Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20720 and BWI76_RS20725 overlap by 14 nucleotides BWI76_RS20725 and BWI76_RS20730 are separated by 21 nucleotides BWI76_RS20730 and BWI76_RS20735 overlap by 8 nucleotides BWI76_RS20735 and BWI76_RS20740 overlap by 1 nucleotides
BWI76_RS20720: BWI76_RS20720 - hypothetical protein, at 4,333,530 to 4,333,979
_RS20720
BWI76_RS20725: BWI76_RS20725 - GNAT family acetyltransferase, at 4,333,966 to 4,334,391
_RS20725
BWI76_RS20730: BWI76_RS20730 - hypothetical protein, at 4,334,413 to 4,334,610
_RS20730
BWI76_RS20735: BWI76_RS20735 - N-acetylmuramoyl-L-alanine amidase, at 4,334,603 to 4,335,478
_RS20735
BWI76_RS20740: BWI76_RS20740 - coproporphyrinogen III oxidase, at 4,335,478 to 4,336,377
_RS20740
Position (kb) 4334
4335
4336 Strain fitness (log2 ratio) -1
0
1 at 4333.842 kb on - strand, within BWI76_RS20720 at 4333.901 kb on - strand, within BWI76_RS20720 at 4334.034 kb on - strand, within BWI76_RS20725 at 4334.262 kb on + strand, within BWI76_RS20725 at 4334.325 kb on - strand, within BWI76_RS20725 at 4334.337 kb on + strand, within BWI76_RS20725 at 4334.345 kb on + strand, within BWI76_RS20725 at 4334.394 kb on - strand at 4334.403 kb on + strand at 4334.403 kb on - strand at 4334.420 kb on - strand at 4334.572 kb on - strand, within BWI76_RS20730 at 4334.904 kb on + strand, within BWI76_RS20735 at 4334.945 kb on - strand, within BWI76_RS20735 at 4335.154 kb on - strand, within BWI76_RS20735 at 4335.331 kb on - strand, within BWI76_RS20735 at 4335.498 kb on + strand at 4335.498 kb on + strand at 4335.710 kb on + strand, within BWI76_RS20740 at 4335.723 kb on - strand, within BWI76_RS20740 at 4335.781 kb on - strand, within BWI76_RS20740 at 4335.938 kb on - strand, within BWI76_RS20740 at 4335.982 kb on + strand, within BWI76_RS20740 at 4336.155 kb on - strand, within BWI76_RS20740
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 4,333,842 - BWI76_RS20720 0.69 +0.9 4,333,901 - BWI76_RS20720 0.82 +1.2 4,334,034 - BWI76_RS20725 0.16 -0.9 4,334,262 + BWI76_RS20725 0.69 -0.3 4,334,325 - BWI76_RS20725 0.84 +0.9 4,334,337 + BWI76_RS20725 0.87 -0.9 4,334,345 + BWI76_RS20725 0.89 +0.3 4,334,394 - +0.3 4,334,403 + -0.9 4,334,403 - -0.4 4,334,420 - -0.4 4,334,572 - BWI76_RS20730 0.80 -1.4 4,334,904 + BWI76_RS20735 0.34 -0.1 4,334,945 - BWI76_RS20735 0.39 -0.5 4,335,154 - BWI76_RS20735 0.63 -1.1 4,335,331 - BWI76_RS20735 0.83 -0.3 4,335,498 + -0.0 4,335,498 + +0.7 4,335,710 + BWI76_RS20740 0.26 +0.9 4,335,723 - BWI76_RS20740 0.27 -0.1 4,335,781 - BWI76_RS20740 0.34 -0.4 4,335,938 - BWI76_RS20740 0.51 +0.2 4,335,982 + BWI76_RS20740 0.56 -0.0 4,336,155 - BWI76_RS20740 0.75 +0.5
Or see this region's nucleotide sequence