Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10685

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10680 and BWI76_RS10685 are separated by 103 nucleotidesBWI76_RS10685 and BWI76_RS10690 overlap by 4 nucleotides BWI76_RS10680: BWI76_RS10680 - tartrate dehydrogenase, at 2,256,672 to 2,257,790 _RS10680 BWI76_RS10685: BWI76_RS10685 - LysR family transcriptional regulator, at 2,257,894 to 2,258,850 _RS10685 BWI76_RS10690: BWI76_RS10690 - gamma-glutamylputrescine oxidoreductase, at 2,258,847 to 2,260,127 _RS10690 Position (kb) 2257 2258 2259Strain fitness (log2 ratio) -1 0 1at 2257.058 kb on + strand, within BWI76_RS10680at 2257.097 kb on + strand, within BWI76_RS10680at 2257.667 kb on + strand, within BWI76_RS10680at 2257.928 kb on + strandat 2257.928 kb on + strandat 2258.359 kb on - strand, within BWI76_RS10685at 2258.458 kb on + strand, within BWI76_RS10685at 2258.466 kb on - strand, within BWI76_RS10685at 2258.466 kb on - strand, within BWI76_RS10685at 2258.466 kb on - strand, within BWI76_RS10685at 2258.537 kb on + strand, within BWI76_RS10685at 2259.014 kb on + strand, within BWI76_RS10690at 2259.037 kb on + strand, within BWI76_RS10690at 2259.456 kb on - strand, within BWI76_RS10690at 2259.729 kb on - strand, within BWI76_RS10690at 2259.731 kb on - strand, within BWI76_RS10690at 2259.828 kb on + strand, within BWI76_RS10690

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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2,257,058 + BWI76_RS10680 0.34 +0.5
2,257,097 + BWI76_RS10680 0.38 -1.6
2,257,667 + BWI76_RS10680 0.89 +0.2
2,257,928 + +0.3
2,257,928 + -0.3
2,258,359 - BWI76_RS10685 0.49 -1.4
2,258,458 + BWI76_RS10685 0.59 -0.7
2,258,466 - BWI76_RS10685 0.60 -0.6
2,258,466 - BWI76_RS10685 0.60 -0.8
2,258,466 - BWI76_RS10685 0.60 -1.0
2,258,537 + BWI76_RS10685 0.67 +0.9
2,259,014 + BWI76_RS10690 0.13 +0.5
2,259,037 + BWI76_RS10690 0.15 -1.6
2,259,456 - BWI76_RS10690 0.48 +1.4
2,259,729 - BWI76_RS10690 0.69 +0.0
2,259,731 - BWI76_RS10690 0.69 -1.0
2,259,828 + BWI76_RS10690 0.77 -0.6

Or see this region's nucleotide sequence