Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14390 and BWI76_RS14395 are separated by 517 nucleotides BWI76_RS14395 and BWI76_RS14400 are separated by 110 nucleotides BWI76_RS14400 and BWI76_RS14405 are separated by 120 nucleotides BWI76_RS14405 and BWI76_RS14410 are separated by 91 nucleotides BWI76_RS14410 and BWI76_RS14415 are separated by 118 nucleotides
BWI76_RS14390: BWI76_RS14390 - hypothetical protein, at 3,006,987 to 3,007,862
_RS14390
BWI76_RS14395: BWI76_RS14395 - hypothetical protein, at 3,008,380 to 3,008,517
_RS14395
BWI76_RS14400: BWI76_RS14400 - ISNCY family transposase, at 3,008,628 to 3,009,198
_RS14400
BWI76_RS14405: BWI76_RS14405 - steroid Delta-isomerase, at 3,009,319 to 3,009,672
_RS14405
BWI76_RS14410: BWI76_RS14410 - hypothetical protein, at 3,009,764 to 3,009,967
_RS14410
BWI76_RS14415: BWI76_RS14415 - transcriptional regulator, TetR family protein, at 3,010,086 to 3,010,682
_RS14415
Position (kb)
3008
3009
3010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3007.860 kb on + strand at 3008.043 kb on + strand at 3008.043 kb on + strand at 3008.045 kb on + strand at 3008.045 kb on + strand at 3008.077 kb on - strand at 3008.160 kb on - strand at 3008.247 kb on + strand at 3008.260 kb on + strand at 3008.260 kb on + strand at 3008.605 kb on - strand at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.959 kb on - strand, within BWI76_RS14400 at 3009.019 kb on - strand, within BWI76_RS14400 at 3009.084 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.251 kb on + strand at 3009.264 kb on - strand at 3009.766 kb on - strand at 3009.812 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.848 kb on + strand, within BWI76_RS14410 at 3009.856 kb on - strand at 3009.962 kb on + strand at 3009.970 kb on - strand at 3009.970 kb on - strand at 3009.986 kb on + strand at 3009.989 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3010.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 3,007,860 + +0.6 3,008,043 + -0.2 3,008,043 + -0.7 3,008,045 + +2.4 3,008,045 + +0.1 3,008,077 - -1.0 3,008,160 - +0.6 3,008,247 + +0.6 3,008,260 + +0.6 3,008,260 + +1.6 3,008,605 - -0.4 3,008,909 + BWI76_RS14400 0.49 -0.0 3,008,909 + BWI76_RS14400 0.49 +0.9 3,008,959 - BWI76_RS14400 0.58 -0.7 3,009,019 - BWI76_RS14400 0.68 -0.8 3,009,084 + BWI76_RS14400 0.80 -2.8 3,009,096 + BWI76_RS14400 0.82 -0.5 3,009,096 + BWI76_RS14400 0.82 -1.2 3,009,096 + BWI76_RS14400 0.82 -0.3 3,009,096 + BWI76_RS14400 0.82 +1.6 3,009,096 + BWI76_RS14400 0.82 -1.4 3,009,130 - BWI76_RS14400 0.88 -0.6 3,009,130 - BWI76_RS14400 0.88 +1.1 3,009,178 + -0.2 3,009,178 + -0.1 3,009,178 + -0.1 3,009,251 + +3.2 3,009,264 - +0.1 3,009,766 - +0.6 3,009,812 - BWI76_RS14410 0.24 +0.6 3,009,822 - BWI76_RS14410 0.28 +1.6 3,009,822 - BWI76_RS14410 0.28 -2.0 3,009,822 - BWI76_RS14410 0.28 +1.1 3,009,848 + BWI76_RS14410 0.41 -0.6 3,009,856 - +0.3 3,009,962 + -0.7 3,009,970 - +1.4 3,009,970 - +1.2 3,009,986 + -0.0 3,009,989 - +1.1 3,009,994 - +1.3 3,009,994 - -0.4 3,009,994 - -1.0 3,009,994 - +0.8 3,010,128 + -0.2
Or see this region's nucleotide sequence