Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14310

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14300 and BWI76_RS14305 are separated by 87 nucleotidesBWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotidesBWI76_RS14310 and BWI76_RS14315 overlap by 1 nucleotidesBWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotides BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680 _RS14295 BWI76_RS14300: BWI76_RS14300 - hypothetical protein, at 2,989,609 to 2,989,795 _RS14300 BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668 _RS14305 BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554 _RS14310 BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892 _RS14315 BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829 _RS14320 Position (kb) 2990 2991 2992Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2989.701 kb on + strand, within BWI76_RS14300at 2989.715 kb on - strand, within BWI76_RS14300at 2989.728 kb on + strand, within BWI76_RS14300at 2989.731 kb on - strand, within BWI76_RS14300at 2989.731 kb on - strandat 2989.801 kb on + strandat 2989.806 kb on + strandat 2990.042 kb on + strand, within BWI76_RS14305at 2990.047 kb on + strand, within BWI76_RS14305at 2990.321 kb on + strand, within BWI76_RS14305at 2990.390 kb on + strand, within BWI76_RS14305at 2990.405 kb on + strand, within BWI76_RS14305at 2990.471 kb on + strand, within BWI76_RS14305at 2990.471 kb on + strand, within BWI76_RS14305at 2990.513 kb on + strand, within BWI76_RS14305at 2990.513 kb on + strand, within BWI76_RS14305at 2990.838 kb on + strand, within BWI76_RS14310at 2990.838 kb on + strand, within BWI76_RS14310at 2990.840 kb on + strand, within BWI76_RS14310at 2990.840 kb on + strandat 2990.848 kb on + strand, within BWI76_RS14310at 2990.850 kb on + strand, within BWI76_RS14310at 2990.985 kb on + strand, within BWI76_RS14310at 2991.214 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.294 kb on + strand, within BWI76_RS14310at 2991.307 kb on + strandat 2991.320 kb on + strand, within BWI76_RS14310at 2991.326 kb on + strand, within BWI76_RS14310at 2991.327 kb on + strand, within BWI76_RS14310at 2991.344 kb on + strand, within BWI76_RS14310at 2991.350 kb on + strand, within BWI76_RS14310at 2991.374 kb on + strand, within BWI76_RS14310at 2991.374 kb on + strand, within BWI76_RS14310at 2991.452 kb on + strand, within BWI76_RS14310at 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2992.011 kb on + strand, within BWI76_RS14320at 2992.114 kb on + strand, within BWI76_RS14320at 2992.201 kb on + strand, within BWI76_RS14320at 2992.293 kb on + strandat 2992.293 kb on + strand, within BWI76_RS14320at 2992.395 kb on + strand, within BWI76_RS14320

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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2,989,701 + BWI76_RS14300 0.49 +0.4
2,989,715 - BWI76_RS14300 0.57 -0.5
2,989,728 + BWI76_RS14300 0.64 -0.5
2,989,731 - BWI76_RS14300 0.65 +1.4
2,989,731 - -0.2
2,989,801 + +0.9
2,989,806 + +1.1
2,990,042 + BWI76_RS14305 0.20 -0.8
2,990,047 + BWI76_RS14305 0.21 +0.2
2,990,321 + BWI76_RS14305 0.56 +0.0
2,990,390 + BWI76_RS14305 0.65 +0.7
2,990,405 + BWI76_RS14305 0.66 +1.8
2,990,471 + BWI76_RS14305 0.75 -2.0
2,990,471 + BWI76_RS14305 0.75 +0.6
2,990,513 + BWI76_RS14305 0.80 +0.6
2,990,513 + BWI76_RS14305 0.80 -1.0
2,990,838 + BWI76_RS14310 0.19 +0.7
2,990,838 + BWI76_RS14310 0.19 +0.2
2,990,840 + BWI76_RS14310 0.19 -1.0
2,990,840 + +1.4
2,990,848 + BWI76_RS14310 0.20 -1.2
2,990,850 + BWI76_RS14310 0.20 -0.9
2,990,985 + BWI76_RS14310 0.35 -0.2
2,991,214 + BWI76_RS14310 0.61 -1.7
2,991,275 + BWI76_RS14310 0.68 -2.2
2,991,275 + BWI76_RS14310 0.68 -0.5
2,991,275 + BWI76_RS14310 0.68 -0.2
2,991,294 + BWI76_RS14310 0.70 +0.9
2,991,307 + +0.8
2,991,320 + BWI76_RS14310 0.73 +0.4
2,991,326 + BWI76_RS14310 0.74 -1.1
2,991,327 + BWI76_RS14310 0.74 +0.3
2,991,344 + BWI76_RS14310 0.76 +2.9
2,991,350 + BWI76_RS14310 0.77 -0.7
2,991,374 + BWI76_RS14310 0.79 -2.0
2,991,374 + BWI76_RS14310 0.79 -1.2
2,991,452 + BWI76_RS14310 0.88 -0.4
2,991,491 + -1.1
2,991,491 + +0.4
2,991,491 + +0.4
2,991,491 + +0.4
2,991,491 + -1.6
2,991,491 + +1.0
2,991,491 + -1.0
2,991,498 + -0.8
2,991,498 + -1.1
2,991,498 + +0.8
2,991,498 + -0.4
2,991,498 + -0.2
2,991,498 + -1.0
2,992,011 + BWI76_RS14320 0.12 -1.0
2,992,114 + BWI76_RS14320 0.24 +1.9
2,992,201 + BWI76_RS14320 0.33 -0.4
2,992,293 + -1.4
2,992,293 + BWI76_RS14320 0.43 -0.2
2,992,395 + BWI76_RS14320 0.54 -1.0

Or see this region's nucleotide sequence