Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13085 and BWI76_RS13090 are separated by 286 nucleotides BWI76_RS13090 and BWI76_RS13095 are separated by 11 nucleotides BWI76_RS13095 and BWI76_RS13100 are separated by 7 nucleotides
BWI76_RS13085: BWI76_RS13085 - bifunctional oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, at 2,729,458 to 2,731,503
_RS13085
BWI76_RS13090: BWI76_RS13090 - phenylacetate-CoA oxygenase subunit PaaA, at 2,731,790 to 2,732,719
_RS13090
BWI76_RS13095: BWI76_RS13095 - phenylacetate-CoA oxygenase subunit PaaB, at 2,732,731 to 2,733,018
_RS13095
BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781
_RS13100
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-1
0
1 at 2731.077 kb on - strand, within BWI76_RS13085 at 2731.806 kb on + strand at 2731.992 kb on - strand, within BWI76_RS13090 at 2732.023 kb on - strand, within BWI76_RS13090 at 2732.123 kb on + strand, within BWI76_RS13090 at 2732.224 kb on - strand, within BWI76_RS13090 at 2732.879 kb on + strand, within BWI76_RS13095 at 2732.982 kb on + strand, within BWI76_RS13095 at 2732.990 kb on - strand at 2733.007 kb on + strand at 2733.042 kb on - strand at 2733.362 kb on + strand, within BWI76_RS13100 at 2733.402 kb on + strand, within BWI76_RS13100 at 2733.453 kb on + strand, within BWI76_RS13100 at 2733.541 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 2,731,077 - BWI76_RS13085 0.79 -1.2 2,731,806 + +0.3 2,731,992 - BWI76_RS13090 0.22 -0.4 2,732,023 - BWI76_RS13090 0.25 -0.4 2,732,123 + BWI76_RS13090 0.36 -0.4 2,732,224 - BWI76_RS13090 0.47 -1.3 2,732,879 + BWI76_RS13095 0.51 -0.4 2,732,982 + BWI76_RS13095 0.87 -0.7 2,732,990 - +0.3 2,733,007 + +0.1 2,733,042 - +0.7 2,733,362 + BWI76_RS13100 0.44 +0.2 2,733,402 + BWI76_RS13100 0.50 -0.5 2,733,453 + BWI76_RS13100 0.56 -0.1 2,733,541 + BWI76_RS13100 0.68 -0.4 2,733,580 + BWI76_RS13100 0.73 -1.5 2,733,580 + BWI76_RS13100 0.73 +0.1
Or see this region's nucleotide sequence