +1.2 954,092 + BWI76_RS04585 0.58 -0.2 954,097 + BWI76_RS04585 0.58 +0.7 954,267 + BWI76_RS04585 0.70 +1.2 954,275 - BWI76_RS04585 0.70 +0.0 954,641 + -0.7 954,662 - -0.4 954,972 + BWI76_RS04590 0.14 -0.4 954,977 + BWI76_RS04590 0.14 -1.7 954,982 + BWI76_RS04590 0.15 -0.2 955,036 - BWI76_RS04590 0.18 +0.3 955,114 + BWI76_RS04590 0.22 -0.4 955,187 + BWI76_RS04590 0.27 -0.1 955,307 - BWI76_RS04590 0.34 +1.2 955,335 + BWI76_RS04590 0.35 -0.2 955,335 + BWI76_RS04590 0.35 +0.5 955,372 - BWI76_RS04590 0.37 +0.3 955,512 - BWI76_RS04590 0.46 +0.3 955,666 - BWI76_RS04590 0.55 +0.5 955,726 + BWI76_RS04590 0.58 +0.7 955,911 - BWI76_RS04590 0.69 +0.3 956,018 - BWI76_RS04590 0.75 -0.1 956,029 + BWI76_RS04590 0.76 +0.7 956,139 + BWI76_RS04590 0.82 +0.1 956,223 - BWI76_RS04590 0.87 +0.5 956,310 + -0.6 956,352 - +0.1 956,484 + -0.2 956,487 - +1.5 956,539 - +0.0 956,749 + +0.1 956,908 + BWI76_RS04595 0.15 -0.5 956,916 - BWI76_RS04595 0.16 -0.5 956,921 - BWI76_RS04595 0.17 +0.6 956,966 + BWI76_RS04595 0.22 -0.0 957,063 + BWI76_RS04595 0.34 +3.6 957,197 - BWI76_RS04595 0.50 +0.4 957,217 + BWI76_RS04595 0.52 -0.5 957,225 - BWI76_RS04595 0.53 -2.3 957,302 + BWI76_RS04595 0.62 +0.5
Or see this region's nucleotide sequence