Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13310

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13305 and BWI76_RS13310 are separated by 270 nucleotidesBWI76_RS13310 and BWI76_RS13315 are separated by 75 nucleotidesBWI76_RS13315 and BWI76_RS13320 are separated by 95 nucleotides BWI76_RS13305: BWI76_RS13305 - hydrolase, at 2,774,665 to 2,775,288 _RS13305 BWI76_RS13310: BWI76_RS13310 - glucan biosynthesis protein D, at 2,775,559 to 2,777,214 _RS13310 BWI76_RS13315: BWI76_RS13315 - 50S ribosomal protein L7/L12-serine acetyltransferase, at 2,777,290 to 2,777,874 _RS13315 BWI76_RS13320: BWI76_RS13320 - putative LpxA-like enzyme, at 2,777,970 to 2,778,950 _RS13320 Position (kb) 2775 2776 2777 2778Strain fitness (log2 ratio) -2 -1 0 1at 2775.271 kb on - strandat 2775.312 kb on - strandat 2775.448 kb on - strandat 2775.795 kb on + strand, within BWI76_RS13310at 2775.967 kb on - strand, within BWI76_RS13310at 2776.020 kb on - strand, within BWI76_RS13310at 2776.116 kb on + strand, within BWI76_RS13310at 2776.276 kb on - strand, within BWI76_RS13310at 2776.573 kb on - strand, within BWI76_RS13310at 2777.103 kb on - strandat 2777.126 kb on - strandat 2777.128 kb on - strandat 2777.367 kb on - strand, within BWI76_RS13315

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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2,775,271 - -2.5
2,775,312 - +0.3
2,775,448 - +0.4
2,775,795 + BWI76_RS13310 0.14 -0.9
2,775,967 - BWI76_RS13310 0.25 -0.9
2,776,020 - BWI76_RS13310 0.28 -0.6
2,776,116 + BWI76_RS13310 0.34 +0.4
2,776,276 - BWI76_RS13310 0.43 -0.6
2,776,573 - BWI76_RS13310 0.61 -1.1
2,777,103 - -0.0
2,777,126 - +0.2
2,777,128 - -0.2
2,777,367 - BWI76_RS13315 0.13 +0.5

Or see this region's nucleotide sequence