Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09140

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09135 and BWI76_RS09140 are separated by 2 nucleotidesBWI76_RS09140 and BWI76_RS09145 are separated by 257 nucleotides BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073 _RS09135 BWI76_RS09140: BWI76_RS09140 - glutathione S-transferase, at 1,931,076 to 1,931,702 _RS09140 BWI76_RS09145: BWI76_RS09145 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,931,960 to 1,933,162 _RS09145 Position (kb) 1931 1932Strain fitness (log2 ratio) -2 -1 0 1 2at 1930.520 kb on - strand, within BWI76_RS09135at 1930.603 kb on + strand, within BWI76_RS09135at 1930.603 kb on + strand, within BWI76_RS09135at 1930.611 kb on - strand, within BWI76_RS09135at 1930.730 kb on + strand, within BWI76_RS09135at 1931.042 kb on - strandat 1931.229 kb on + strand, within BWI76_RS09140at 1931.268 kb on + strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.400 kb on - strand, within BWI76_RS09140at 1931.881 kb on + strandat 1932.218 kb on - strand, within BWI76_RS09145at 1932.451 kb on + strand, within BWI76_RS09145at 1932.507 kb on - strand, within BWI76_RS09145

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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1,930,520 - BWI76_RS09135 0.50 +0.6
1,930,603 + BWI76_RS09135 0.58 +0.2
1,930,603 + BWI76_RS09135 0.58 +0.7
1,930,611 - BWI76_RS09135 0.58 +0.1
1,930,730 + BWI76_RS09135 0.69 -0.6
1,931,042 - +0.6
1,931,229 + BWI76_RS09140 0.24 -0.1
1,931,268 + BWI76_RS09140 0.31 -1.4
1,931,352 - BWI76_RS09140 0.44 -0.0
1,931,352 - BWI76_RS09140 0.44 -2.1
1,931,400 - BWI76_RS09140 0.52 -0.8
1,931,881 + +0.5
1,932,218 - BWI76_RS09145 0.21 +0.2
1,932,451 + BWI76_RS09145 0.41 +0.3
1,932,507 - BWI76_RS09145 0.45 +1.8

Or see this region's nucleotide sequence