Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10475

Experiment: K. M5a1 KLEB23 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10470 and BWI76_RS10475 are separated by 23 nucleotidesBWI76_RS10475 and BWI76_RS10480 are separated by 0 nucleotides BWI76_RS10470: BWI76_RS10470 - iron ABC transporter substrate-binding protein, at 2,211,755 to 2,212,771 _RS10470 BWI76_RS10475: BWI76_RS10475 - binding-protein-dependent transport system inner membrane protein, at 2,212,795 to 2,214,369 _RS10475 BWI76_RS10480: BWI76_RS10480 - amino acid ABC transporter substrate-binding protein, at 2,214,370 to 2,215,398 _RS10480 Position (kb) 2212 2213 2214 2215Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2211.929 kb on - strand, within BWI76_RS10470at 2211.937 kb on + strand, within BWI76_RS10470at 2211.945 kb on - strand, within BWI76_RS10470at 2212.080 kb on + strand, within BWI76_RS10470at 2212.687 kb on + strandat 2212.801 kb on + strandat 2212.889 kb on + strandat 2213.006 kb on + strand, within BWI76_RS10475at 2213.299 kb on + strand, within BWI76_RS10475at 2213.357 kb on - strand, within BWI76_RS10475at 2213.968 kb on - strand, within BWI76_RS10475at 2214.160 kb on + strand, within BWI76_RS10475at 2214.242 kb on + strandat 2214.616 kb on + strand, within BWI76_RS10480at 2214.725 kb on + strand, within BWI76_RS10480at 2214.753 kb on + strand, within BWI76_RS10480at 2214.787 kb on - strand, within BWI76_RS10480at 2214.854 kb on - strand, within BWI76_RS10480at 2214.915 kb on + strand, within BWI76_RS10480at 2214.923 kb on - strand, within BWI76_RS10480at 2214.941 kb on + strand, within BWI76_RS10480at 2214.978 kb on + strand, within BWI76_RS10480at 2215.011 kb on + strand, within BWI76_RS10480at 2215.029 kb on + strand, within BWI76_RS10480at 2215.037 kb on - strand, within BWI76_RS10480at 2215.037 kb on - strandat 2215.197 kb on + strand, within BWI76_RS10480at 2215.358 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1
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2,211,929 - BWI76_RS10470 0.17 -2.9
2,211,937 + BWI76_RS10470 0.18 +0.5
2,211,945 - BWI76_RS10470 0.19 +0.1
2,212,080 + BWI76_RS10470 0.32 +1.0
2,212,687 + -0.5
2,212,801 + -0.2
2,212,889 + -0.7
2,213,006 + BWI76_RS10475 0.13 -0.5
2,213,299 + BWI76_RS10475 0.32 +0.4
2,213,357 - BWI76_RS10475 0.36 -1.0
2,213,968 - BWI76_RS10475 0.74 -1.5
2,214,160 + BWI76_RS10475 0.87 -0.2
2,214,242 + -0.5
2,214,616 + BWI76_RS10480 0.24 -1.4
2,214,725 + BWI76_RS10480 0.34 +2.3
2,214,753 + BWI76_RS10480 0.37 -0.0
2,214,787 - BWI76_RS10480 0.41 +0.0
2,214,854 - BWI76_RS10480 0.47 -0.4
2,214,915 + BWI76_RS10480 0.53 +0.6
2,214,923 - BWI76_RS10480 0.54 +0.1
2,214,941 + BWI76_RS10480 0.55 -0.1
2,214,978 + BWI76_RS10480 0.59 +0.3
2,215,011 + BWI76_RS10480 0.62 -0.1
2,215,029 + BWI76_RS10480 0.64 +0.1
2,215,037 - BWI76_RS10480 0.65 -0.8
2,215,037 - -1.1
2,215,197 + BWI76_RS10480 0.80 +0.0
2,215,358 + +0.3

Or see this region's nucleotide sequence