Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26515

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotidesBWI76_RS26510 and BWI76_RS26515 overlap by 1 nucleotidesBWI76_RS26515 and BWI76_RS26520 overlap by 1 nucleotidesBWI76_RS26520 and BWI76_RS26525 are separated by 5 nucleotides BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487 _RS26505 BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317 _RS26510 BWI76_RS26515: BWI76_RS26515 - nickel import ATP-binding protein NikD, at 5,488,317 to 5,489,078 _RS26515 BWI76_RS26520: BWI76_RS26520 - nickel import ATP-binding protein NikE, at 5,489,078 to 5,489,848 _RS26520 BWI76_RS26525: BWI76_RS26525 - nickel-responsive transcriptional regulator NikR, at 5,489,854 to 5,490,270 _RS26525 Position (kb) 5488 5489 5490Strain fitness (log2 ratio) -1 0 1at 5487.386 kb on - strand, within BWI76_RS26505at 5487.448 kb on + strandat 5487.663 kb on - strand, within BWI76_RS26510at 5487.821 kb on + strand, within BWI76_RS26510at 5487.855 kb on + strand, within BWI76_RS26510at 5487.880 kb on + strand, within BWI76_RS26510at 5487.883 kb on - strand, within BWI76_RS26510at 5487.974 kb on + strand, within BWI76_RS26510at 5488.014 kb on - strand, within BWI76_RS26510at 5488.040 kb on + strand, within BWI76_RS26510at 5488.194 kb on - strand, within BWI76_RS26510at 5488.222 kb on - strand, within BWI76_RS26510at 5488.246 kb on + strandat 5488.355 kb on + strandat 5488.588 kb on + strand, within BWI76_RS26515at 5488.596 kb on - strand, within BWI76_RS26515at 5488.796 kb on + strand, within BWI76_RS26515at 5488.972 kb on + strand, within BWI76_RS26515at 5488.980 kb on - strand, within BWI76_RS26515at 5489.080 kb on - strandat 5489.089 kb on - strandat 5489.140 kb on + strandat 5489.338 kb on - strand, within BWI76_RS26520at 5489.343 kb on + strand, within BWI76_RS26520at 5489.401 kb on - strand, within BWI76_RS26520at 5489.443 kb on - strand, within BWI76_RS26520at 5489.460 kb on - strand, within BWI76_RS26520at 5489.493 kb on - strand, within BWI76_RS26520at 5489.493 kb on - strand, within BWI76_RS26520at 5489.526 kb on - strand, within BWI76_RS26520at 5489.875 kb on - strandat 5489.914 kb on - strand, within BWI76_RS26525at 5489.956 kb on + strand, within BWI76_RS26525at 5490.001 kb on + strand, within BWI76_RS26525at 5490.001 kb on + strand, within BWI76_RS26525at 5490.009 kb on - strand, within BWI76_RS26525at 5490.009 kb on - strand, within BWI76_RS26525

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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5,487,386 - BWI76_RS26505 0.89 -0.3
5,487,448 + +0.0
5,487,663 - BWI76_RS26510 0.21 -0.2
5,487,821 + BWI76_RS26510 0.40 -0.3
5,487,855 + BWI76_RS26510 0.44 -0.3
5,487,880 + BWI76_RS26510 0.47 -0.4
5,487,883 - BWI76_RS26510 0.48 +0.2
5,487,974 + BWI76_RS26510 0.59 +0.8
5,488,014 - BWI76_RS26510 0.64 +1.5
5,488,040 + BWI76_RS26510 0.67 +0.2
5,488,194 - BWI76_RS26510 0.85 -0.1
5,488,222 - BWI76_RS26510 0.88 +0.5
5,488,246 + +0.0
5,488,355 + -0.1
5,488,588 + BWI76_RS26515 0.36 -0.9
5,488,596 - BWI76_RS26515 0.37 -1.0
5,488,796 + BWI76_RS26515 0.63 +0.8
5,488,972 + BWI76_RS26515 0.86 +0.1
5,488,980 - BWI76_RS26515 0.87 -0.6
5,489,080 - -0.3
5,489,089 - +0.2
5,489,140 + -0.5
5,489,338 - BWI76_RS26520 0.34 +0.2
5,489,343 + BWI76_RS26520 0.34 +0.2
5,489,401 - BWI76_RS26520 0.42 +0.3
5,489,443 - BWI76_RS26520 0.47 +0.4
5,489,460 - BWI76_RS26520 0.50 +0.7
5,489,493 - BWI76_RS26520 0.54 -0.7
5,489,493 - BWI76_RS26520 0.54 -1.0
5,489,526 - BWI76_RS26520 0.58 +0.2
5,489,875 - -0.3
5,489,914 - BWI76_RS26525 0.14 +0.4
5,489,956 + BWI76_RS26525 0.24 -0.2
5,490,001 + BWI76_RS26525 0.35 -1.0
5,490,001 + BWI76_RS26525 0.35 -0.6
5,490,009 - BWI76_RS26525 0.37 +1.3
5,490,009 - BWI76_RS26525 0.37 +0.1

Or see this region's nucleotide sequence