Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18000 and BWI76_RS18005 are separated by 115 nucleotides BWI76_RS18005 and BWI76_RS18010 are separated by 97 nucleotides BWI76_RS18010 and BWI76_RS18015 overlap by 4 nucleotides
BWI76_RS18000: BWI76_RS18000 - 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF, at 3,741,442 to 3,742,917
_RS18000
BWI76_RS18005: BWI76_RS18005 - hypothetical protein, at 3,743,033 to 3,743,272
_RS18005
BWI76_RS18010: BWI76_RS18010 - hypothetical protein, at 3,743,370 to 3,743,561
_RS18010
BWI76_RS18015: BWI76_RS18015 - serine/threonine-protein phosphatase, at 3,743,558 to 3,744,211
_RS18015
Position (kb)
3743
3744 Strain fitness (log2 ratio)
-2
-1
0
1 at 3742.331 kb on - strand, within BWI76_RS18000 at 3742.387 kb on + strand, within BWI76_RS18000 at 3742.530 kb on + strand, within BWI76_RS18000 at 3742.744 kb on + strand, within BWI76_RS18000 at 3742.801 kb on + strand at 3743.072 kb on + strand, within BWI76_RS18005 at 3743.084 kb on - strand, within BWI76_RS18005 at 3743.084 kb on - strand, within BWI76_RS18005 at 3743.096 kb on + strand, within BWI76_RS18005 at 3743.201 kb on + strand, within BWI76_RS18005 at 3743.336 kb on - strand at 3743.336 kb on - strand at 3743.385 kb on - strand at 3743.659 kb on + strand, within BWI76_RS18015 at 3743.766 kb on - strand, within BWI76_RS18015 at 3743.819 kb on + strand, within BWI76_RS18015 at 3743.829 kb on - strand, within BWI76_RS18015 at 3744.070 kb on - strand, within BWI76_RS18015 at 3744.092 kb on + strand, within BWI76_RS18015 at 3744.103 kb on + strand, within BWI76_RS18015 at 3744.103 kb on + strand, within BWI76_RS18015 at 3744.111 kb on - strand, within BWI76_RS18015 at 3744.111 kb on - strand, within BWI76_RS18015 at 3744.111 kb on - strand, within BWI76_RS18015 at 3744.113 kb on + strand, within BWI76_RS18015 at 3744.132 kb on - strand, within BWI76_RS18015 at 3744.139 kb on - strand, within BWI76_RS18015 at 3744.144 kb on + strand, within BWI76_RS18015 at 3744.157 kb on - strand at 3744.157 kb on - strand at 3744.184 kb on + strand at 3744.192 kb on + strand at 3744.192 kb on + strand at 3744.258 kb on + strand at 3744.259 kb on + strand at 3744.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 3,742,331 - BWI76_RS18000 0.60 +0.3 3,742,387 + BWI76_RS18000 0.64 +0.4 3,742,530 + BWI76_RS18000 0.74 -2.0 3,742,744 + BWI76_RS18000 0.88 -0.1 3,742,801 + +0.0 3,743,072 + BWI76_RS18005 0.16 -0.6 3,743,084 - BWI76_RS18005 0.21 +0.3 3,743,084 - BWI76_RS18005 0.21 -1.1 3,743,096 + BWI76_RS18005 0.26 -1.2 3,743,201 + BWI76_RS18005 0.70 -0.2 3,743,336 - +0.2 3,743,336 - -0.2 3,743,385 - +0.4 3,743,659 + BWI76_RS18015 0.15 +0.8 3,743,766 - BWI76_RS18015 0.32 +0.5 3,743,819 + BWI76_RS18015 0.40 +0.1 3,743,829 - BWI76_RS18015 0.41 -0.3 3,744,070 - BWI76_RS18015 0.78 -0.0 3,744,092 + BWI76_RS18015 0.82 +1.4 3,744,103 + BWI76_RS18015 0.83 +0.0 3,744,103 + BWI76_RS18015 0.83 +0.4 3,744,111 - BWI76_RS18015 0.85 -0.0 3,744,111 - BWI76_RS18015 0.85 +0.6 3,744,111 - BWI76_RS18015 0.85 -0.4 3,744,113 + BWI76_RS18015 0.85 -0.4 3,744,132 - BWI76_RS18015 0.88 +1.3 3,744,139 - BWI76_RS18015 0.89 -0.3 3,744,144 + BWI76_RS18015 0.90 +0.7 3,744,157 - +0.2 3,744,157 - +0.1 3,744,184 + -0.1 3,744,192 + +0.2 3,744,192 + -0.3 3,744,258 + -0.1 3,744,259 + -0.7 3,744,261 + +0.2
Or see this region's nucleotide sequence