Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14300 and BWI76_RS14305 are separated by 87 nucleotides BWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotides BWI76_RS14310 and BWI76_RS14315 overlap by 1 nucleotides BWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotides
BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680
_RS14295
BWI76_RS14300: BWI76_RS14300 - hypothetical protein, at 2,989,609 to 2,989,795
_RS14300
BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668
_RS14305
BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554
_RS14310
BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892
_RS14315
BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829
_RS14320
Position (kb)
2990
2991
2992 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2989.701 kb on + strand, within BWI76_RS14300 at 2989.715 kb on - strand, within BWI76_RS14300 at 2989.728 kb on + strand, within BWI76_RS14300 at 2989.731 kb on - strand, within BWI76_RS14300 at 2989.731 kb on - strand at 2989.801 kb on + strand at 2989.806 kb on + strand at 2990.042 kb on + strand, within BWI76_RS14305 at 2990.047 kb on + strand, within BWI76_RS14305 at 2990.321 kb on + strand, within BWI76_RS14305 at 2990.390 kb on + strand, within BWI76_RS14305 at 2990.405 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand at 2990.848 kb on + strand, within BWI76_RS14310 at 2990.850 kb on + strand, within BWI76_RS14310 at 2990.985 kb on + strand, within BWI76_RS14310 at 2991.214 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.294 kb on + strand, within BWI76_RS14310 at 2991.307 kb on + strand at 2991.320 kb on + strand, within BWI76_RS14310 at 2991.326 kb on + strand, within BWI76_RS14310 at 2991.327 kb on + strand, within BWI76_RS14310 at 2991.344 kb on + strand, within BWI76_RS14310 at 2991.350 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.452 kb on + strand, within BWI76_RS14310 at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2992.011 kb on + strand, within BWI76_RS14320 at 2992.114 kb on + strand, within BWI76_RS14320 at 2992.201 kb on + strand, within BWI76_RS14320 at 2992.293 kb on + strand at 2992.293 kb on + strand, within BWI76_RS14320 at 2992.395 kb on + strand, within BWI76_RS14320
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 2,989,701 + BWI76_RS14300 0.49 +0.4 2,989,715 - BWI76_RS14300 0.57 -0.5 2,989,728 + BWI76_RS14300 0.64 -0.5 2,989,731 - BWI76_RS14300 0.65 +1.4 2,989,731 - -0.2 2,989,801 + +0.9 2,989,806 + +1.1 2,990,042 + BWI76_RS14305 0.20 -0.8 2,990,047 + BWI76_RS14305 0.21 +0.2 2,990,321 + BWI76_RS14305 0.56 +0.0 2,990,390 + BWI76_RS14305 0.65 +0.7 2,990,405 + BWI76_RS14305 0.66 +1.8 2,990,471 + BWI76_RS14305 0.75 -2.0 2,990,471 + BWI76_RS14305 0.75 +0.6 2,990,513 + BWI76_RS14305 0.80 +0.6 2,990,513 + BWI76_RS14305 0.80 -1.0 2,990,838 + BWI76_RS14310 0.19 +0.7 2,990,838 + BWI76_RS14310 0.19 +0.2 2,990,840 + BWI76_RS14310 0.19 -1.0 2,990,840 + +1.4 2,990,848 + BWI76_RS14310 0.20 -1.2 2,990,850 + BWI76_RS14310 0.20 -0.9 2,990,985 + BWI76_RS14310 0.35 -0.2 2,991,214 + BWI76_RS14310 0.61 -1.7 2,991,275 + BWI76_RS14310 0.68 -2.2 2,991,275 + BWI76_RS14310 0.68 -0.5 2,991,275 + BWI76_RS14310 0.68 -0.2 2,991,294 + BWI76_RS14310 0.70 +0.9 2,991,307 + +0.8 2,991,320 + BWI76_RS14310 0.73 +0.4 2,991,326 + BWI76_RS14310 0.74 -1.1 2,991,327 + BWI76_RS14310 0.74 +0.3 2,991,344 + BWI76_RS14310 0.76 +2.9 2,991,350 + BWI76_RS14310 0.77 -0.7 2,991,374 + BWI76_RS14310 0.79 -2.0 2,991,374 + BWI76_RS14310 0.79 -1.2 2,991,452 + BWI76_RS14310 0.88 -0.4 2,991,491 + -1.1 2,991,491 + +0.4 2,991,491 + +0.4 2,991,491 + +0.4 2,991,491 + -1.6 2,991,491 + +1.0 2,991,491 + -1.0 2,991,498 + -0.8 2,991,498 + -1.1 2,991,498 + +0.8 2,991,498 + -0.4 2,991,498 + -0.2 2,991,498 + -1.0 2,992,011 + BWI76_RS14320 0.12 -1.0 2,992,114 + BWI76_RS14320 0.24 +1.9 2,992,201 + BWI76_RS14320 0.33 -0.4 2,992,293 + -1.4 2,992,293 + BWI76_RS14320 0.43 -0.2 2,992,395 + BWI76_RS14320 0.54 -1.0
Or see this region's nucleotide sequence