Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03415 and BWI76_RS03420 are separated by 136 nucleotides BWI76_RS03420 and BWI76_RS03425 overlap by 14 nucleotides BWI76_RS03425 and BWI76_RS03430 overlap by 7 nucleotides BWI76_RS03430 and BWI76_RS03435 are separated by 23 nucleotides
BWI76_RS03415: BWI76_RS03415 - LacI family transcriptional regulator, at 703,052 to 704,080
_RS03415
BWI76_RS03420: BWI76_RS03420 - hypothetical protein, at 704,217 to 704,648
_RS03420
BWI76_RS03425: BWI76_RS03425 - dihydrodipicolinate synthase family protein, at 704,635 to 705,546
_RS03425
BWI76_RS03430: BWI76_RS03430 - short-chain dehydrogenase, at 705,540 to 706,367
_RS03430
BWI76_RS03435: BWI76_RS03435 - mandelate racemase/muconate lactonizing protein, at 706,391 to 707,494
_RS03435
Position (kb) 704
705
706 Strain fitness (log2 ratio) -1
0
1 at 703.805 kb on + strand, within BWI76_RS03415 at 703.953 kb on + strand, within BWI76_RS03415 at 704.006 kb on - strand at 704.034 kb on + strand at 704.042 kb on - strand at 704.208 kb on - strand at 704.243 kb on - strand at 704.626 kb on - strand at 704.626 kb on - strand at 704.673 kb on + strand at 704.862 kb on + strand, within BWI76_RS03425 at 704.912 kb on - strand, within BWI76_RS03425 at 705.065 kb on + strand, within BWI76_RS03425 at 705.184 kb on - strand, within BWI76_RS03425 at 705.249 kb on - strand, within BWI76_RS03425 at 705.262 kb on - strand, within BWI76_RS03425 at 705.420 kb on - strand, within BWI76_RS03425 at 705.662 kb on + strand, within BWI76_RS03430 at 705.698 kb on + strand, within BWI76_RS03430 at 706.000 kb on - strand, within BWI76_RS03430 at 706.062 kb on + strand, within BWI76_RS03430 at 706.065 kb on - strand, within BWI76_RS03430 at 706.128 kb on + strand, within BWI76_RS03430 at 706.187 kb on - strand, within BWI76_RS03430 at 706.280 kb on - strand, within BWI76_RS03430 at 706.290 kb on - strand at 706.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 703,805 + BWI76_RS03415 0.73 +0.8 703,953 + BWI76_RS03415 0.88 +0.4 704,006 - +0.3 704,034 + +1.1 704,042 - +0.3 704,208 - +1.1 704,243 - +0.6 704,626 - +0.1 704,626 - -0.8 704,673 + +0.5 704,862 + BWI76_RS03425 0.25 -0.5 704,912 - BWI76_RS03425 0.30 -0.2 705,065 + BWI76_RS03425 0.47 -1.0 705,184 - BWI76_RS03425 0.60 -1.3 705,249 - BWI76_RS03425 0.67 -1.2 705,262 - BWI76_RS03425 0.69 -0.1 705,420 - BWI76_RS03425 0.86 -0.3 705,662 + BWI76_RS03430 0.15 +0.0 705,698 + BWI76_RS03430 0.19 +0.8 706,000 - BWI76_RS03430 0.56 -0.7 706,062 + BWI76_RS03430 0.63 -0.8 706,065 - BWI76_RS03430 0.63 +0.1 706,128 + BWI76_RS03430 0.71 +1.7 706,187 - BWI76_RS03430 0.78 -1.1 706,280 - BWI76_RS03430 0.89 +0.2 706,290 - -0.8 706,305 - -0.0
Or see this region's nucleotide sequence